CHAT Recombinant Monoclonal Antibody

Code: CSB-RA825574A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, WB, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P28329

Target Names

CHAT

Alternative Names

Choline O-acetyltransferase (CHOACTase) (ChAT) (Choline acetylase) (EC 2.3.1.6), CHAT

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from Human CHAT

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8E7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Mouse Brain tissue lysate
All lanes: CHAT antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
IHC image of CSB-RA825574A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.22% DAB.
Immunofluorescence staining of SH-SY5Y with CSB-RA825574A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 498-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA825574A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Choline O-acetyltransferase (CHAT) serves as the rate-limiting enzyme in acetylcholine biosynthesis, catalyzing the transfer of an acetyl group from acetyl-CoA to choline. As a definitive marker for cholinergic neurons, CHAT plays a central role in neuroscience research spanning neurodegenerative disease, synaptic function, and neural circuit mapping. Reliable detection of this enzyme is essential for researchers investigating cholinergic signaling pathways and their dysfunction in conditions such as Alzheimer's disease.

This recombinant monoclonal antibody, clone 8E7, offers the consistency and reproducibility that demanding neuroscience applications require. Produced using recombinant technology with a sequence-defined rabbit IgG format, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The defined sequence ensures that your experimental conditions remain transferable across projects and publications.

Validation studies demonstrate robust performance across multiple detection platforms. Western blot analysis of mouse brain tissue lysate reveals a clean band at the predicted 70 kDa molecular weight, confirming specific target recognition at dilutions of 1:500. The antibody performs effectively in immunohistochemistry, as shown in paraffin-embedded human lung cancer tissue sections, and delivers clear immunofluorescence staining in SH-SY5Y neuroblastoma cells, a widely used model for cholinergic neuron research. Flow cytometry validation in SH-SY5Y cells further demonstrates utility for quantitative single-cell analysis, with clear separation from isotype control.

With cross-species reactivity for human and mouse samples and compatibility spanning ELISA, Western blot, immunohistochemistry, immunofluorescence, and flow cytometry, this antibody provides the experimental flexibility needed for comprehensive cholinergic system investigations.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Gene References into Functions

