ABCG1 Recombinant Monoclonal Antibody

Code: CSB-RA146479A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC

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Target Background

Product Details

Uniprot NO.

P45844

Target Names

ABCG1

Alternative Names

ABC transporter 8 antibody; ABC8 antibody; ABCG1 antibody; ABCG1_HUMAN antibody; ATP-binding cassette sub family G member 1 antibody; ATP-binding cassette sub-family G member 1 antibody; ATP-binding cassette transporter 8 antibody; ATP-binding cassette transporter member 1 of subfamily G antibody; ATP-binding cassette; sub family G WHITE member 1 antibody; homolog of Drosophila white antibody; MGC34313 antibody; White protein homolog antibody; White protein homolog ATP binding cassette transporter 8 antibody; WHITE1 antibody; WHT1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human ABCG1 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: hela whole cell lysate(30µg), A549 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), A431 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg)
All lanes:ABCG1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 76 kDa
Observed band size: 110 kDa
Exposure time：2min
IHC image of CSB-RA146479A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

ABCG1 is a member of the ATP-binding cassette transporter superfamily that plays a central role in cellular cholesterol and phospholipid homeostasis. This transporter facilitates the efflux of cholesterol to high-density lipoprotein particles, making it essential for reverse cholesterol transport and the prevention of lipid accumulation in peripheral tissues. In neuroscience research, ABCG1 has garnered significant attention for its involvement in brain lipid metabolism, where dysregulation has been implicated in neurodegenerative conditions and cognitive decline.

This recombinant monoclonal antibody, generated against a synthesized peptide from human ABCG1, offers the reproducibility and consistency that demanding research protocols require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit IgG format provides excellent signal-to-noise characteristics across multiple detection platforms.

Validation studies demonstrate reliable performance in western blotting across a diverse panel of human cell lines, including HeLa, A549, Jurkat, A431, PC-3, and SH-SY5Y neuroblastoma cells. The observed band at approximately 110 kDa, rather than the predicted 76 kDa molecular weight, reflects the extensive post-translational modifications characteristic of ABC transporters, particularly glycosylation events that are critical for proper membrane localization and function. Immunohistochemistry validation in paraffin-embedded human kidney tissue confirms utility for tissue-based studies, with recommended dilutions ranging from 1:50 to 1:200.

Whether investigating cholesterol efflux mechanisms, exploring lipid-related pathways in neurological disease models, or characterizing ABCG1 expression patterns across tissue types, this antibody provides the flexibility and reliability needed for rigorous experimental workflows.

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Target Background

Function(From Uniprot)

Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol.

