BIN1 Recombinant Monoclonal Antibody

Code: CSB-RA231223A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, FC

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Target Background

Product Details

Uniprot NO.

O00499

Target Names

BIN1

Alternative Names

AMPH 2 antibody; AMPH2 antibody; Amphiphysin 2 antibody; Amphiphysin II antibody; Amphiphysin like protein antibody; amphiphysin-like antibody; Amphiphysin-like protein antibody; AMPHL antibody; Bin1 antibody; BIN1_HUMAN antibody; Box Dependant MYC Interacting Protein 1 antibody; Box-dependent myc-interacting protein 1 antibody; Bridging integrator 1 antibody; DKFZp547F068 antibody; MGC10367 antibody; MGC105358 antibody; Myc box dependent interacting protein 1 antibody; Myc box-dependent-interacting protein 1 antibody; SH3P9 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human BIN1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13C4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate(20µg)
All lanes: BIN1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 65 kDa
Observed band size: 65-88 kDa
Exposure time：2min
Overlay Peak curve showing HepG2 cells stained with CSB-RA231223A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

BIN1, also known as Bridging Integrator 1 or Amphiphysin 2, plays a critical role in membrane dynamics, endocytosis, and tumor suppression through its interaction with the MYC oncoprotein. This multifunctional adapter protein has garnered significant research attention due to its involvement in diverse cellular processes, including its established links to Alzheimer's disease susceptibility and its function as a negative regulator of cell proliferation. Understanding BIN1 expression and localization provides valuable insights into membrane remodeling pathways and cancer biology.

This recombinant monoclonal antibody, clone 13C4, offers the reproducibility and consistency that demanding research applications require. Developed using recombinant technology in a rabbit host, this antibody provides sequence-defined specificity against human BIN1, ensuring reliable performance across experimental replicates and eliminating the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies.

Validation studies demonstrate robust performance across multiple platforms. In Western blot applications, this antibody detects BIN1 in HeLa whole cell lysates at dilutions ranging from 1:500 to 1:2000, revealing bands between 65 and 88 kDa. The observed molecular weight range above the predicted 65 kDa likely reflects post-translational modifications, particularly glycosylation, and the presence of multiple BIN1 splice variants known to exist in human cells. Flow cytometry validation in HepG2 cells confirms reliable intracellular detection, with clear separation from isotype control, supporting studies examining BIN1 expression at the single-cell level.

This antibody serves researchers investigating membrane trafficking, MYC-dependent transcriptional regulation, neurodegeneration mechanisms, and tumor suppressor pathways, providing a dependable tool for advancing understanding of this biologically significant protein.

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Target Background

Function(From Uniprot)

Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Is a negative regulator of endocytosis. Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production. In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates. May be involved in the regulation of MYC activity and the control cell proliferation. Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro.

