BAG1 Recombinant Monoclonal Antibody

Code: CSB-RA981258A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q99933

Target Names

BAG1

Alternative Names

Bag 1 antibody; BAG family molecular chaperone regulator 1 antibody; BAG-1 antibody; BAG1 antibody; BAG1_HUMAN antibody; BCL 2 Associated Athanogene 1 antibody; BCL 2 associated athanogene antibody; BCL 2 binding athanogene 1 antibody; Bcl-2-associated athanogene 1 antibody; BCL2 associated athanogene 1 antibody; BCL2 associated athanogene antibody; Glucocorticoid receptor-associated protein RAP46 antibody; HAP1 antibody; Haptoglobin antibody; RAP 46 antibody; RAP46 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human BAG1 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16C12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), THP-1 whole cell lysate(30µg)
All lanes:BAG1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 39 kDa
Observed band size: 35, 46 kDa
Exposure time：2min
IHC image of CSB-RA981258A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HeLa cell with CSB-RA981258A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with CSB-RA981258A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

BAG1, or Bcl-2-associated athanogene 1, functions as a multifunctional regulator that bridges molecular chaperone activity with apoptotic signaling pathways. This co-chaperone interacts with heat shock proteins and modulates the anti-apoptotic function of Bcl-2, positioning it as a significant target in cancer biology and cellular stress response research. BAG1 also associates with nuclear hormone receptors, including the glucocorticoid receptor, expanding its relevance to studies of hormone signaling and transcriptional regulation.

This recombinant monoclonal antibody, clone 16C12, offers the reproducibility that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent to traditional hybridoma-derived antibodies, ensuring consistent results across extended studies and multi-site collaborations.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis in HeLa and THP-1 whole cell lysates reveals bands at approximately 35 and 46 kDa, which differ from the predicted 39 kDa molecular weight. This pattern likely reflects the known isoforms of BAG1 generated through alternative translation initiation, as well as potential post-translational modifications. Immunohistochemistry performed on paraffin-embedded human colorectal cancer tissue shows clear target detection, while immunofluorescence staining in HeLa cells confirms utility for subcellular localization studies. Flow cytometry validation, also conducted in HeLa cells, demonstrates suitability for quantitative single-cell analysis.

Supplied in a glycerol-containing buffer optimized for long-term stability, this antibody supports researchers investigating apoptosis regulation, chaperone biology, and cancer-related pathways where BAG1 expression or localization may influence disease progression or therapeutic response.

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Target Background

Function(From Uniprot)

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.

