CD3D Recombinant Monoclonal Antibody

Code: CSB-RA004930MA1HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

P04234

Target Names

CD3D

Alternative Names

4930549J05Rik antibody; A430104F18Rik antibody; AW552088 antibody; Cd247 antibody; CD247 antigen antibody; CD247 antigen; zeta subunit antibody; CD247 molecule antibody; CD3 antibody; CD3 antigen; delta subunit antibody; CD3 delta antibody; CD3 epsilon antibody; CD3 eta antibody; CD3 gamma antibody; CD3 molecule delta polypeptide antibody; CD3 molecule; epsilon polypeptide antibody; CD3 molecule; gamma polypeptide antibody; CD3 zeta antibody; CD3-DELTA antibody; CD3d antibody; CD3D antigen delta polypeptide antibody; CD3d antigen; delta polypeptide (TiT3 complex) antibody; CD3d molecule delta antibody; CD3d molecule delta CD3 TCR complex antibody; CD3d molecule; delta (CD3-TCR complex) antibody; CD3D_HUMAN antibody; CD3E antibody; CD3e antigen antibody; CD3E antigen epsilon polypeptide antibody; CD3e antigen; epsilon polypeptide (TiT3 complex) antibody; CD3e molecule epsilon antibody; CD3e molecule epsilon CD3 TCR complex antibody; CD3e molecule; epsilon (CD3-TCR complex) antibody; CD3epsilon antibody; CD3G antibody; CD3g antigen antibody; CD3G antigen gamma polypeptide antibody; CD3g antigen; gamma polypeptide (TiT3 complex) antibody; CD3g molecule gamma antibody; CD3g molecule gamma CD3 TCR complex antibody; CD3g molecule; gamma (CD3-TCR complex) antibody; CD3H antibody; CD3Q antibody; CD3Z antibody; CD3zeta antibody; Ctg3 antibody; FLJ17620 antibody; FLJ17664 antibody; FLJ18683 antibody; FLJ79544 antibody; FLJ94613 antibody; IMD19 antibody; Leu-4 antibody; MGC138597 antibody; OKT3; delta chain antibody; OTTHUMP00000032544 antibody; T cell receptor antibody; T cell receptor T3 delta chain antibody; T cell receptor T3 gamma chain antibody; T cell receptor T3 zeta chain antibody; T cell receptor zeta chain antibody; T cell surface antigen T3/Leu 4 epsilon chain antibody; T cell surface glycoprotein CD3 antibody; T cell surface glycoprotein CD3 delta chain antibody; T cell surface glycoprotein CD3 epsilon chain antibody; T cell surface glycoprotein CD3 gamma chain antibody; T cell surface glycoprotein CD3 zeta chain antibody; T-cell antigen receptor complex; delta subunit of T3 antibody; T-cell antigen receptor complex; epsilon subunit of T3 antibody; T-cell antigen receptor complex; gamma subunit of T3 antibody; T-cell antigen receptor complex; zeta subunit of CD3 antibody; T-cell receptor T3 delta chain antibody; T-cell receptor T3 gamma chain antibody; T-cell surface antigen T3/Leu-4 epsilon chain antibody; T-cell surface glycoprotein CD3 delta chain antibody; T-cell surface glycoprotein CD3 epsilon chain antibody; T-cell surface glycoprotein CD3 gamma chain antibody; T3 antibody; T3d antibody; T3e antibody; T3g antibody; T3z antibody; TCRE antibody; TCRk antibody; Tcrz antibody; TCRzeta antibody

Species Reactivity

Human

Immunogen

Recombinant Human CD3D protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

8H8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: CD3D antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa

Description

CD3D (CD3 delta) serves as an essential component of the T cell receptor complex, playing a critical role in T cell development, activation, and signal transduction. As part of the CD3 signaling apparatus, this protein is fundamental to adaptive immune responses, making it a key target for researchers investigating T cell biology, immunotherapy development, and immune-related disorders.

This recombinant monoclonal antibody, clone 8H8, offers the reproducibility and consistency that demanding immunological research requires. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, you can expect reliable lot-to-lot performance across long-term studies and multi-site collaborations. The human IgG1 isotype format and affinity chromatography purification ensure high specificity for your target while minimizing background interference.

Validated for Western blot and ELISA applications, this antibody provides flexibility across common analytical workflows. Western blot testing in Jurkat whole cell lysate, a well-established human T cell line, demonstrates clean detection of CD3D at the predicted 19 kDa molecular weight, confirming accurate target recognition. For Western blot applications, dilutions ranging from 1:500 to 1:2000 offer optimization flexibility depending on your experimental system and detection method.

The antibody is supplied in a liquid format containing 50% glycerol in PBS with 0.03% Proclin 300 preservative, ready for immediate use without reconstitution. Long-term storage at -20°C or -80°C maintains stability, though repeated freeze-thaw cycles should be avoided to preserve performance.

This CD3D antibody supports research spanning immunology and stem cell biology, particularly studies examining T cell differentiation, TCR complex assembly, and immune cell characterization.

