CALM3/CALM2/CALM1 Recombinant Monoclonal Antibody

Code: CSB-RA829042A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC, FC

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Target Background

Product Details

Uniprot NO.

P0DP23 P0DP24 P0DP25

Target Names

CALM3/CALM2/CALM1

Alternative Names

CALM1 antibody; CALM antibody; CAM antibody; CAM1Calmodulin-1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human CALM3/CALM2/CALM1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4D12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA829042A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing MCF-7 cells stained with CSB-RA829042A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Calmodulin serves as a master regulator of calcium signaling, mediating the effects of intracellular calcium fluctuations on hundreds of downstream target proteins. The three human calmodulin genes—CALM1, CALM2, and CALM3—encode identical 17 kDa proteins, making this calcium sensor one of the most highly conserved proteins across eukaryotes. Given calmodulin's central role in processes ranging from cell cycle progression to neurotransmission, reliable detection of this ubiquitous signaling hub is essential for researchers investigating calcium-dependent pathways in health and disease.

This recombinant rabbit monoclonal antibody, clone 4D12, offers the reproducibility that calcium signaling studies demand. Because the antibody sequence is defined and production occurs in controlled recombinant systems, researchers can expect consistent performance across experiments and over time—eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation data demonstrate this antibody's versatility across complementary detection platforms. Immunohistochemistry performed on paraffin-embedded human colorectal cancer tissue reveals clear target localization using citrate-based antigen retrieval, with effective staining at 1:100 dilution. Flow cytometry analysis of MCF-7 breast cancer cells confirms intracellular detection capability, showing distinct positive signal separation from isotype control when cells are fixed and permeabilized prior to staining.

This combination of validated applications provides researchers with flexibility to examine calmodulin expression at both tissue architecture and single-cell resolution levels. The antibody's demonstrated performance in human cancer specimens and cell lines makes it particularly relevant for oncology research, where dysregulated calcium signaling frequently contributes to tumor progression, as well as broader investigations into signal transduction mechanisms.