CD53 Recombinant Monoclonal Antibody

Code: CSB-RA439535A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

P19397

Target Names

CD53

Alternative Names

Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53) (Tetraspanin-25) (Tspan-25) (CD antigen CD53), CD53, MOX44 TSPAN25

Species Reactivity

Human

Immunogen

A synthesized peptide from human CD53 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7F6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol Immunohistochemistry (IHC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA439535A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

CD53 is a member of the tetraspanin superfamily, a group of transmembrane proteins that organize membrane microdomains and regulate diverse cellular processes including adhesion, migration, and signal transduction. Expressed predominantly on leukocytes, CD53 plays important roles in immune cell function and has been implicated in lymphocyte activation, T cell co-stimulation, and inflammatory responses. Its involvement in immune regulation makes it a valuable target for researchers investigating hematological malignancies, autoimmune conditions, and the tumor microenvironment.

This recombinant monoclonal antibody, clone 7F6, offers the consistency and reliability that demanding research applications require. Produced using recombinant technology with a sequence-defined rabbit IgG framework, it eliminates the lot-to-lot variability that can compromise longitudinal studies and reproducibility. The antibody was raised against a synthetic peptide derived from human CD53, ensuring precise epitope targeting.

Validation in immunohistochemistry demonstrates clear performance in paraffin-embedded human pancreatic cancer tissue, where the antibody was tested at 1:100 dilution using a standard citrate buffer antigen retrieval protocol. This validation in tumor tissue is particularly relevant for researchers examining CD53 expression patterns in cancer contexts or investigating immune cell infiltration within the tumor microenvironment. The recommended working dilution range of 1:50-1:200 for IHC provides flexibility for optimization across different tissue types and detection systems.

Whether you are characterizing leukocyte populations in tissue sections, exploring tetraspanin biology in cancer research, or investigating immune cell dynamics, this antibody provides a dependable tool for advancing your understanding of CD53 function in human samples.

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Target Background

Function(From Uniprot)

Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells.

Gene References into Functions

In this study, significant and lineage-dependent association of CD53 locus with Tuberculosis (TB) onset was identified from the pathogen lineage-based genome-wide association study (GWAS). PMID:28878339
CD53 is associated with population asthma risk via the functional promoter polymorphism -1560 C>T. PMID:23313165
SNP rs6679497 in gene CD53 showed significant association with TNFalpha levels (P=0.001); no association of this SNP was observed with osteoarthritis PMID:20407468
Ligation of CD53 triggers a survival response and reduces the number of cells that enter apoptosis. The CD53 antigen interactions might contribute to cell survival in poorly vascularized regions of the tumor mass. PMID:12606948
Analysis of lymphocyte subsets showed that the total number of CD3+, CD4+, CD8+ and CD56+ lymphocytes increased after the intensive protocol before falling below pre-exercise values at 1 h post-exercise. PMID:16506060
The paradox is that lymphocytes from RA patients are believed to resist apoptosis, and we suggest that the elevated expression of CD53, which results from the increased oxidative stress, may protect against apoptosis. PMID:17210617