the AA genotype of CHAT was associated with a 1.25 times higher risk of Alzheimer's disease (AD) thus demonstrating that the rs3810950 polymorphism can have a modest but statistically significant effect on the risk of AD in the Czech population PMID:29759072
Compared with the control group, the densitometric quantification and mean density of GPR43 and ChAT proteins, and expression of GPR43 and CHAT genes, were significantly decreased in the patients with mixed refractory constipation. PMID:26921846
meta-analysis suggested that rs1880670G/A, and rs2177369 G/A polymorphisms were not risk factors for Alzheimer's Disease. However, rs3810950G/A, or rs868750G/A genetic polymorphism was a genetic risk factor for the development of Alzheimer's Disease. PMID:27390868
Results suggest that SATB1 is activated to bind to chromatin at S/MARs after exposure to Abeta 1-42, resulting in alternative utilization and movement of 82-kDa ChAT to these regions demonstrating that both proteins play critical roles in the response of neural cells to acute Abeta-exposure. PMID:27052102
We conducted a meta-analysis of studies involving CHAT, TFAM, and VR22 polymorphisms and Alzheimer disease susceptibility.For CHAT, rs2177369 (G>A) in whites and rs3810950 (G>A) in Asians were found to be associated with AD susceptibility. No association was detected between rs1880676 and rs868750 and AD risk. PMID:27272392
In conclusion, our meta-analysis indicated CHAT rs2177369 polymorphism might play a protective role in AD, while rs3810950 variant was a risk factor for AD but its single heterozygous mutations might not influence susceptibility to AD. PMID:27597977
Two novel CHAT gene mutations, p.Val306Leu and p.Ser704del were detected in an ethnic Kadazandusan family with congenital myasthenic syndrome. PMID:26789281
sequence variants of CHAT were associated with human cognitive ability in not only patients with psychiatric disorders but also normal healthy individuals PMID:26854842
There is a striking variability in the severity of phenotypes resulting from mutations in CHAT, which is the only gene so far known to be linked with congenital deficiency of ACh synthesis. PMID:26080897
Data show thata the expression of choline acetyltransferase (ChAT) is reduced in the postmortem alcoholic basal forebrain in comparison to moderate drinking controls. PMID:25405505
This study confirmed that genetic polymorphism of CHAT has an influence on drug response in Alzheimer's disease. PMID:25730470
These studies indicate a novel relationship between cholinergic neurons and APP processing, with 82-kDa ChAT acting as a negative regulator of Abeta production PMID:24844149
The results demonstrate that human NSCs over-expressing ChAT improve cognitive function and physical activity of aging mice. PMID:23731954
rs1880676 is functional and the allelic variations of this polymorphism are involved in developing nicotine dependence by altering ChAT expression. PMID:24076142
There was a loss of choline O-acetyltransferase in the visual cortex of dementia with Lewy bodies patients. PMID:23242284
There were CHAT mRNA reactions in the synovial lining layer in patients with rheumatoid arthritis and osteoarthritis. PMID:22483691
In airway epithelial cells anti-CHAT immunogold was found particularly within the apical cell membrane, cilia, submucosa, cytosol and nuclear membrane. PMID:22683430
Data from transgenic mice expressing human CHAT in brain neurons suggest that CHAT is important in maintaining memory and learning throughout life. PMID:22449376
[review] The peripheral type of ChAT appears to be a reliable marker for the visualization of peripheral cholinergic neurons and their processes, whereas other conventional markers including the common ChAT have not been used successfully for it. PMID:21382474
The CHAT A/A genotype was associated with earlier onset of Alzheimer disease. PMID:21602657
the functional consequences of 12 missense and one nonsense mutations of CHAT in 11 patients. ( choline acetyltransferase) PMID:21786365
Multiple abnormalities with intellectual and developmental disability result from recurrent deletions and reciprocal duplications of 10q11.21q11.23 including CHAT and SLC18A3. PMID:21948486
The ChAT rs3810950 A allele was found to be associated with a decrease cognitive status evaluated by a five-component cognitive composite score PMID:21883924
Multiple sclerosis hippocampus, activity and protein expression of choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme, was decreased, while the activity and protein expression of acetylcholinesterase PMID:21691765
The data of this study did not seem to support a major role for CHAT genetic variation in geriatric depression and Alzheimer's disease , there might be a minor contribution in geriatric patients with depression. PMID:21507424
overexpressed ChAT enhanced transcription of the CHT1 gene but not the VACHT gene PMID:21163949
residues M84, Y436, and Y552 play a critical role in binding and holding the choline substrate in the ChAT active site. PMID:20560540
Although the effect sizes in both cohorts are modest, converging data across cohorts and phenotypes suggest that ChAT may be involved in nicotine dependence and ability to quit smoking. PMID:20147892
Replication and association of CHAT with nicotine dependence in European and African American smokers are reported. PMID:20383528
the acquisition of neurotransmitter phenotype is epigenetically, at least the hyper-acetylation on the core promoter region of ChAT gene, regulated in NG108-15 neuronal cells PMID:20100532
Results suggest that c-Myb and C/EBPbeta act synergistically to increase choline acetyltransferase gene transcription in the nervous system. PMID:12393272
A G-to-A polymorphism detected in the first coding exon of the ChAT sequence may result in attenuated translation efficiency of ChAT mRNA and confer an increased risk for deterioration of memory and cognition functions in Alzheimer's disease. PMID:12401548
we identified a novel missense mutation (I336T) in the CHAT gene homozygously in all three patients. Haplotype analysis revealed that the mutant allele cosegregates with the clinical phenotype in both families. PMID:12609506
the wide existence of ChAT in human endothelial cells PMID:12628465
identification of a novel nuclear localization signal common to 69- and 82-kDa isoforms PMID:12637523
Levels of linkage disequilibrium were generally low across the CHAT locus but two of the coding variants, D7N and A120T, proved to be in complete linkage disequilibrium (late-onset Alzheimer disease). PMID:12759818
previously identified polymorphism in choline acetyltransferase is not associated with Alzheimer's disease PMID:12770689
Choline acetyltransferase neurons in the human parietal neocortex, strongly supports the existence of intrinsic cholinergic innervation of the human neocortex PMID:14514417
ChAT is differentially phosphorylated by protein kinase C isoforms on four serines (Ser-440, Ser-346, Ser-347, and Ser-476) and one threonine (Thr-255) PMID:15381704
These findings show that significant thalamic presynaptic cholinergic deficits occur only in cases of combined cortical and subcortical neurodegeneration in which dementia developed after prolonged parkinsonism. PMID:15913843
one SNP, rs733722, in a promoter region of CHAT, is associated with response of AD patients to cholinesterase inhibitors PMID:16424819
No relationship between pattern of cholinergic deficits and distribution pattern of lesions in amygdala of patients with Alzheimer's disease or dementia with Lewy bodies. PMID:16468020
There is considerable effect of the ChAT polymorphisms on Alzheimer's disease in Korean population and this effect is dependent on apolipoprotein E genotypes PMID:16480703
Structure of human ChAT to a resolution of 2.2 A along with structures for binary complexes of ChAT with choline, coenzyme A and a nonhydrolyzable acetyl-CoA analogue. PMID:17144655
This multiplex PCR technique can be carried out in a single tube and can differentiate between the three polymorphic sites of this gene associated with Alzheimer's disease. PMID:17378730
ChAT, polymorphisms do not constitute a major genetic risk factor for susceptibility to Alzheimer's disease in a Sardinian population. PMID:17503475
findings tentatively implicate a genetic influence of ChAt in the disorder's susceptibility. PMID:17503482
From a panel of 59 single-nucleotide polymorphisms (SNPs) located on 11 candidate genes, we identify four SNPs (one each on CHRNA5 and CHRNA2 and two on CHAT) that appear to have pharmacogenetic relevance in smokin cessation therapy. PMID:18165968
Alzheimer's disease nucleus basalis forebrain neurons, hyperinnervated by galanin, displays a significant upregulation in choline acetyltransferase. PMID:18322398
These results indicate that basal forebrain cholinergic neuron abnormalities are present very early in aging and in the course of Alzheimer disease. PMID:18379437