CALB2 Recombinant Monoclonal Antibody

Code: CSB-RA193105A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IF, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P22676

Target Names

CALB2

Alternative Names

Calretinin (CR) (29 kDa calbindin), CALB2, CAB29

Species Reactivity

Human

Immunogen

A synthesized peptide from human CALB2 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of SH-SY5Y cell with CSB-RA193105A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA193105A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Calretinin, encoded by the CALB2 gene, is a calcium-binding protein belonging to the EF-hand family that plays essential roles in calcium signaling and neuronal function. This protein serves as a well-established marker for specific neuronal populations, particularly in the central and peripheral nervous systems, and has gained significant importance in diagnostic pathology for identifying mesothelioma and certain neuroendocrine tumors. Its expression pattern makes it a valuable tool for researchers investigating calcium homeostasis, neuronal development, and tumor classification.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human CALB2, offers the consistency and reliability that demanding research applications require. As a recombinant antibody with a defined sequence, it provides exceptional lot-to-lot reproducibility, ensuring that your experimental conditions remain stable across long-term studies and collaborative projects. The rabbit IgG format and affinity-chromatography purification deliver high specificity for detecting calretinin in human samples.

Validation studies demonstrate robust performance in both immunofluorescence and flow cytometry applications using SH-SY5Y neuroblastoma cells, a relevant model for neuronal research. Immunofluorescence staining reveals clear cytoplasmic localization when used at dilutions between 1:50 and 1:200, while flow cytometry analysis shows distinct positive population shifts compared to isotype controls, confirming specific target recognition. These validated applications provide flexibility for researchers working with fixed and permeabilized cell preparations.

This antibody supports investigations into calcium-dependent signaling pathways, neuronal marker expression studies, and cancer research where calretinin serves as a diagnostic indicator. Its demonstrated performance in cellular assays makes it particularly suited for characterizing neuronal cell populations and examining CALB2 expression patterns in relevant disease models.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Gene References into Functions

