ACTA2 Recombinant Monoclonal Antibody

Code: CSB-RA163563A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse

Application: ELISA, WB, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P62736

Target Names

ACTA2

Alternative Names

Actin, aortic smooth muscle (Alpha-actin-2) (Cell growth-inhibiting gene 46 protein) [Cleaved into: Actin, aortic smooth muscle, intermediate form], ACTA2, ACTSA ACTVS

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human ACTA2 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6E11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), HT-29 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), A431 whole cell lysate(30µg), A375 whole cell lysate(30µg), Mouse duodenum tissue lysate(30µg)
All lanes:alpha Smooth muscle actin antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time：20s
IHC image of CSB-RA163563A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA163563A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cell with CSB-RA163563A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing HepG2 cells stained with CSB-RA163563A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Alpha-smooth muscle actin (ACTA2) serves as a definitive marker for smooth muscle cells and myofibroblasts, making it indispensable for research into vascular biology, fibrosis, wound healing, and epithelial-to-mesenchymal transition. This cytoskeletal protein plays critical roles in contractile function and is frequently upregulated in pathological conditions including organ fibrosis and tumor stroma remodeling, positioning it as a key target for both basic research and translational studies.

This recombinant rabbit monoclonal antibody (clone 6E11) offers the reproducibility that demanding experimental workflows require. Because recombinant production ensures a sequence-defined, consistent reagent across lots, researchers can confidently compare results across extended studies without the variability often encountered with traditional hybridoma-derived antibodies.

Validation data demonstrates robust performance across multiple platforms. Western blot analysis detects ACTA2 in diverse human cell lines including HeLa, HT-29, COLO-205, HEK293, A431, and A375, as well as mouse duodenum tissue, confirming cross-species reactivity with human and mouse samples. The observed band at approximately 45 kDa runs slightly higher than the predicted 42 kDa molecular weight, likely attributes to the mobility deviation of ACTA2 in SDS-PAGE (influence of amino acid composition/conformation) or minor post-translational modifications (such as low-abundance phosphorylation/glycosylation). Immunohistochemistry staining has been validated in human glioma and kidney tissue sections, while immunofluorescence and flow cytometry applications have been confirmed using HepG2 cells, demonstrating clear cytoplasmic localization patterns.

This versatility across western blotting, immunohistochemistry, immunofluorescence, and flow cytometry provides flexibility for researchers investigating smooth muscle differentiation, cancer-associated fibroblasts, or fibrotic disease mechanisms. The antibody arrives purified by affinity chromatography in a glycerol-containing buffer optimized for long-term storage stability.

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Target Background

Function(From Uniprot)

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Gene References into Functions

