CHUK Recombinant Monoclonal Antibody

Code: CSB-RA916058A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, WB, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O15111

Target Names

CHUK

Alternative Names

chuk antibody; CHUK1 antibody; Conserved Helix Loop Helix Ubiquitous Kinase antibody; Conserved helix loop ubiquitous kinase antibody; Conserved helix-loop-helix ubiquitous kinase antibody; I Kappa B Kinase 1 antibody; I Kappa B Kinase Alpha antibody; I-kappa-B kinase 1 antibody; I-kappa-B kinase alpha antibody; IkappaB kinase antibody; IkB kinase alpha subunit antibody; IkBKA antibody; IKK 1 antibody; IKK A antibody; IKK a kinase antibody; IKK-A antibody; IKK-alpha antibody; IKK1 antibody; IKKA antibody; IKKA_HUMAN antibody; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody; Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody; Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody; NFKBIKA antibody; Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody; Nuclear factor NF kappa B inhibitor kinase alpha antibody; Nuclear factor NF-kappa-B inhibitor kinase alpha antibody; Nuclear factor NFkappaB inhibitor kinase alpha antibody; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody; TCF-16 antibody; TCF16 antibody; Transcription factor 16 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human CHUK

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4H4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HEK293 whole cell lysate(30µg), A549 whole cell lysate(30µg), A-431 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HeLa whole cell lysate(30µg), PC-3 whole cell lysate(30µg), Mouse brain tissue lysate(30µg)
All lanes: IKK alpha antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 2min
Overlay Peak curve showing HepG2 cells stained with https://www.cusabio.cn/images/antibody/Recombinant/CSB-RA916058A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

CHUK, also known as IKK-alpha or IKK1, serves as a critical catalytic subunit of the IκB kinase complex that orchestrates NF-κB activation. This serine/threonine kinase phosphorylates inhibitors of NF-κB, triggering their degradation and enabling NF-κB transcription factors to translocate to the nucleus and drive expression of genes involved in inflammation, immunity, cell survival, and proliferation. Beyond its canonical role in NF-κB signaling, CHUK participates in diverse cellular processes including keratinocyte differentiation, mammary gland development, and skeletal morphogenesis, making it a compelling target for researchers investigating inflammatory diseases, cancer biology, and developmental pathways.

This recombinant monoclonal antibody, generated in rabbit using clone 4H4, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than hybridoma-derived sources, you can expect reliable performance across experiments and between lots, eliminating a common source of variability in longitudinal studies.

Validation data demonstrates robust performance across multiple applications. In western blot analysis, the antibody detects a band at the predicted molecular weight of 85 kDa across a diverse panel of human cell lines including HEK293, A549, A-431, COLO205, HeLa, and PC-3, as well as mouse brain tissue lysate, confirming cross-species reactivity between human and mouse samples. Flow cytometry validation in HepG2 cells shows clear separation from isotype control, supporting intracellular detection applications.

The unconjugated format and affinity-purified preparation provide flexibility for pairing with your preferred detection systems. Whether you are mapping NF-κB pathway dynamics, investigating inflammatory signaling in disease models, or characterizing kinase expression across tissue types, this antibody delivers the specificity and versatility your research demands.

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Target Background

Function(From Uniprot)

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates AMBRA1 following mitophagy induction, promoting AMBRA1 interaction with ATG8 family proteins and its mitophagic activity.