Gene References into Functions

Here the authors show that the AF-1 domain of AR is bound by the cochaperone Bag-1L. PMID:28826504
Over expression of BAG-1 may be a biomarker for cisplatin resistance in patients with primary or recurrent head and neck squamous cell carcinoma. PMID:28877725
Increased BAG-1 by PD-L1 confers resistance to tyrosine kinase inhibitor in non-small cell lung cancer via persistent activation of ERK signaling. PMID:28892778
BAG-4/SODD, HSP70, and HSP90 are potential prognostic markers in node-negative breast carcinoma that merit further research. PMID:27038683
interaction with Bag1 then shifts hERG degradation to the membrane-anchored E3 ligase TRC8 and its E2-conjugating enzyme Ube2g2, as determined by siRNA screening PMID:27998983
These results suggest that Bag1 and Bag3 control the stability of the Hsc70-client complex using at least two distinct protein-protein contacts, providing a previously under-appreciated layer of molecular regulation in the human Hsc70 system. PMID:27474739
Taken together, these data indicate that Bag-1 up-regulation is required to maintain apoptosis resistance in human cytomegalovirus-infected cells. PMID:26087710
the combined effects of BAG-1 and XPD polymorphisms on chemotherapy sensitivity and survival in patients with advanced non-small-cell lung cancer, which might be the important predictive markers for platinum-based chemotherapy efficacy. PMID:26124006
Bag-1 indirectly affects cell survival through c-Myc activated signalling that causes elevation of ornithine decarboxylase levels, leading to an increase of the polyamine content. PMID:26178413
Overexpression of miR-138 inhibited cell proliferation by directly suppressing the expression of Bag-1. PMID:25962180
The expression of BAG-1 was upregulated in chronic non-eosinophilic rhinosinusitis. PMID:24830325
BAG-1 expression was high in esophageal carcinoma tissues compared to normal esophagus. After Eca109 cells were transfected with BAG-1-siRNA, the proliferation and invasive capabilities of the cells were decreased while the apoptosis rate was enhanced. PMID:25069471
BAG-1M mediates Hsp70 inhibition downstream of NF-kappaB. PMID:24876225
Forced Bag-1L expression prevented Cisplatin-induced apoptosis. PMID:24969872
distinct isoforms of BAG-1 had different anti-apoptotic ability in breast cancer cells treated with the 4-OH TAM. PMID:24557447
BAG-1M and HspBP1 had differential impacts on the dynamic composition of steroid receptor folding complexes PMID:24454860
GARRPR in Bag-1L as a novel BF-3 regulatory sequence important for fine-tuning the activity of the AR PMID:24523409
The results present that the constructed shRNA plasmids significantly inhibited the expression of mRNA and protein of Lo Vo cell BAG-1, and can maintain the effect for a long term PMID:24061638
Bag1 is expressed at a higher level in functioning- compared to non-functioning pituitary adenomas. PMID:23023342
we measured the binding of human Hsp72 (HSPA1A) to BAG1, BAG2, BAG3, and the unrelated NEF Hsp105. These studies revealed a clear hierarchy of affinities: BAG3 > BAG1 > Hsp105 >> BAG2. PMID:24318877
BAG1 expression provides significant prognostic information when added to the classical clinicopathological parameters and IHC4, particularly in node-positive patients. PMID:23852223
Chlamydia up-regulate Bag-1 via the MAPK/ERK survival pathway. PMID:23708800
BAG-1 suppresses expression of the key regulatory cytokine transforming growth factor beta (TGF-beta1) in colorectal tumour cells. PMID:23108401
Results for the first time indicated that BAG-1 was dysregulated in HCC and suppression of BAG-1 expression which resulted in inhibiting of NF-kappaB signaling might be developed into a new strategy in HCC therapy. PMID:23553841
we identified Caspase 3, BAG1 and BAG3 as key targets of ERalpha in neuronal cells that may play a role in ERalpha-mediated neuroprotection. PMID:23271418
BAG-1 expression in meningioma depends upon WHO pathologic classification and is decreased in higher tumor classification PMID:23322525
The expression of anti-apoptosis gene Bcl-2 associated anthanogen-1 (Bag-1), has been associated with outcome in several cancer types, however its prognostic role in pancreatic cancer is unknown. PMID:22939115
Studied expression of BAG-1 and PARP-1 in LSILs, HSILs and SCCs. Research suggests BAG-1 expression could contribute to the differentiation between LSIL and HSIL/SCC whereas PARP-1 could be useful to the differentiation between HSIL HPV-related and SCC. PMID:22454210
A peptide sequence derived from the cochaperone Bag-1 modulates the unfolded protein response and induces prostate cancer cell death.( PMID:23049684
BAG-1 is highly expressed in the majority of colorectal tumours, targeting the Rb-BAG-1 complex to promote apoptosis. PMID:23059827
ERCC1 and BAG-1 are determinants of survival after surgical treatment of NSCLC, and its mRNA expression in tumor tissues could be used to predict the prognosis of NSCLC treated by platinum. PMID:22439756
Expression of BAG-1 was closely associated with the sensitivity to platinum-based chemotherapeutics in non-small cell lung carcinoma patients. PMID:22179630
Decreased allelic frequency of the BAG-1 rs706118 SNP was observed in patients with frontotemporal lobar degeneration as compared with controls, whereas allelic frequency of the SNP in patients with Alzheimer's disease was similar to controls. PMID:21157029
BAG1 protein was found highly expressed in children with acute myeloid leukemia PMID:22016818
BAG1 induction is essential for protecting cells from MYC-induced apoptosis. PMID:21986497
Bag-1 protein was found to be overexpressed in colorectal cancer. PMID:21809033
silencing of the BAG-1 gene resulted in changes in expression of apoptosis-related genes and sensitized A549 and L9981 cells to cisplatin-induced apoptosis. PMID:20404511
Overexpression of Bag-1 in poorly differentiated SCC may confer both enhanced tumor cell growth and apoptosis resistance. Bag-1 may contribute to the resistance of more advanced epidermal SCC to chemotherapy. PMID:21522149
Overexpression of BAG-1 and p53 plays an important role in the process laryngeal squamous cell carcinoma development. PMID:16398049
These data suggest that IGFBP3 and BAG1 may be key markers of radiosensitivity that enhance the susceptibility of squamous cell esophageal cancer to radiotherapy PMID:21195059
Bag1 induced binding of immature BCR-ABL to proteasome. PMID:20675402
BAG-1 nuclear isomorph appeared more frequently in grade 2 endometrial tumors than in grade 1 and 3 tumors, and the cytoplasmatic isomorph was expressed more strongly than the nuclear one. PMID:21036726
Bag-1 proteins function as regulators of the action of selective transcription factors. PMID:20858459
Expression of Bag-1 in colon cancer was closely correlated with pathologic grade, distance metastasis, Duke stage, and prognosis, but it had no effect on the pathologic type, tumor diameter, depth of invasion, and lymphoid node metastasis of the cancer. PMID:20409521
aberrant expression and subcellular distribution of Bag-1 might play an important role in the malignant transformation of gastric epithelial cells and should be considered as a biomarker for gastric carcinogenesis, subsequent progression, and prognosis. PMID:20096920
Wild-type DJ-1 possesses chaperone activity, which is abolished by the L166P mutation which is reversed by the expression of the cochaperone BAG1. PMID:20156966
BAG-1's effects were associated with an activation of RAF-1-a known binding partner of BAG-1, enhanced signaling through the MAP kinase pathway and a decrease in BIM expression. PMID:19881545
BAG-1, an anti-apoptotic tumour marker Review PMID:12049201
nuclear BAG-1 expression is a useful predictive factor for distant metastasis and a poor prognosis in patients with colorectal cancer. PMID:12402153
BAG-1 may function to suppress the GADD34-mediated cellular stress response and have a role in the survival of cells undergoing stress PMID:12724406

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Subcellular Location

[Isoform 1]: Nucleus. Cytoplasm. Note=Isoform 1 localizes predominantly to the nucleus.; [Isoform 2]: Cytoplasm. Nucleus. Note=Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock.; [Isoform 4]: Cytoplasm. Nucleus. Note=Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.

Tissue Specificity

Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tu

Database Links

HGNC: 937

UNIGENE: Hs.377484

KEGG: hsa:573

STRING: 9606.ENSP00000420514

OMIM: 601497

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