BIRC5 Recombinant Monoclonal Antibody

Code: CSB-RA209144A0HU

Rate （5.0）
Review （1）
Citation （1）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

O15392

Target Names

BIRC5

Alternative Names

Baculoviral IAP repeat-containing protein 5 (Apoptosis inhibitor 4) (Apoptosis inhibitor survivin), BIRC5, API4 IAP4

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Survivin

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6G11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA209144A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Description

BIRC5, commonly known as Survivin, represents one of the most compelling targets in cancer biology research. As a member of the inhibitor of apoptosis protein family, Survivin plays a dual role in suppressing programmed cell death while regulating cell division through its association with the chromosomal passenger complex. Its expression pattern makes it particularly valuable for oncology studies—while largely absent in most normal adult tissues, Survivin becomes dramatically upregulated across numerous tumor types, positioning it as both a diagnostic marker and therapeutic target of significant interest.

This recombinant monoclonal antibody, clone 6G11, offers the reproducibility that demanding research protocols require. Generated against a synthetic peptide derived from human Survivin and produced using recombinant technology in rabbit host, this antibody delivers the sequence-defined consistency that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. Researchers can confidently build upon previous results knowing that antibody performance remains stable across extended studies.

Validation in immunohistochemistry demonstrates reliable detection in human tissue samples. Testing in paraffin-embedded human tonsil tissue using a Leica Bond system with citrate buffer antigen retrieval at pH 6.0 confirms specific staining at dilutions ranging from 1:50 to 1:200, providing flexibility to optimize signal intensity for your particular tissue preparations. The antibody is additionally validated for ELISA applications.

For investigators exploring apoptosis regulation, cell cycle control, or tumor biology, this antibody supports research spanning neuroscience, cancer biology, and fundamental cell biology applications. The unconjugated format allows pairing with your preferred detection system for maximum experimental flexibility.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : WB

Sample type: Human Cell

Review: Western bolt assays showed that the protein levels of Birc5 was promoted after shRNA-LTBP4-KD11 or KD2 was transfected into SK-MEL-1 and VMM5A cells. Data are presented as the mean ± standard deviation. ** P< 0.01

By Anonymous

Citations

LTBP4 Inhibits the Proliferation and Metastasis in Melanoma by Activating Hippo-YAP Signaling L na Wang,/,2020

Target Background

Function(From Uniprot)

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Essential for the maintenance of mitochondrial integrity and function. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Gene References into Functions