Gene References into Functions

evidence that DCNL5 may be involved in innate immunity, as it is a direct substrate of the kinase IKKalpha during immune signalling. PMID:29958295
cFLIP appears to bind to IKKalpha to prevent IKKalpha from phosphorylating and activating IRF7. PMID:29222334
functions as a suppressor of lung adenocarcinoma; deletion up-regulates NOX2 and down-regulates NRF2, leading to ROS accumulation and blockade of cell senescence induction PMID:29311298
HOTAIR operates the action of IKKalpha, IKKbeta, IKKgamma in liver cancer stem cells PMID:27367027
Results indicate the involvement of IKK and NF-kappaB signaling in the maintenance of glioblastoma stem cell. PMID:27732951
Loss-of-function of LINC00473 in vivo effectively promoted the regression of Wilms tumour via miR-195/IKKalpha-mediated growth inhibition. PMID:29159834
Study results provide new insights into the molecular mechanisms of maspin suppression in response to HBx, and revealed nuclear IKKalpha as a prognostic biomarker and a potential therapeutic target to improve the clinical outcome of HBV-associated HCC patients. PMID:27409165
Data show that IKKalpha directly bind to the promoters of LGR5, in turn, upregulating LGR5 expression through activation of STAT3 signaling pathway during cancer progression. PMID:27049829
single-particle cryoelectron microscopy (cryo-EM) and X-ray crystal structures of human IKK1 in dimeric ( approximately 150 kDa) and hexameric ( approximately 450 kDa) forms, are reported. PMID:27851956
results suggest that changes in the relative concentrations of RelB, NIK:IKK1, and p100 during noncanonical signaling modulate this transitional complex and are critical for maintaining the fine balance between the processing and protection of p100. PMID:27678221
IKKalpha-dependent phosphorylation of S376 stimulated whereas IKKalpha-independent phosphorylation of S484 inhibited RORgammat function in Th17 differentiation. PMID:28667162
IKKalpha promotes migration through dynamic interactions with the EGF promoter depending on the redox state within cells. PMID:28122935
the dual regulation of STAT1 by IKKalpha in antiviral signaling suggests a role for IKKalpha in the fine-tuning of antiviral signaling in response to non-self RNA. PMID:27992555
In epithelial ovarian cancer cells, miR-23a enhances the expression of IKKalpha. The proliferation, migration, and invasion of EOC cells are increased by IKKalpha. PMID:27537390
TLR signaling led to lower expression of LRRC14. PMID:27426725
IKKalpha is an important determinant of poor outcome in patients with ER-positive invasive ductal breast cancer and thus may represent a potential therapeutic target PMID:28006839
The molecular mechanisms involved in IKKalpha-related tumors. [review] PMID:26323241
this study shows that miR-23a regulated IL-17-mediated proinflammatory mediators expression in rheumatoid arthritis by directly targeting IKKalpha PMID:27936459
W346 effectively inhibited tumor necrosis factor (TNF-a)-induced NF-kappaB activation by suppressing IKK phosphorylation, inhibiting IkB-a degradation, and restraining the accumulation of NF-kappaB subunit p65 nuclear translocation. W346 also affected NF-kappaB-regulated downstream products involved in cycle arrest and apoptosis. PMID:26520440
Data indicate a significantly higher quantity of mesenchymal stromal cells (MSCs)was produced from human embryonic stem cells (hESCs) with IkappaB kinase (IKK)/nuclear factor kappa B (NF-kappaB) suppression. PMID:26972683
Findings indicate that IkappaB kinase inhibitor ACHP can slowdown the accumulation of collagen type I (COL1A1). PMID:26337045
TRIM22 could interact with IkappaB kinase (IKK)alpha but not IKKbeta and could increase the level and phosphorylation of IKKalpha through its really interesting new gene (RING) and spla-ryanodine receptor (SPRY) domains. PMID:25510414
IKKalpha is diversely expressed in keratinizing and non-keratinizing carcinomas in the same type of cancer. PMID:26317791
results indicate that nuclear active IKK is a robust biomarker to predict cutaneous squamous cell carcinoma outcome, and suggest the possibility of targeting IKK activity as a future therapy for treating metastatic cutaneous squamous cell carcinomas PMID:26094020