Gene References into Functions

Meta-analysis validated the association of late onset Alzheimer disease with BIN1 (rs744373) variants. PMID:29504051
The findings reveal the ability of Bin1 to modify actin dynamics and provide a possible mechanistic connection between Bin1 and tau-induced pathobiological changes of the actin cytoskeleton. PMID:28893863
the association between the rs744373 polymorphism of BIN1 protein and late-onset Alzheimer's disease in East Asian, American, and European populations (Meta-Analysis) PMID:26846281
Italian family with centronuclear myopathy, carrying a novel pathogenic mutation of BIN1 gene in heterozygous state, consistent with autosomal dominant inheritance. PMID:27854204
bridging integrator 1 Gene rs7561528 polymorphism contributes to Alzheimer's disease susceptibility in East Asian and Caucasian populations PMID:28302384
Findings indicated that BIN1 restoration in NSCLC could reverse PD-L1-mediated immune escape by inactivating the c-MYC and EGFR/mitogen-activated protein kinase pathways. PMID:28714960
propose that efforts to define how genetic variants in BIN1 elevate the risk for Alzheimer's disease would behoove to consider BIN1 function in the context of its main expression in mature oligodendrocytes PMID:27538496
The results emphasize an additional level of complexity in the regulation of the interaction between BIN1 and Tau dependent on the BIN1 isoforms. PMID:28755476
Data (including data from studies using transgenic mice) suggest that the process leading to microparticle release from cardiac myocytes involves recruitment of CHMP4B protein to the forming microparticle membrane which also contains cBIN1; plasma cBIN1 is reduced in patients with heart failure as compared to control subjects. (CHMP4B = charged multivesicular body protein 4B; cBIN1 = cardiac bridging integrator 1) PMID:28806752
Low Bin1 expression is associated with esophageal squamous cell carcinoma. PMID:28152502
the depletion of BIN1 increases cellular BACE1 levels through impaired endosomal trafficking and reduces BACE1 lysosomal degradation, resulting in increased Ab production. Our findings provide a mechanistic role of BIN1 in the pathogenesis of Alzheimer disease (AD), as a novel genetic regulator of BACE1 levels and Ab production PMID:27179792
BIN1 protein expression in cerebral cortex was related to disease progression in Alzheimer's Disease patients. PMID:27346750
data show that the previously described consensus sequence PXRPXR for amphiphysin SH3 ligands is inaccurate and instead define it as an extended Class II binding motif PXXPXRpXR, where additional positive charges between the two constant arginine residues can give rise to extraordinary high SH3 binding affinity. PMID:27268056
findings support a contribution of BIN1 to individual differences in episodic memory performance among Type 2 Diabetes patients. PMID:26947052
analysis of a novel deregulated mechanism in chronic myeloid leukemia patients, indicating BIN1 and RIN1 as players in the maintenance of the abnormal RTK signaling in this hematological disease PMID:26194865
BIN1 is involved with late onset Alzheimer's disease. [review] PMID:27773727
This study supported that BIN1 contributes to the risk of Alzheimer's disease by altering neural degeneration (abnormal tau, brain atrophy and glucose metabolism) but not Abeta pathology PMID:27003210
Alterations in the BIN1 locus, previously associated with Alzheimer disease, may modify the age of onset of GBA-associated Parkinson. PMID:26233692
no association was found for either polymorphism, suggesting that these genes are not implicated in the aetiology of Alzheimer's disease in all populations. PMID:26733302
Data demonstrate that EHBP1L1 links Rab8 and the Bin1-dynamin complex, which generates membrane curvature and excises the vesicle at the endocytic recycling compartment for apical transport. PMID:26833786
In vitro studies in human Caco-2 cells showed that Bin1 antibody altered the expression of tight junction proteins and improved barrier function. PMID:26195312
This study demonistrated that BIN1 mutation releated to Centronuclear myopathy. PMID:25957634
Results show low expression of Bin1, along with high expression of IDO, are predictor for poor prognosis in esophageal squamous cell cancer and thereby could be used to establish new therapeutic strategies. PMID:25683635
The frequencies of BIN1 alleles were similar in both control and Alzheimer patients showing o no association. PMID:26738348
This study findings demonstrate that rs744373 itself or a variation in linkage disequilibrium may provide a neurogenetic mechanism for BIN.1 PMID:25630570
The study is the first to confirm the association of the variant rs7561528 adjacent to Bin1 with Sporadic Alzheimer's Disease in a Han Chinese Population. PMID:25461955
These findings suggest that an intracellular form CLU and BIN1 interaction might impact Tau function in neurons and uncover potential new mechanisms underlying the etiology of Tau pathology in in Alzheimer's disease. PMID:25051234
Reduced BIN1 expression is associated with cutaneous T-cell lymphoma. PMID:25578476
BIN1 rs744373 polymorphism is significantly associated with late onset Alzheimer disease. PMID:25022885
BIN1/M-Amphiphysin2 has a role in inducing clustering of phosphoinositides to recruit its downstream partner dynamin PMID:25487648
The release of BIN1 from hypo-poly(ADP-ribosyl)ated E2F1 is a mechanism by which serum starvation promotes E2F1-induced apoptosis. PMID:25257171
Results suggest that BIN1 is likely involved in Alzheimer's disease as a modulator of neurofibrillary tangle pathology, and that this role may extend to other human diseases that feature tau pathology PMID:25024306
These results support a role for N-WASP in amphiphysin-2-dependent nuclear positioning and triad organization and in centronuclear myopathy and myotonic dystrophy pathophysiology. PMID:25262827
specific amphiphysin 2 mutations can cause either recessive or dominant centronuclear myopathy and that both disorders involve different pathomechanisms PMID:25260562
Brain DNA methylation in BIN1 was associated with pathological Alzheimer disease. PMID:25365775
physical activity attenuated the effects of genetic risk (ie. the constellation of PICALM, BIN1, and CLU polymorphisms) on episodic memory PMID:24660791
The study compares the binding dynamics and affinities of the relevant regions for binding of c-Myc and NS5A to Bin1 SH3. The result gives further insights into the potential role of NS5A in Bin1-mediated apoptosis. PMID:24616074
These results suggest an autoinhibition model for BIN1 that involves a synergistic regulation by membrane composition and protein-protein interactions. PMID:25350771
Two mutants of BIN1 showed impaired membrane tubulation both in vivo and in vitro, and displayed characteristically different behaviors. PMID:24755653
BIN1 downregulation is linked to cancer progression and also correlates with ventricular cardiomyopathy and arrhythmia preceding heart failure. Increased BIN1 expression is linked to increased susceptibility for late-onset Alzheimer's disease. [review] PMID:24590001
Our analyses suggest that these DNA methylation changes may have a role in the onset of Alzheimer disease given that we observed them in presymptomatic subjects and that six of the validated genes connect to a known susceptibility gene network. PMID:25129075
It is the most important genetic susceptibility locus in late-onset Alzheimer's disease. PMID:24582639
BIN1 expression is increased in Alzheimer Disease brains when compared with controls. PMID:23399914
BIN1 is decreased in sporadic but not familial Alzheimer's disease or in aging PMID:24205320
REVIEW--BIN1: form, function, and Alzheimer's disease PMID:23871436
The resukts of this study highlight the possible use of plasma BIN1 as a biomarker for AD diagnosis. PMID:23803295
To our knowledge, this is the first study to show significant association between rs744373 polymorphism and AD in East Asian population. PMID:23570733
Our data demonstrate that the alteration of the muscle-specific function of BIN1 is a common pathomechanism for centronuclear myopathy, myotonic dystrophy, and IMGD. PMID:23754947
We identified rare small events overlapping CR1 and BIN1 in Alzheimer's disease and normal controls with opposite copy number variation dosage. PMID:23202439
The re-expression of BIN1 specifically compromises the proliferation of SNF5-deficient rhabdoid tumors cell lines. PMID:22544318

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Subcellular Location

[Isoform BIN1]: Nucleus. Cytoplasm. Endosome. Cell membrane, sarcolemma, T-tubule.; [Isoform IIA]: Cytoplasm.

Tissue Specificity

Ubiquitous. Highest expression in the brain and muscle. Expressed in oligodendrocytes. Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter. Isoform BIN1 is widely expressed with highest expressi

Database Links

HGNC: 1052

UNIGENE: Hs.193163

KEGG: hsa:274

STRING: 9606.ENSP00000316779

OMIM: 255200

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