Alternative Names
Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ
Immunogen
A synthesized peptide derived from Histone H3 (acetyl K18)
Immunogen Species
Homo sapiens (Human)
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Tested Applications
ELISA, IHC, IF, FC
Recommended Dilution
| Application | Recommended Dilution |
|---|
| IHC | 1:50-1:200 |
| IF | 1:50-1:200 |
| FC | 1:50-1:200 |
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Images
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IHC image of CSB-RA547773A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.
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IHC image of CSB-RA547773A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.
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Immunofluorescence staining of A549 with CSB-RA547773A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 490-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Overlay Peak curve showing A549 cells stained with CSB-RA547773A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Description
Acetylation of histone H3 at lysine 18 represents a critical epigenetic modification that plays a central role in transcriptional regulation and chromatin remodeling. This modification mark is associated with active gene expression and has emerged as an important focus in cancer research, where aberrant histone acetylation patterns contribute to oncogenic processes. Understanding the dynamics of H3K18 acetylation provides valuable insights into gene regulatory mechanisms and disease pathogenesis.
This recombinant monoclonal antibody, clone 30A7, offers researchers the consistency and reliability essential for epigenetic studies. Produced using recombinant technology with a sequence-defined rabbit IgG framework, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and reproducibility. The defined sequence ensures that experiments conducted months or years apart will yield comparable results, a particularly important consideration when building datasets over extended research programs.
Validation across multiple applications demonstrates this antibody's versatility in your experimental workflows. Immunohistochemistry studies have confirmed robust nuclear staining in paraffin-embedded human breast cancer and gastric cancer tissues at 1:100 dilution, making it well-suited for investigating H3K18 acetylation patterns in tumor samples. Immunofluorescence analysis in A549 human lung carcinoma cells reveals clear nuclear localization consistent with the expected chromatin-associated distribution of this histone modification. Flow cytometry validation in A549 cells further confirms the antibody's utility for quantitative single-cell analysis of acetylation status.
Whether you are investigating epigenetic dysregulation in cancer, studying chromatin dynamics, or exploring nuclear signaling pathways, this antibody provides a reliable tool for detecting acetyl-H3K18 across tissue-based and cell-based applications.