We also report that the extra-genomic effects elicited by both ligands, leading to recruitment of active Akt to chromatin, are essential for phosphorylation of serine 10 in histone H3 by IKKa PMID:25482200
Upon infection, the HCV 3'UTR redistributes DDX3X and IKK-alpha to speckle-like cytoplasmic structures shown to be stress granules. PMID:25740981
Suppression of PKK expression by RNA interference inhibits phosphorylation of IKKalpha and IKKbeta as well as activation of NF-kappaB in human cancer cell lines; thus, PKK regulates NF-kappaB activation by modulating activation of IKKalpha and IKKbeta. PMID:25096806
Silencing of IKKa in E2-challenged cells resulted into an increased presence of either 8-oxo-Gs as well as of the base excision repair enzyme 8-oxo-guanine-DNA glycosylase 1. PMID:24971480
Findings indicated that IKKalpha plays a crucial role as a tumor suppressor that suppresses the invasion, metastasis, and angiogenesis of nasopharyngeal carcinoma (NPC) cells in vitro and correlates with the survival in NPC patients. PMID:24753359
Authors show that activation of NF-kappaB by Kaposi's sarcoma-associated herpesvirus K15 protein involves the recruitment of NF-kappaB-inducing kinase (NIK) and IKK alpha/beta to result in the phosphorylation of p65/RelA on Ser536. PMID:25187543
Data show that downstream of Akt protein, IkappaB kinase alpha (IKKalpha) directly phosphorylates mammalian target of rapamycin (mTOR) to drive mTORC1 activation. PMID:24990947
Survivin-2B promoted autophagy and further regulated cell death by accumulating and stabilizing IKK alpha in the nucleus of selenite treated leukemia cells. PMID:24556686
IKK complex functions as a key mediator of detachment-induced autophagy and anoikis resistance in epithelial cells. PMID:23778976
Increases in Ikappa B kinase alpha suppresses the progression of nasopharyngeal carcinoma. PMID:24075781
That block IKKalpha/beta and EGFR pathways. PMID:23455325
Data indicate that muscle IKK-alpha protein content was significantly lower in chronic obstructive pulmonary disease (COPD) patients. PMID:24215713
IKK interacts with rictor and regulates the function of mTORC2 including phosphorylation of AKT (at Serine473) and organization of actin cytoskeleton. PMID:23872070
roles for p53 and IKKalpha/IKKbeta in non-canonical Notch signaling and IL-6 as a novel non-canonical Notch target gene PMID:23178494
Data indicate that eight of twelve compounds showed acceptable inhibitory effects on IKKbeta. PMID:23501112
Results suggest that 13-197 targets IKKbeta and thereby inhibits mTOR and NF-kappaB pathways. PMID:23444213
Our findings suggest ERLIN1-CHUK-CWF19L1 variants are associated with early stage of fatty liver accumulation to hepatic inflammation. PMID:23477746
Results demonstrate that IL-8 expression is mediated, at least partly, by IKKalpha PMID:23894194
Reconstituting irradiated mutant animals with wild-type bone marrow (BM) prevented SCC development, suggesting that BM-derived IKKalpha mutant macrophages promote the transition of IKKalpha(low)K5(+)p63(hi) cells to tumor cells. PMID:23597566
This review highlights major advances in the studies of the nuclear functions of IKKalpha and the mechanisms of IKKalpha nuclear translocation. Understanding the nuclear activity is essential for targeting IKKalpha for therapeutics. PMID:23343355
Chemical inhibitors of IKK-alpha suppress HCV infection and IKK-alpha-induced lipogenesis, offering a proof-of-concept approach for new HCV therapeutic development PMID:23708292
Inhibition of IKKalpha partially rescued p53 levels, while concomitant IKKalpha inhibition fully rescued p53 and regulates MDM2 SUMOylation. PMID:23032264
H5N1 virus NS1 not only blocks IKKbeta-mediated phosphorylation and degradation of IkappaBalpha in the classical pathway but also suppresses IKKalpha-mediated processing of p100 to p52 in the alternative pathway. PMID:22891964
The results suggest that inactivation of IKKalpha, followed by Akt and FOXO1 phosphorylation and caspase-3 activation, contributes to zerumbone-induced GBM cell apoptosis. PMID:23035900
Active nuclear p45-IKKalpha forms a complex with nonactive IKKalpha and NEMO that mediates phosphorylation of SMRT and histone H3. PMID:23041317
E7 proteins from the cutaneous human papillomavirus types demonstrated interaction with IKKalpha but not with IKKbeta. PMID:22776252