NF-kappaB activation may infer resistance to apoptosis through the expression of anti-apoptotic genes such as Survivin, which showed progressive increase in expression throughout the oesophageal metaplasia-dysplasia-adenocarcinoma sequence. PMID:29473241
Study demonstrate that ZIC1 plays a tumor suppressive role in breast cancer, by targeting surviving, significantly downregulating its expression. PMID:29956756
Studying survivin expression in leukocytes of 144 female rheumatoid arthritis patients this study observed that smoking patients had higher survivin transcription and a remarkable spreading of survivin isoforms. PMID:27915033
Our findings identify survivin as a target of HO-1 and a mediator of adipocyte-induced survival in the metastatic niche. PMID:29311669
High BIRC5 expression is associated with gefitinib resistance in lung cancer. PMID:30106446
miR-203 expression also inhibited primary tumor growth in ovaries and metastatic tumors in multiple peritoneal organs including liver and spleen. miR-203 inhibits ovarian tumor metastasis by targeting BIRC5/survivin and attenuating the TGFbeta pathway. PMID:30241553
Survivin may be implicated in the bcl-2 and p53 pathways and therefore in the biology of PDAC. Its potential use as a survival predictor and therapeutic target represent a promising field. PMID:30249893
Study in hepatocellular carcinoma cell line elicited a new mechanism in which IGF-1 induced epithelial-mesenchymal transition through regulation of survivin and a downstream pathway. PMID:29989646
In the present study, liposomeplasmid DNA encoding mutant survivinT34A could inhibit tumor growth of cervical cancer. This inhibition may be associated with an increase in the apoptosis rate of tumor cells and a reduction in angiogenesis. PMID:29767242
simvastatin significantly inhibited the proliferation and invasion of SACC83 cells, induced apoptosis, and reduced the expression of survivin, which suggests that simvastatin may be a novel target for salivary gland adenoid cystic carcinoma therapy. PMID:29956779
Survivin is significantly up-regulated in hepatocellular carcinoma (HCC)tissues and associated with tumor growth and lymph node metastasis. Clinical detection of survivin level combined with MRI examination might be beneficial for clinical diagnosis and treatment of HCC PMID:30010107
Our study identified the STAT3 rs1053004 C/C as a high-risk genotype in MA with lower survivin and VEGF transcription levels in the peripheral blood. PMID:30226700
Overexpression of miR-485-5p suppresses breast cancer progression and enhances chemosensitivity. Further study demonstrated that miR-485-5p directly targeted the 3'-untranslated region of survivin and overexpression of survivin overcomes the miR-485-5p induced effects on breast cancer. PMID:29678577
Survivin expression in gastric cancer cells is regulated by DEC1. PMID:29204860
High serum survivin levels with GG genotype are associated with Brain Tumors. PMID:30275230
LNC473 could recruit deubiquitinase USP9X to inhibit the ubiquitination level of survivin and then increase survivin expression. PMID:29605299
Oct4 plays a vital role in the malignant progression of HCC cells through the survivin/STAT3 signaling pathway. PMID:29901157
Our study results may suggest that high serum survivin levels can show 4 times increased risk of cancer in a subject with a high suspicion of cancer. Furthermore, survivin level was not influenced with demographic characteristics of breast, gastric, colorectal, prostate, ovarian cancer, and glioblastome multiforme. PMID:29893319
These findings collectively suggest that the triple combination of survivin knockdown with ABT-263 and trametinib treatment, may be a potential strategy for the treatment of KRAS-mutant lung adenocarcinoma. Furthermore, our findings indicate that the welldifferentiated type of KRAS-mutant lung tumors depends, at least in part, on TTF1 for growth. PMID:29658609
Targeting the Cripto-1/TAK-1/NF-kappaB/Survivin pathway may be an effective approach to combat apoptosis resistance in cancer. PMID:29807222
suggests SIRT1 may serve as a predictor of poor prognosis in esophageal squamous cell carcinoma, and its mediated tumor-promoting role might be associated with the overexpression of EGFR protein in esophageal squamous cell carcinoma PMID:29625788
CSN5 directly bound survivin and decreased its ubiquitination to enhance the protein stability of survivin. PMID:29596838
The survivin gene 3' UTR polymorphisms (rs17878624) show that GG genotype provides substantial protection from non-small-cell lung carcinoma. PMID:29631694
Concomitant high expression of survivin and VEGF-C is closely associated with LNM status of PTC patients, which suggests their cooperation in the metastatic process. PMID:29578160
In leukoplakia, the expression of survivin associated with that of ki-67 reinforces the assumption that all these lesions are potentially malignant. PMID:28346726
Study showed for the first time that the suppression of rheumatiod arthritis fibroblast-like synoviocyte was mediated by phosphatase and tensin homolog involving survivin silencing. PMID:28337018
ERCC1 expression may also inhibit esophageal squamous cell carcinoma cell apoptosis via regulating survivin expression, and ERCC1 and survivin overexpression are independent predictors of prognosis for ESCC patients who receive chemotherapy and/or radiotherapy PMID:30075571
let-7b targets PLK1 to inhibit hepatocellular carcinoma cell growth and induce their apoptosis by attenuating the PLK1-mediated Survivin phosphorylation PMID:29913237
Overexpression of Survivin in glioma cells induces chromosomal instability PMID:29282022
High BIRC5 expression is associated with ovarian cancer. PMID:29795564
In III-rd trimester of pregnancy parameters of Timp-1 and Survivin - anti-apoptotic substances concentration were similar in maternal and cord blood in both artery and vein. We found no increased activity of selected antiapoptotic factors. PMID:28509321
Results form study in non-small-cell lung cancer cells showed that SphK2 plays a critical role in doxorubicin-induced resistance by regulating key anti-apoptotic gene, survivin. PMID:28950390
Survivin overexpression plays a key role in the chemoresistance of ovarian CSCs. PMID:30061219
Survivin may play an important role in the occurrence and development of laryngeal carcinoma, and its high expression is related to the poor prognosis of patients with laryngeal cancer. (Meta-analysis) PMID:29270761
treatment of DLD1 cells with tamoxifen , betaestradiol, or a combination of these two drugs, inhibited cell viability and migration, promoted cell apoptosis, and reduced the mRNA and protein expression levels of survivin in a dose and timedependent manner. These results provide novel experimental basis for hormonal adjuvant therapy for the treatment of colorectal cancers PMID:28849238
Studies indicated that high survivin expression in renal cell carcinoma (RCC) was associated with poor overall survival [Review]. PMID:27411378
Nuclear accumulation of survivin is associated with proliferative phenotype and was shown to be a worse prognostic marker in breast ductal carcinoma. PMID:29517199
Knockdown of BIRC5, a member of the inhibitor of apoptosis protein family, using either lentiviral vector based CRISPR/Cas9 nickase gene editing or inhibition of survivin using the small-molecule inhibitor YM155, results in the suppression of epithelial to mesenchymal transition in retinal pigment epithelial cells. PMID:29522718
By downregulation of Sp1 and survivin at the late phase of treatment. PMID:28713892
The expressions of survivin and STAT2 are up-regulated in skin lesions of PV patients, and their mRNA expressions are positively correlated. PMID:29089085
Findings suggest that lysosome-associated transmembrane protein 4B (LAPTM4B), vascular endothelial growth factor (VEGF), and nuclear survivin expression are significantly correlated in breast cancer, which may be predictive of prognosis as well as effective therapeutic targets for anticancer therapies. PMID:28476037
HIF-2alpha dictates the resistance of human pancreatic cancer cells to TRAIL under normoxic and hypoxic conditions and transcriptionally regulates survivin expression. PMID:28476028
survivin may have a role in recurrence in rectal cancer patients treated with surgery and postoperative concurrent chemo-radiation therapy PMID:27391438
Results identified BIRC5 to be significantly upregulated in the lung squamous cell carcinoma tissues of smoking patients and may play an important role in diagnosis and prognosis. PMID:28949095
After cancer cell fusion, some fused cells avoid the apoptotic crisis partly owing to survivin, and continue to proliferate, a process that contributes to human cancer progression. PMID:28193315
High survivin expression is associated with lung cancer and colorectal cancer. PMID:27602754
Data suggest that Ki-67 index and survivin may be useful biomarkers for rectal cancer with preoperative chemoradiotherapy. PMID:29491110
inhibition of apoptosis targeting survivin might represent an effective strategy for both obesity and cancer therapy. PMID:28518147
FAT10 promotes tumor proliferation by directly stabilizing Survivin protein in breast cancer cells. PMID:27806337
nuclear survivin is a prognostic marker for the progression of oral squamous cell carcinomas. PMID:28384094

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Subcellular Location

Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody.

Protein Families

IAP family

Tissue Specificity

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. Ex

Database Links

HGNC: 593

UNIGENE: Hs.744872

KEGG: hsa:332

OMIM: 603352

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