BIRC3 Recombinant Monoclonal Antibody

Code: CSB-RA037080A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, FC

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Target Background

Product Details

Uniprot NO.

Q13489

Target Names

BIRC3

Alternative Names

Baculoviral IAP repeat-containing protein 3 (EC 2.3.2.27) (Apoptosis inhibitor 2) (API2) (Cellular inhibitor of apoptosis 2) (C-IAP2) (IAP homolog C) (Inhibitor of apoptosis protein 1) (hIAP-1) (hIAP1) (RING finger protein 49) (RING-type E3 ubiquitin transferase BIRC3) (TNFR2-TRAF-signaling complex protein 1), BIRC3, API2 MIHC RNF49

Species Reactivity

Human

Immunogen

Recombinant Antibody

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7E9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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Western Blot
Positive WB detected in: Raji whole cell lysate(30µg)
All lanes: BIRC3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 68 kDa
Observed band size: 72 kDa
Exposure time：3min
Overlay Peak curve showing Raji cells stained with CSB-RA037080A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

BIRC3, also known as cellular inhibitor of apoptosis 2 (c-IAP2), plays a critical role in regulating programmed cell death and NF-κB signaling pathways. As a RING-type E3 ubiquitin ligase, BIRC3 modulates cell survival decisions by inhibiting caspases and facilitating ubiquitin-dependent signaling downstream of TNF receptor complexes. Its dysregulation has been implicated in various malignancies and inflammatory conditions, making it a significant target for researchers investigating apoptotic mechanisms, cancer biology, and immune signaling networks.

This recombinant rabbit monoclonal antibody (clone 7E9) offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human BIRC3, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can compromise longitudinal studies. Affinity chromatography purification delivers a reagent suitable for quantitative applications where signal reliability matters.

Validation studies confirm robust performance in flow cytometry applications, with recommended dilutions of 1:50 to 1:200. Flow cytometric analysis using Raji cells demonstrated clear positive signal separation from isotype controls, with intracellular staining performed following fixation and permeabilization. Western blot analysis in Raji whole cell lysate detected a band at approximately 72 kDa, slightly higher than the predicted 68 kDa molecular weight—a shift commonly attributable to post-translational modifications such as ubiquitination or phosphorylation events consistent with BIRC3's regulatory functions.

This antibody serves researchers studying apoptosis regulation, NF-κB pathway dynamics, and ubiquitin-mediated signaling in human samples, providing a reliable tool for both cell-based assays and protein detection applications.

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Target Background

Function(From Uniprot)

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8.