Adenocarcinomas showed significantly higher staining scores of both VEGF and alphaSMA than squamous cell carcinomas did. In 42 cases of high CD31 score, five-year survival rate (87%) of patients with lung cancer showing mature tumor vessels was significantly better than that (69%) of patients with immature tumor vessels PMID:29970531
connective tissue disease including ACTA2 mutations should be considered for aortic dissection in young adult patients. PMID:28808903
cellular defects due to the ACTA2 mutation in both aortic smooth muscle cells and adventitial fibroblasts may contribute to development of thoracic aortic aneurysms and dissections and proliferative occlusive vascular disease PMID:28652363
our results seemed to justify the conclusion that ACTA2 did not play a significant role in the pathogenesis of BAV aortopathy. PMID:29049801
In patients with MYH11 or ACTA2 variants, the effect of intronic variants on splicing was demonstrated on the mRNA level in the induced smooth muscle cell (SMC), allowing classification into pathogenic or nonpathogenic variants. PMID:28074631
We present a young woman whose ACTA2 mutation was ascertained during pregnancy because of her father's history of dissecting aneurysms. She was delivered at full term by cesarean section and subsequently had severe uterine hemorrhage due to uterine atony. Targeted analysis of the patient's ACTA2 gene revealed she had inherited the N117S variant from her father. PMID:29202781
During transition from the pluripotent stage towards the neural developmental stage, ACTA2 is differentially expressed in bipolar patient derived cells compared to control derived cells. PMID:28117838
Two unrelated patients with the heterozygous R189H mutation in ACTA2 and complex congenital heart defects expands the cardiac phenotype of multisystemic smooth muscle dysfunction syndrome. PMID:28328125
Cells in high glucose for 7 days showed a significant decrease in mRNA expression of CD31 and VE-cadherin, and a significant increase in that of alpha-SMA and collagen I. PMID:28347704
ACTA2 is the isoform of contractile protein alpha-actin present in vascular smooth muscle cells (SMCs) throughout the arterial tree.4 Pathologic conditions in individuals with ACTA2 mutations show increased deposition of SMCs in the intimal arterial layer, leading to a decreased intraluminal diameter.4 PMID:28343608
Two novel actin alpha 2 mutations (N117I and L348R) were identified in each familial non-syndromatic thoracic aortic aneurysm proband separately, and an additional novel actin alpha 2 mutation (Y168N) was identified in one patient with sporadic non-syndromatic thoracic aortic aneurysm. PMID:27431987
The R179H mutation has the potential to affect actin structure and function in both the contractile domain of the cell and the more dynamic cytoskeletal pool of actin, both of which are required for contraction. PMID:27551047
site-directed mutagenesis revealed several basic amino acid residues in the intermolecular (R267) and intramolecular (K82 and R159) subdomains that are essential for Purbeta transcriptional repressor function in Acta2 promoter-reporter assays. In keeping with their diminished Acta2 repressor activity in fibroblasts, purified Purbeta variants containing an R267A mutation exhibited reduced binding affinity for purine-ric... PMID:27064749
Data show that the expression profiles of three proteins: E-cadherin, Snail, and alpha-smooth muscle actin were significantly different in extraprostatic extension prostate cancer (PCa) compared with intra-prostatic tumour. PMID:26701730
ACTA2 mutations are associated with structural disruption and functional impairment of contractile proteins, and predispose to a variety of diffuse vascular diseases including TAAD, CAD, ischemic strokes, and Moyamoya disease. Vascular SMCs are also implicated in vascular remodeling in both physiological and pathological conditions. PMID:26934405
Parenchymal alphaSMA expression in hepatic tissue appeared to increase only among hepatitis C subjects with fibrosis 3-4. PMID:26927700
Multisystemic smooth muscle dysfunction syndrome secondary to an ACTA2 mutation. PMID:26835993
alpha-SMA expression, and MLC phosphorylation, whereas butaprost inhibited TGF-beta2-induced CPCG contraction, actin polymerization, and MLC phosphorylation. PMID:27082296
Genetic analysis revealed a missense mutation of the ACTA2 gene, encoding for a smooth muscle isoform of alpha-actin. PMID:27012699
NGF has a role in modulating trkANGFR/p75NTR in alphaSMA-expressing conjunctival fibroblasts from human ocular cicatricial pemphigoid PMID:26569118
increased level of alphaSMA, a hallmark of epithelial mesenchymal transition in lens epithelial cells, is associated with increased level of histone H4 acetylation at the promoter region of alphaSMA gene(ACTA2) PMID:25853442