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Target Background

Function(From Uniprot)

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).

Gene References into Functions

PCR and transcriptome analysis suggest that the genes CD3D and PKRCQ together can be used as a model for differentiating between B-cell and T-cell acute lymphoblastic leukemia. PMID:27494091
we demonstrate that the incorporation of a 2A self-processing peptide derived from foot-and-mouth disease virus conveying co-translational cleavage into a two-chain anti-CD3 x anti-CEA diabody gene enables near-equimolar expression of diabody chains 1 and 2, and thus increases the final amount of assembled diabody. PMID:28075428
A FOXP3(+)CD3(+)CD56(+)-expressed T-cell population with immunosuppressive function and reduced patient survival has been identified in cancer tissues of human hepatocellular carcinoma. PMID:26437631
The docking site for CD3 subunits on the T Cell receptor beta chain has been identified by solution NMR. PMID:26109064
analysis of the molecular organization of the TCR-CD3 complex PMID:25422432
Two cases of SCID with CD3delta gene mutation in Mexican Mennonite infants are described. PMID:24288697
The surface TCR expression of primary alphabeta and gammadelta T cells from healthy donors carrying a single null or leaky mutation in CD3G (gamma+/-) or CD3D (delta+/-, delta+/leaky) with that of normal controls, were compared. PMID:23336327
Altered expression of the TCR signaling related genes CD3 and FcepsilonRIgamma in patients with aplastic anemia. PMID:22401598
A transgenic T cell receptor gammadelta-low expressing subset of T cells accumulates in mouse ear keratinocytes after IL-23 injections. PMID:21984702
report 2 unrelated cases of SCID with a selective block in alphabeta but not in gammadelta T cell development, associated with a new splicing mutation in the CD3D gene PMID:21926461
Data show that the expression pattern of the four CD3 chains was epsilon>zeta>delta>gamma in peripheral blood mononuclear cells from MM, while a gamma>epsilon>zeta>delta expression pattern was found in healthy controls. PMID:21669053
Study characterized the expression pattern of CD3-gamma, -delta, -epsilon and -zeta chain genes from placenta, which contributes to further understanding of the features of T-cell immune status in placenta. PMID:21669059
A combination of trastuzumab antibody and phosphoantigen-stimulated gammadelta T-lymphocytes increases the efficacy of trastuzumab alone against HER-2-positive breast carcinoma cell lines in vivo and mammary carcinoma xenografts in mice. PMID:21670311
Protein sequence comparison indicates that the CD3elta and CD3gamma subunits evolved with highly homologous heterodimeric interfaces and membrane proximal segments for efficient and specific signaling transfer when paired with CD3epsilon. PMID:20660709
When compared with other human T cell subsets, T cell receptor/CD3-activated Vgamma9Vdelta2-expressing T cells display an unusually delayed and sustained intracellular calcium mobilization, dramatically quickened and shortened on costimulation by NKG2D. PMID:20511557
CD3 epsilon undergoes a conformational change after dimerization with CD3 gamma or CD3 delta PMID:12410792
defect in CD3delta gene in severe combined immunodeficiency is characterized by the absence of T cells but normal B cells PMID:14602880
crystal structure at 1.9-A resolution of a complex between a CD3-epsilon/delta ectodomain heterodimer and a single-chain fragment of the UCHT1 antibody PMID:15534202
SCID is caused by a CD3D deficiency. PMID:15546002
a single, membrane-distal YxxO motif in CD3delta could mediate approximately 75% of receptor internalization, whereas its removal only reduced internalization by approximately 20% PMID:15778375
A human CD3 transgene that encodes full length CD3delta and a truncated but functional form of CD3epsilon restored the defective preTCR function in not only CD3epsilon- but CD3gamma- and CD3gammadelta-deficient mice as well. PMID:16412509
CD3delta and CD3gamma play a different role in humans and mice in pre-TCR and TCR function during alphabeta T-cell development PMID:16888097
analysis of TCRalpha-CD3deltaepsilon and TCRbeta-CD3gammaepsilon dimers and the role of the membrane-proximal tetracysteine motif PMID:17023417
The CD3 delta immune recognition receptor cytoplasmic domain binds to acidic and mixed phospholipid vesicles with a binding strength that correlates with the protein net charge and the presence of clustered basic amino acid residues. PMID:17176095
In CD3gamma-deficient patients there can be substitution of CD3gamma by the CD3delta chain and which can then support gammadelta T cell development. PMID:17923503
Enumeration of NK cells and of T lymphocytes expressing TCR alpha/beta in human body effusions is not helpful in attempting to distinguish between benign and malignant effusions. PMID:18803280
Stage-dependent molecular changes in Notch signaling that are critical for normal human T-cell development. PMID:19056690
Data show CD3 epsilon pairs with CD3 gamma or with CD3 delta, forming CD3 epsilon gamma and CD3 epsilon delta heterodimers, which provide insight into our understanding of the molecular assembly of the CD3 molecular complex. PMID:19724882