CASP9 Recombinant Monoclonal Antibody

Code: CSB-RA940979A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IF, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P55211

Target Names

CASP9

Alternative Names

Caspase-9 (CASP-9) (EC 3.4.22.62) (Apoptotic protease Mch-6) (Apoptotic protease-activating factor 3) (APAF-3) (ICE-like apoptotic protease 6) (ICE-LAP6) [Cleaved into: Caspase-9 subunit p35, Caspase-9 subunit p10], CASP9, MCH6

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human CASP9

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8G5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of HepG2 with CSB-RA940979A0HU at 1:7, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 525-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing HepG2 cells stained with CSB-RA940979A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Caspase-9 serves as a critical initiator caspase in the intrinsic apoptotic pathway, functioning as the molecular link between mitochondrial cytochrome c release and downstream executioner caspase activation. Upon apoptotic stimulation, CASP9 is recruited to the apoptosome complex where it undergoes autocatalytic processing into its active p35 and p10 subunits, triggering the proteolytic cascade that commits cells to programmed death. This central position in apoptotic signaling makes CASP9 an essential target for researchers investigating cancer cell survival mechanisms, therapeutic resistance, and metabolic stress responses.

This recombinant rabbit monoclonal antibody, clone 8G5, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human CASP9 and produced through recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your results remain comparable across extended studies. The affinity-purified IgG format delivers clean signal with reduced background interference.

Validation studies demonstrate reliable performance in both immunofluorescence and flow cytometry applications using HepG2 hepatocellular carcinoma cells. Immunofluorescence staining at dilutions from 1:50 to 1:200 reveals clear cytoplasmic localization patterns consistent with CASP9's known subcellular distribution. Flow cytometry analysis confirms specific detection with distinct positive population shifts compared to isotype controls, supporting quantitative assessment of CASP9 expression levels in cell populations.

Whether you are profiling apoptotic pathway activation in cancer models, investigating metabolic regulation of cell death, or characterizing therapeutic responses in human cell systems, this antibody provides a dependable tool for advancing your research into caspase-mediated cellular processes.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Gene References into Functions

