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Acetyl-Histone H2B type 1-B (K20) Recombinant Monoclonal Antibody

Acetyl-Histone H2B type 1-B (K20) Recombinant Monoclonal Antibody

Code: CSB-RA010402A20acHU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, ICC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P33778

Target Names

HIST1H2BB

Alternative Names

HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody

Species Reactivity

Human

Immunogen

A synthesized peptide

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21F11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, ICC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:5000-1:10000
ICC	1:50-1:500
IF	1:30-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30min
All lanes Acetyl-Histone H2B type 1-B(K20)antibody at 0.135μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa
Immunocytochemistry analysis of CSB-RA010402A20acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010402A20acHU at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing Hela cells stained with CSB-RA010402A20acHU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Description

Histone H2B acetylation at lysine 20 represents a key epigenetic modification that influences chromatin accessibility and transcriptional regulation. This post-translational mark on Histone H2B type 1-B plays an important role in modulating gene expression programs, making it a valuable target for researchers investigating chromatin dynamics, epigenetic signaling, and nuclear processes.

This recombinant monoclonal antibody, generated from clone 21F11 in rabbit, offers the reproducibility that epigenetics research demands. Because the antibody sequence is defined and production is cell-line based, you can expect consistent performance across experiments and between lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations.

Validation data demonstrates reliable detection across multiple experimental platforms. In western blot applications, the antibody detects a band at the expected 15 kDa molecular weight in HeLa whole cell lysates treated with sodium butyrate, a histone deacetylase inhibitor that enriches acetylated histones. This confirmation of predicted band size reflects clean, specific target recognition. The antibody has also been validated for immunocytochemistry, immunofluorescence, and flow cytometry using HeLa cells, giving you flexibility to examine acetyl-H2B K20 distribution through complementary visualization approaches. Immunofluorescence staining with DAPI counterstaining confirms the expected nuclear localization pattern, while flow cytometry enables quantitative population-level analysis of this modification.

Supplied in a glycerol-containing buffer for stability, this affinity-purified antibody is well-suited for researchers exploring histone modifications, chromatin remodeling, and epigenetic mechanisms in human samples. Whether you're characterizing acetylation dynamics in response to HDAC inhibitors or mapping modification patterns across different cellular states, this antibody provides a dependable tool for your epigenetics workflow.

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Target Background

Function(From Uniprot)

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene References into Functions

Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants has been uncovered. PMID:29643390
RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response. PMID:26854224
RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling PMID:21362548

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Subcellular Location

Nucleus. Chromosome.

Protein Families

Histone H2B family

Database Links

HGNC: 4751

UNIGENE: Hs.553494

KEGG: hsa:3018

STRING: 9606.ENSP00000350580

OMIM: 602803

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