Mutations disrupting p.R179 and p.R258 were associated with significantly increased risk for aortic events, whereas p.R185Q and p.R118Q mutations showed significantly lower risk of aortic events compared with other mutations PMID:25759435
Alpha-SMA overexpression induced YAP translocation to the nucleus and reduced the high clonogenicity and adipogenic potential of alpha-SMA-negative MSCs. PMID:26028530
The number and distribution of myofibroblasts and the expression levels of ACTA2 in the fetal membrane may be involved in the mechanisms of development, apoptosis and trophoblastmyofibroblast transformation of the fetal membrane. PMID:25954927
All the variants analyzed expressed alpha-SMA mildly or moderately, except for the follicular variant that either did not express alpha-SMA or expressed it mildly. PMID:26470744
alphaSMA was not expressed in benign papillary hyperplastic lesions while it was expressed in papillary carcinoma PMID:25921136
Transforming growth factor-beta1 induces an up-regulation of alpha-SMA stress fibers in retinal Muller cells and fibroblasts and appears to have a cell-specific effect on intracellular collagen expression. PMID:26447986
Ca2+- and KCa3.1-dependent processes facilitate "constitutive" alpha smooth muscle actin expression and Smad2/3 signalling in IPF-derived fibroblasts, and thus promote fibroblast to myofibroblast differentiation. PMID:25476248
The effect of the R258C mutation in SM alpha-actin, is reported. PMID:26153420
ACTA2, FSP1, and PDGFRa are unfavorable prognostic indicators of esophageal squamous cell carcinoma patients. PMID:24945657
The immunohistochemical expression of E-cadherin and alpha-smooth muscle actin (alpha-SMA) in 15 cases of pleomorphic adenoma of salivary glands, were investigated. PMID:25611270
Epithelial-mesenchymal transition-related proteins CK-7 and alpha-SMA colocalized to the intrahepatic biliary epithelial cells in patients with biliary atresia. PMID:25406900
The ACTA2 gene encodes the smooth muscle alpha2-actin protein. Pathogenic mutations within ACTA2 result in disrupted contractility and are a recognized cause of aortic dissection PMID:26034244
We report a missense mutation in the smooth muscle alpha-actin (ACTA2; MIM*102620) gene in a 3 generational family from Northern Ireland in which iris flocculi were an ocular marker of the disease. PMID:24020716
We report a case of ACTA2 mutation in a 3-year-old girl presenting with acute ischemic stroke PMID:24353327
Women with ACTA2 mutations who are planning to get pregnant should be counseled about this risk of aortic dissection, and proper clinical management should be initiated to reduce this risk. PMID:24243736
alpha-SMA could be a useful marker for the detection of early stage fibrosis in liver transplant recipients stopping immunosuppressive therapy. PMID:24966580
This study analyzes the microvascular density (MVD) for CD105+ and alpha-SMA+ vessels and VEGF immunoexpression for 35 oral squamous cell carcinomas and for the associated dysplastic lesions of the lips. PMID:24715163
This case illustrates the spectrum of systemic malformations that are attributable to mutations in ACTA2 and expands the spectrum of cerebrovascular anomalies that are now known to accompany congenital mydriasis. PMID:24998021
Identical twin brothers were identified with severe progressive thoracic aortic aneurysm due to ACTA2 mutation. PMID:25225139
Relationships of alpha-SMA-positive fibroblasts and SDF-1-positive tumor cells with neoangiogenesis in nasopharyngeal carcinoma. PMID:24877105
High ACTA2 expression is associated with pancreatic cancer. PMID:25314063
genetic testing revealed new mutations in FBN1, TGFbetaR1, TGFbetaR2 and ACTA2 detected in patients with clinical diagnosis of Marfan Syndrome, Loeys-Dietz Syndrome and Thoracic Aortic Aneurysms and Dissections. PMID:24793577
Significant down-regulation of the ACTA2 gene, encoding the cytoskeletal protein alpha 2 actin, in response to RHOA knockdown in both osteoblast-like and osteoclast-like cells. PMID:24840563
Sphingosylphosphorylcholine stimulates alpha-SMA protein expression and human lung fibroblast mediated collagen gel contraction via S1P2 receptor. PMID:24614064
Suggest dynamic interplay between transcriptional activators Pur-alpha/Pur-beta and repressors in regulating SMalphaA gene output during myofibroblast differentiation. PMID:24446247
Angiogenesis evaluated through the VEGF and MVD (CD105+ and alpha-SMA+) expression is correlated with the progression and metastasis of gastric cancer and could be considered a prognostic marker of these tumors. PMID:24322015
ACTA2 regulates c-MET and FAK expression in lung adenocarcinoma cells, which positively and selectively influence metastatic potential. PMID:23995859
findings confirmed that ACTA2 did not play an important role in the pathogenesis of moyamoya disease PMID:24024919
Our data suggest that ACTA2 is not a major disease-causing gene for spontaneous cerebral artery dissection PMID:23879759