CASP9 mutations cause recurrent folate-resistant neural tube defects. PMID:29358613
Study described rare mutations of the apoptosis gene CASP9 identified in neural tube defect cases and demonstrated that the p.Y251C variant impairs the protein's apoptotic function, suggesting it is a loss-of-function variation; also showed that the p.R191G variant inhibited apoptosis under folate-deficient condition, highlighting the effect of gene-environment interactions in this complex disease. PMID:29365368
these results revealed that caspase9 and activated caspase3 predominantly regulates cell apoptosis in human dental pulp stem cells from deciduous teeth. PMID:29845240
Low CASP9 expression is associated with Colorectal Cancer. PMID:29801534
the results of the present study suggest that miR-96-5p, which is frequently upregulated in hepatocellular carcinoma (HCC), inhibits apoptosis by targeting CASP9. Therefore, miR-96-5p may be a potential therapeutic target for HCC PMID:29658604
CASP9 germline mutations may have played a role at least in part to the susceptibility of development of gliomas in a Li-Fraumeni-like family lacking a TP53 germline mutation. PMID:27935156
The caspase 9 level was significantly lower and related with oxidant status in patients with polycystic ovary syndrome, while the circulating levels of caspases 3 and 7 were statistically similar in both PCOS and control groups. PMID:27899026
Results indicate that the apoptotic protease-activating factor 1 (Apaf-1) apoptosome activates caspase-9 in part through sequestration of the inhibitory caspase recruitment domains (CARDs) domain. PMID:28143931
DES1 plays a key role in palmitic acid-mediated caspase 9 and caspase 3 activation. PMID:27364952
CASP9 expression associated with inhibition of miR-182. PMID:28298075
CASP-9 polymorphism is associated with Primary Brain Tumors. PMID:28870924
High CASP9 expression is associated with Lung Tumorigenesis. PMID:27197231
knockdown of HMGI-C led to the significant induction of apoptosis via mitochondrial pathway by inducing miR34a and cell cycle arrest in MDA-MB-468 cells in vitro. PMID:27245202
Results suggest that the formation of the apoptosome accompanied by the activation of caspase-9 may occur in brains affected by multiple system atrophy (MSA), and that a mitochondria-dependent apoptotic pathway may be partially associated with the pathogenesis of MSA. PMID:27345387
Using recombinant proteins, this study investigated the influence of survivin on the inhibition of caspase-9 by XIAP in vitro. With a fluorescence-based assay for the apoptosome-stimulated activity of caspase-9, the study shows that survivin has no effect on the inhibition of caspase-9 by XIAP, neither in the presence nor in the absence of Smac. PMID:27865841
Data show that caspase 9 (CASP9) single nucleotide polymorphism (rs1052576) TT genotype was seemed to be associated with higher risk of pathological stage. PMID:28358701
The content of caspase 9 gene transcripts in peripheral blood leukocytes and plasma level of TNF-alpha were significantly higher in healthy subjects carrying C allele than in carriers of GG genotype. PMID:28091912
Results show that mRNA and protein levels of HAX-1 in prostate cancer cell lines were significantly higher and inhibits cell apoptosis through caspase-9 inactivation. PMID:26323553
Renal CASP9 expression is increased in diabetes and increased as diabetic nephropathy progressed. PMID:27141571
Inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells. PMID:26290316
Levels of caspase-9, caspase-10, MAVS, and pIRF7 in mononuclear cells and the disease activity index (SLEDAI) in the systemic lupus erythematosus patients were determined. PMID:25370148
Expression of mutant caspase-9 correlated with a downregulation of BAFFR (B-cell-activating factor belonging to the TNF family (BAFF) receptor) in B cells and ICOS (inducible T-cell costimulator) in T cells. PMID:25569260
caspase-9 mediates Puma activation to determine the threshold for overcoming chemoresistance in cancer cells. PMID:25356864
we suggest that phosphorylation of caspase-9 may be a useful tool to assess the state of gastrointestinal cancer and the effects of anti-cancer therapy. PMID:25031754
DNA fragmentation, DNA damage, caspase-9 activation and a large increase in the sub-G1 and S cell cycle phases confirmed the occurrence of apoptosis in a time-dependent manner. PMID:24377517
Data indicate a significant association of two single nucleotide polymorphism (SNPs) in aspase9 (CASP9) and two haplotypes of the four SNP combinations with acute myeloid leukemia (AML) susceptibility. PMID:24879622
silica and double-stranded RNA (dsRNA) synergistically induces caspase-9-dependent apoptosis, but not inflammasome activation, of bronchial epithelial cells PMID:24661197
that KAT5 RNAi may result in cleaved casp9 upregulation through p38MAPK activation in Gallbladder carcinoma cells PMID:24427328
overexpression of iASPP and the low expression of caspase-9 in esophageal cancer are closely correlated with tumor invasion and metastasis PMID:24405603
the Atg7.caspase-9 complex performs a dual function of linking caspase-9 to the autophagic process while keeping in check its apoptotic activity. PMID:24362031
The inhibitory effect of Ab42 on the apoptotic pathway is associated with its interaction with procaspase-9 and consequent inhibition of Apaf-1 apoptosome assembly PMID:24424093
The rs4645981 T allele and the rs4645981 T allele carrier might increase the risk of cancer, but the rs1052576 A allele, rs1052576 A carrier, rs2308941 T allele, and rs2308941 T carrier might be protective. PMID:23479167
Interactions of caspase-9 with the BIR3 (baculovirus inhibitory repeat 3) exosite are essential for high-affinity binding. PMID:23203690
In oligozoospermia, spermatogonia presented significant increased active caspase-9 PMID:23359247
The change of caspase-9 expression from colon mucosa, adenoma to cancer suggested it may be involved in the carcinogenesis of colon cancer. PMID:24592539
dCas9 effectors can exert positive or negative regulation on the expression of developmentally relevant genes, which can influence cell differentiation status when impinging on a key node in the regulatory network that governs the cell state. PMID:24346702
BIRC5-31CC and CASP9+83CT/TT genotypes were associated with an increased risk for renal cell carcinoma development in the female group of our southern European study population. PMID:23645041
OSU-03012 induces apoptosis in human esophageal carcinoma cells through a p53/Bax/cytochrome c/caspase-9-dependent pathway. PMID:23652278
EGCG, both alone and in combination with cisplatin, promoted the expression of the pro-apoptotic splice isoform of caspase 9 PMID:23615977
The results demonstrated that 50 microg/mL beta-glucan significantly repressed the expression of the ERCC5 gene, no change in CASP9 expression, and induction of the CYP1A1 gene. PMID:23424205
CASP-9 polymorphisms are associated with susceptibility to low back pain during military training in Chinese soldiers. PMID:23725396
Results found that polymorphisms of CASP9 and CASP10 genes may not contribute to CRC risk in Chinese population. PMID:23303631
Proteolytic processing of the caspase-9 zymogen is required for apoptosome-mediated activation of caspase-9 PMID:23572523
Polymorphism in Caspase 9 (-1263 A>G) was observed to be associated with susceptibility of papillary thyroid carcinoma (PTC). PMID:22120515
Induction of caspase-9b expression is due to activation of hnRNP L via phosphorylation to compete/inhibit hnRNP U association with exon 3 of Casp9 mRNA. PMID:23396972
These results clearly indicated that olive oil phenolic extract and gallic acid were able to inhibit the caspase 9 dependent apoptosis pathway in HeLa cells. PMID:22086301
The overexpression of c-Jun, p73 and Casp-9 in thymic epithelial tumors is closely related with the pathogenesis and biological behavior of the neoplasms. PMID:22974165
Data suggest that CD95L-triggered endogenous ceramide increase in Jurkat leukemia T cells is likely involved in the pro-apoptotic mitochondrial pathway leading to caspase-9 activation. PMID:22306364
caspase-9 is the most important regulator in DLD-1, HCT-116, and HeLa cells PMID:23038270
CASP9 promoter polymorphisms rs4645978 and rs4645981 are associated with breast cancer susceptibility; CASP9 transcriptional regulation is an important factor during breast cancer development PMID:22981751

Hide All

Protein Families

Peptidase C14A family

Tissue Specificity

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

Database Links

HGNC: 1511

UNIGENE: Hs.329502

KEGG: hsa:842

STRING: 9606.ENSP00000330237

OMIM: 602234

Promotions