AKT1/2/3 Recombinant Monoclonal Antibody

Code: CSB-RA272392A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, IHC, FC

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Target Background

Product Details

Uniprot NO.

P31749 P31751 Q9Y243

Target Names

AKT1/2/3

Alternative Names

RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha), AKT1, PKB RAC, RAC-beta serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-2) (Protein kinase B beta) (PKB beta) (RAC-PK-beta), AKT2, RAC-gamma serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-3) (Protein kinase B gamma) (PKB gamma) (RAC-PK-gamma) (STK-2), AKT3, PKBG

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human AKT1/2/3 Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16E10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate
All lanes: CD44 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
IHC image of CSB-RA272392A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.
Overlay Peak curve showing Jurkat cells stained with CSB-RA272392A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

The AKT kinase family, comprising AKT1, AKT2, and AKT3, represents a central node in the PI3K/AKT signaling pathway that governs fundamental cellular processes including survival, proliferation, metabolism, and growth. Dysregulation of AKT signaling is implicated in numerous cancers and metabolic disorders, making these kinases essential targets for researchers investigating oncogenic mechanisms, insulin signaling, and therapeutic intervention strategies.

This recombinant monoclonal antibody (clone 16E10) detects all three AKT isoforms, providing comprehensive coverage of this kinase family in a single reagent. Developed using recombinant technology with a defined immunogen sequence, this antibody offers the lot-to-lot consistency critical for longitudinal studies and reproducible quantification of AKT expression across experimental conditions. The rabbit IgG format ensures high affinity binding while the affinity-chromatography purification delivers excellent signal-to-noise performance.

Validation studies demonstrate robust performance across multiple experimental platforms. Western blot analysis in SH-SY5Y neuroblastoma cells confirms detection at the expected 56 kDa molecular weight, with recommended dilutions of 1:500–1:2000 providing flexibility for optimization. Immunohistochemistry validation in human brain tissue sections using citrate buffer antigen retrieval shows clear staining patterns suitable for spatial expression analysis. Flow cytometry characterization in Jurkat T cells demonstrates effective intracellular detection following formaldehyde fixation and Triton X-100 permeabilization, with clear separation from isotype control at 1:50–1:200 dilutions.

This antibody serves researchers investigating cancer biology, metabolic signaling, epigenetic regulation, and signal transduction pathways where AKT activity plays a determining role in cellular outcomes.