CLTC/CLTCL1 Recombinant Monoclonal Antibody

Clathrin heavy chain proteins CLTC and CLTCL1 serve as essential components of the clathrin-mediated endocytosis machinery, forming the characteristic triskelion structures that drive vesicle formation at the plasma membrane and trans-Golgi network. These proteins are fundamental to receptor internalization, nutrient uptake, and synaptic vesicle recycling, making them critical targets for researchers investigating membrane trafficking, signal transduction, and neurobiology.

This recombinant monoclonal antibody, clone 15H3, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in a controlled recombinant system, you can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations.

Validation data demonstrates robust performance across multiple applications and sample types. Western blot analysis confirms detection of the expected 192 kDa band across a diverse panel of human cell lines including HeLa, K562, HepG2, HL-60, Jurkat, and HEK293, as well as mouse NIH/3T3 cells and rat brain tissue, providing confidence in cross-species reactivity for human, mouse, and rat samples. Immunohistochemistry staining has been validated in paraffin-embedded mouse kidney tissue and human breast cancer sections using standard citrate buffer antigen retrieval, while flow cytometry analysis in MCF-7 cells demonstrates clear positive signal separation from isotype controls.

The unconjugated format and broad working dilution ranges across applications give you flexibility to optimize detection for your specific experimental conditions. Whether you are studying endocytic pathway dynamics, investigating clathrin's role in disease pathology, or characterizing trafficking defects, this antibody provides a reliable tool for your research.