we have identified septin 7, an essential cellular component implicated in the final steps of cell division, as a strong Bt-dependent gene regulatory protein binding to the promoter of CALB2. PMID:29699512
Calretinin was positive in 20 cases with MM. PMID:27761727
Calretinin could be verified as a blood-based marker for Malignant mesothelioma (MM). The assay is robust and shows a performance that is comparable to that of mesothelin. Retrospective analyses would not be limited by storage time. The high specificity supports a combination of calretinin with other markers. PMID:28558669
calretinin increases Leydig cell viability and proliferation, possibly via ERK1/2 and AKT pathways, and suppresses apoptosis possibly via the mitochondria-related apoptotic pathway PMID:28437248
expression of calretinin in human fetal testis specimens, was examined. PMID:27990638
In Malignant mesothelioma , increased calretinin expression is associated with epithelioid histology and better survival. PMID:27780599
Calretinin, D2-40 and mesothelin are aberrantly expressed in a proportion of CRC cases. PMID:27062033
We studied 38 bone marrow biopsy specimens of non-CML myeloproliferative neoplasms from 31 patients. All eight bone marrow biopsy specimens from patients with mutant CALR showed strong cytoplasmic staining of the megakaryocytes (83.5%; range, 50%-98%; median, 87%) with the CAL2 antibody. PMID:27686170
NCX1 and the calcium binding protein calretinin cooperate within the striatum to confer tolerance against cerebral ischemia. PMID:25633096
Overexpression of human calretinin in mouse primary mesothelial cells inversely affects proliferation and cell migration. PMID:26695618
Patients with Down syndrome may have enteric nervous system disorders functionally similar to HD, related to abnormal or imbalanced autonomic innervation, of which distal calretinin hypoinnervation is one manifestation, despite the presence of ganglia. PMID:26230373
the unique disposition of CALR at the MII spindle uncovers a physical interaction with endoplasmic reticulum that may mediate cytoskeletal remodelling during oocyte maturation. PMID:25620022
In focal cortical dysplasia (FCD), the density of CR positive neurons was increased in cortical layer V compared to non-FCD temporal lobe. PMID:26081613
might be used as a marker for the ameloblastomas and could play an important role in the differentiation of aggressiveness of different odontogenic tumors PMID:25046230
We concluded that WT1, but not calretinin, is a useful prognostic factor in malignant pleural mesothelioma PMID:24323466
This article reviews the information that is currently available on calretinin expression in tumors and on its application as an immunohistochemical marker in diagnostic pathology. PMID:24185118
Calretinin positivity was observed in all rectal biopsies of Hirschsprung disease showing the presence of ganglion cells, and the staining was consistently absent in all cases of aganglionosis. PMID:25119007
Calretinin IHC offered additional diagnostic value in the Hirschprung disease using specimens with inadequate amount of submucosa and rarely seen ganglion cells. PMID:24294384
calretinin immunoexpression and the hormonal status for estrogen receptors (ER), progesterone receptors (PR), and ERRB2, in 33 cases of cutaneous metastases of ductal breast carcinoma, as well as in seven primary cutaneous apocrine tumors, were studied PMID:24322014
Restricted diffusion of calretinin in cerebellar granule cell dendrites implies Ca(2)-dependent interactions via its EF-hand 5 domain. PMID:23732647
Calretinin was found in nerve fibrils in all of the fetal colonic samples. Calretinin was expressed in the gut as early as 22 gestational weeks. PMID:23701779
CD34/calretinin immunohistochemistry could be useful for differentiating sinonasal schwannoma from its histological mimics. PMID:23995573
high-level calretinin expression in invasive ductal carcinoma of the breast is most common in grade 3 tumors with a basal-like phenotype and is associated with poor overall survival. PMID:24262018
Data indicate that fallopian tube and the epithelial component of Brenner tumours (BTs) expressed AKR1C3 and androgen receptor, but the tumour stromal cells showed strong expression of calretinin, inhibin and steroidogenic factor 1 in the majority of BTs. PMID:24012099
All 12 patients without Hirschsprung disease had calretinin-positive nerve fibers in the lamina propria or muscularis mucosae, and all 5 patients with Hirschsprung disease had no calretinin staining of nerves. PMID:23899067
High Calretinin expression is associated with mesothelioma. PMID:23595591
Our results show that different levels of serous ovarian tumor progression are accompanied by changes in the immunohistochemical expression pattern of EpCAM, CD24, and calretinin PMID:23011824
an unexpected role for CR in directly modulating effectors such as Ca(v)2.1, which may have major consequences for Ca(2+) signaling and neuronal excitability. PMID:23033479
This study demonistrated that significant linear regression between calbindin expression and short-term and long-term percent retention of auditory verbal learning test in patients whose epilepsy PMID:22796338
Data show that calretinin and CK5/6 were positive in 100 and 64% of mesotheliomas, and 92 and 31% of reactive effusions, respectively, and desmin was negative in all malignant cases and positive in 85% of reactive effusions. PMID:23075894
Over-expression of CR reduces huntingtin-caused cytotoxicity in both non-neuronal and neuronal cell models of Huntington's disease. PMID:22891683
A set of cryosections revealed extensive colocalization of calretinin with vasoactive intestinal peptide in both surviving submucosal perikarya and mucosal nerve fibres in human chagasic submucosa and mucosa. PMID:22555304
Immunohistochemical study of calretinin expression in normal skin and cutaneous adnexal proliferations. PMID:22343111
Low calretinin is associated with high neutrophil-to-lymphocyte ratio and malignant mesothelioma. PMID:22011651
Calretinin appears to be a useful marker to distinguish Olfactory neuroblastoma from other small round blue cell tumors PMID:22020045
Results suggest that following 5-FU treatment in CRC cell lines, CALB2 is involved in apoptosis induction through the intrinsic mitochondrial pathway. This indicates that CALB2 may be an important mediator of 5-FU-induced cell death PMID:21629658
calretinin-containing cells in the human hippocampus are highly vulnerable, thus inhibition mediated by dendritic inhibitory cells and their synchronization by interneuron-specific interneurons may be impaired in epilepsy. PMID:20576695
Developed a novel and sensitive ELISA for the detection of calretinin in serum, heparin plasma, or EDTA plasma augmented with calcium from mesothelioma patients. PMID:20509881
identification of two elements that act as butyrate-sensitive repressors in all colon cancer cell lines tested PMID:19998412
The overall density of Calretinin neurons in cerebral cortex was significantly decreased in focal cortical dysplasias. PMID:19854615
Calretinin plays a crucial role in the modulation of intrinsic neuronal excitability and the induction of long-term potentiation. PMID:19450707
a tumor marker for cardiac myxoma PMID:11642722
Two types of calretinin-immunostained terminals were found in the CA1 region. PMID:12064772
AP2-like cis element acts as an enhancer of gene expression in neuronal cells PMID:12359331
E-cadherin and calretinin are sensitive and specific in differential diagnosis of benign and malignant serous effusion specimens. PMID:15285029
Disorders of Sertoli cell differentiation as indicated by calretinin and/or cytokeratin-18 expression contribute to the multifactorial mechanisms underlying spermatogenic failure PMID:15950053
Calretinin-immunoreactive unipolar brush cells are presented in the vermis after the 28th gestational week. At birth, they are presentm in the vestibular lobules. At the birth the number of calretinin-immunoreactive unipolar brush cells increase. PMID:16289944
Calretinin immunostaining provides some insights into the histogenesis of invasive and noninvasive implants in serous borderline tumors of the ovary. PMID:16415795
Cells lining the mesothelial inclusion cysts had CAL2 immunohistochemical reactivity. PMID:17535091
The combinational characterization of calretinin (either by polyclonal or monoclonal antibody), inhibin alpha, and Melan-A expression is of great significance in the differential diagnosis of adrenocortical tumors. PMID:18091323