Gene References into Functions

High BIRC3 expression is associated with Oral squamous cell carcinoma. PMID:29286141
Results indicate that Pellino-1 contributes to lung oncogenesis through the overexpression of inhibitor of apoptosis protein 2 (cIAP2) and promotion of cell survival and chemoresistance. PMID:27248820
We show that BIRC3 has a unique role in facilitating glioma progression from low- to high-grade PMID:27074575
CIAP2 expression was elevated in human GBC tissues. PMID:28295868
destabilization of TRAF2 by miR-17 reduced the ability of TRAF2 to associate with cIAP2, resulting in the downregulation of TNF-alpha-induced NF-kappaBp65, c-Jun, and STAT3 nuclear translocation and the production of IL-6, IL-8, MMP-1, and MMP-13 in human rheumatoid arthritis synovial fibroblasts. PMID:27534557
The expression of cIAP2 mRNA was significantly higher in the groups with Helicobacter pylori(+), atrophic gastritis/intestinal metaplasia(+), and Helicobacter pylori-positive early gastric cancer than in the control, Helicobacter pylori(+), and atrophic gastritis/intestinal metaplasia(-) groups. PMID:27282269
Polymorphisms in the BIRC3 gene are associated with a protective effect with regards to asthma susceptibility, and a reduced load of inflammatory cells. PMID:27304223
we indicated that miR-34a can inhibit tumor invasion and metastasis in osteosarcoma, and its mechanism may be partly related to downregulating the expression of C-IAP2 and Bcl-2 protein directly or indirectly. PMID:28635396
Study demonstrate that BIRC3 expression increases secondary to acquisition of temozolomide TMZ and irradiation resistance in glioblastoma. PMID:26888114
High BIRC3 expression is associated with pancreatic cancer. PMID:26815504
Network analysis identified BIRC3 as pathogenic gene in childhood asthma. PMID:27420950
The clinical impact of small subclones harboring NOTCH1, SF3B1, or BIRC3 mutations in chronic lymphocytic leukemia patients appears to be less pronounced than that of small TP53 mutated subclones. PMID:26819056
cIAP2 is upregulated in regenerative epithelial cells both in ulcerative colitis and in experimental intestinal wounds. Inhibition of cIAP2 decreases wound healing in vitro possibly through inhibition of migration. [review] PMID:26513451
LapR cells possessed increased levels of 2 of the inhibitors of apoptosis (IAPs), survivin and c-IAP-2, which are reported to block caspase activation downstream of cytosolic cytochrome C release. PMID:25692408
API2-MALT1 induces paracaspase-mediated cleavage of the tumour suppressor protein LIMA1. PMID:25569716
this study candidates BIRC3 as one of the potentially useful predictive marker for discriminating patients with oesophageal and oesophagogastric junction adenocarcinoma who will most likely benefit from preoperative chemoradiotherapy. PMID:26291056
these results indicate that N. gonorrhoeae stimulation of human endocervical epithelial cells induces the release of cIAP2, an essential regulator of cell death and immune signaling. PMID:26077759
Cytoplasmic expression of HuR is associated with cIAP2 expression in OSCCs PMID:23852810
in 11q-deleted patients treated with first-line chemotherapy, ATM mutation rather than BIRC3 deletion and/or mutation identifies a subgroup with a poorer outcome. PMID:24584352
a number of genes not previously known to be affected by RUNX2 expression, in particular BIRC3, genes encoded on the mitochondrial genome, and several genes involved in bone and tooth formation. PMID:24349465
TNF-alpha induced apoptosis of gastric cancer cells via accelerated degradation of the inhibitor of apoptosis family members, CIAP2, XIAP, and survivin. PMID:25513960
cIAP2 is in conclusion a regulator of human intestinal wound healing through enhanced migration along with activation of Rac1 PMID:25394657
Data indicate that tumor necrosis factor inducible protein A20-mediated inhibitor of apoptosis protein-2 (cIAP-2) is important in endothelial cell resistance to tumor necrosis factor alpha (TNF-alpha)-induced apoptosis. PMID:24595242
Identification and function of the NIK IAP binding motif, which promotes c-IAP1-dependent ubiquitylation of NIK. PMID:25246529
Distinctive role of c-IAP2 as stabilizer of XIAP, which is likely involved in regulation of NFkappaB activation and apoptosis in GBM cells was determined. PMID:24552816
increased expression with progression of chronic myeloid leukaemia PMID:24266799
our study implicates RIP1 ubiquitination as a critical component of API2-MALT1-dependent lymphomagenesis PMID:23770847
BIRC3 gene mutation is associated with chronic lymphocytic leukemia. PMID:23558524
we present cIAP-2 as a novel inducer of platinum resistance in ovarian carcinoma cells, and suggest an axis beginning with an encounter between cisplatin and these cells, mediated sequentially by IL-6 and cIAP-2, resulting in cisplatin resistance PMID:23052480
The expression of C-IAP2 in hepatocellular carcinoma is associated with tumor recurrence and metastasis. PMID:22820591
Clonal evolution from lower to higher risk implicated the emergence of NOTCH1, SF3B1, and BIRC3 abnormalities in addition to TP53 and 11q22-q23 lesions in chronic lymphocytic leukemia. PMID:23243274
A novel role for cIAP2 in maintaining wild-type p53 levels by preventing both an NFkappaB-mediated increase and IKKalpha/-beta-dependent transcriptional and post-translational modifications of MDM2. PMID:23032264
study shows API2-MALT1 fusion oncoprotein induces proteolytic cleavage of NF-kappaB-inducing kinase (NIK); resulting deregulated NIK activity is associated with constitutive noncanonical NF-kappaB signaling, enhanced B cell adhesion and apoptosis resistance PMID:21273489
TRAF2 and cIAP2 are involved in TWEAK-induced MMP-9 production in fibroblast-like synoviocytes in rheumatoid arthritis. PMID:21229359
NF-kappaappaB, acting through two response elements, is required for estrogen receptor recruitment to an adjacent estrogen response element in the BIRC3 promoter. PMID:22083956
BIRC3 disruption is a common mechanism across marginal zone B-cell lymphomagenesis. PMID:21881048
our results suggest that CpG-induced protection may be mediated by c-IAP-2 through the calcium-activated JNK pathway PMID:22068233
cIAP2 may play an important role in Helicobacter pylori -induced gastric carcinogenesis PMID:21963223
we confirm that mRNA and protein levels for cIAP2 are highly up-regulated in breast cancer cells by E2 and cytokines PMID:21152357
These data reveal a novel mechanism for the inhibition of hepatitis B virus replication by cIAP2 via acceleration of the ubiquitin-proteasome-mediated decay of polymerase. PMID:21865390
API2-MALT1 fusion gene is a distinctive genetic aberration in MALT lymphomas, and is not present in diffuse large B cell lymphoma. PMID:20079017
Adenosine downregulated the expression of mRNAs and proteins for Bcl-X(L) and inhibitor of apoptosis protein 2 (IAP2) to directly inhibit caspase-3, -7, and -9, but upregulated the expression of mRNA and protein for DIABLO, an inhibitor of IAPs. PMID:20063052
Arsenic trioxide suppresses transcription of cIAP2 mRNA in NB-4 cells. PMID:19763917
These results support a role for Tax-dependent cIAP-2 expression in preventing the death of naturally infected CD8(+) cells and thereby in their clonal expansion in vivo. PMID:20863547
TTP can bind to the 2nd AU-rich elements of cIAP2 mRNA 3'UTR and destabilize cIAP2 mRNA by forming complexes with Dcp2 and Xrn1. PMID:20691152
Poly(I:C) induces intense expression of c-IAP2 and cooperates with an IAP inhibitor in induction of apoptosis in cancer cells. PMID:20576118
cancer cell lines evade Smac mimetic-induced apoptosis by up-regulation of cIAP2, which although initially degraded, rebounds. cIAP2 is induced by TNFalpha via NF-kappaB and modulation of the NF-kappaB signal renders cells sensitive to Smac mimetics PMID:20547836
crystal structures of the TRAF2: cIAP2 and the TRAF1: TRAF2: cIAP2 complexes; biochemical, structural, and cell biological studies on the interaction between TRAF2 and cIAP2 and on the ability of TRAF1 to modulate this interaction PMID:20385093
Results provide the first structures of BIR domains from human NAIP and cIAP2. PMID:19923725
REVIEW: Genetic alterations involving API2 underlying the pathogenesis of MALT lymphoma PMID:11960389

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

IAP family

Tissue Specificity

Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.

Database Links

HGNC: 591

UNIGENE: Hs.127799

KEGG: hsa:330

STRING: 9606.ENSP00000263464

OMIM: 601721

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