Gene References into Functions

HSP70 suppresses the expression of ABCA1 and ABCG1 through preventing Elk-1 from binding to the promoter of ABCA1 and ABCG1 in human THP-1-derived macrophages. PMID:29678642
Downregulation of ABCG1 in macrophages promoted atherosclerotic lesions. PMID:30393020
High ABCG1 expression is associated with glioma. PMID:26981778
ABCG1 regulates pulmonary surfactant metabolism PMID:28264879
Hepatic free cholesterol content was significantly increased in NASH as compared to non-NASH subjects, while ABCA1 and ABCG1 protein levels significantly decreased with NASH and fibrosis progression. The relative expression of miR-33a and miR-144 correlated inversely with ABCA1 but not with ABCG1 protein levels. miR-33a/144 and their target gene ABCA1 may contribute to the pathogenesis of NASH in morbidly obese subjects. PMID:26945479
Understanding the relationship between cholesterol and inflammation in the lung, and the role that ABC transporters play in this may illuminate new pathways to target for the treatment of inflammatory lung diseases PMID:28241820
Findings suggest that the ABCG1-mediated efflux of cholesterol, but not of 7-ketocholesterol, shows specificity for structural domains of apoA-I bound to reconstituted HDL. Although the mid region alone of apoA-I associated to rHDL can promote ABCG1-mediated cholesterol efflux, deletion of carboxyl-terminal region 185-243 from full-length apoA-I diminishes ABCG1-mediated cholesterol efflux. PMID:23826352
ABCG1 regulates T cell differentiation into Tregs, highlighting a pathway by which cholesterol accumulation can influence T cell homeostasis in atherosclerosis PMID:27482882
ABCG1 and ABCG4 alter the distribution of gamma-secretase on the plasma membrane, leading to the decreased gamma-secretase activity and suppressed Abeta secretion PMID:27196068
Both the full-length and the short isoforms of ABCG1 can dimerize with ABCG4, whereas the ABCG2 multidrug transporter is unable to form a heterodimer with ABCG4. PMID:27228027
DNA methylation at the ABCG1 locus cg06500161 in blood DNA was associated with an increased risk for future type2 diabetes. PMID:27148772
we have newly identified a haplotype-tagging SNP, rs225396, in ABCG1 to be associated with PCV and nAMD in Chinese and Japanese cohorts. This provides new evidence to support ABCG1 as a susceptibility gene for PCV and nAMD. PMID:27787563
ABCG1 gene expression positively correlated with obesity indicators. PMID:27420620
Our results indicated that genetic variants of ABCG1 may be predictors of survival of nonsmall-cell lung cancer patients PMID:26757251
This review focuses on the role of ABC transporters A1 and G1 in the pathogenesis of atherosclerosis PMID:27239842
Leu at position 550/562 in mABCG1/hABCG1 is critical for their plasma membrane localization but not for ABCG1-mediated cholesterol efflux. PMID:26695502
ABCG1 polymorphisms are associated with reduced risk of developing ischemic stroke in hypertriglyceridemic population and atherothrombotic stroke in this cohort of Chinese Han population. PMID:25890853
Data suggest that ligand-induced activation of RAGE (advanced glycosylation end product-specific receptor) down-regulates ABCG1-mediated cholesterol efflux from macrophages leading to diabetic angiopathies and atherosclerotic plaques. PMID:26253613
Propofol up-regulates expression of ABCA1, ABCG1, and SR-B1 through the PPARgamma/LXRalpha pathway in THP-1 macrophage-derived foam cells. PMID:25600616
Expression analysis revealed an association between ABCG1 methylation and lipid levels that might be partly mediated by ABCG1 expression. PMID:25583993
HUWE1 and NEDD4-1 are two E3 ligases that are fundamental enzymes in the post-translational regulation of ABCG1 and ABCG4 protein levels and cellular cholesterol export activity PMID:26296893
These findings indicate that SNP rs1893590 of ABCG1 has a significant impact over HDL-C under asymptomatic clinical conditions in an age and BMI dependent way. PMID:25398214
ABCG1 promoter rs57137919G>A variant had an allele-specific effect on ABCG1 expression and was associated with an increased apoptosis in cholesterol-loaded macrophages. PMID:24972087
The present review summarizes the current knowledge and views on the regulatory role of CAV1 on the cholesterol homeostasis with emphasis on the association of CAV1 with ABCA1 and ABCG1. [review] PMID:24801727
Hyperalphalipoproteinemics present a decrease in hsa-miR-33a and higher mRNA expression of ABCA1 and ABCG1. PMID:26051418
ABCG1 can interact with cholesterol via a functional CRAC domain (cholesterol recognition/interaction amino acid consensus motifs). PMID:25732853
Ang-(1-7) upregulates ABCA1 and ABCG1 expression. PMID:25225013
These results suggest that ABCA1, ABCG1, and ABCG4 are localized to distinct membrane meso-domains and disturb the meso-domain structures by reorganizing lipids on the plasma membrane PMID:25302608
The role of cellular cholesterol transport proteins including adenosine triphosphate binding cassette transporter A1 (ABCA1), G1 (ABCG1) and scavenger receptor class B type I (SR-BI) in diabetic nephropathy, was determined. PMID:25181357
promoter DNA hypermethylation of the ABCG1 and GALNT2 genes, but not the HMGCR gene, is associated with an increased risk of CHD. PMID:25084356
The study identifies a major role of adipocyte ABCG1 in adiposity and fat mass growth and suggests that adipose ABCG1 might represent a potential therapeutic target in obesity. PMID:25249572
knock-outs display expansion of B-1 B cells, which secrete natural Abs that protect from atherosclerosis PMID:25339664
Decrease in the ABCG1 gene expression in macrophages is associated with atherosclerosis. PMID:25509420
I human macrophages, polyunsaturated fatty acids such as EPA have an effect on the cholesterol homeostasis in macrophages, and they can change the expression of ABCG1 gene. PMID:24901717
Generation of extracellular cholesterol microdomains is mediated by ABCG1. PMID:24212237
These findings indicate that CAV1 interacts with ABCG1 and regulates ABCG1-mediated cholesterol efflux. PMID:24576892
Data suggest that in placentas from women with pre-eclampsia, expression of ABCA1 (ABC transporter 1) is down-regulated in apical membrane of villous syncytiotrophoblast and in villous fetal endothelial cells; expression of ABCG1 is unchanged. PMID:23880356
results suggest that reduction of ABCG1 induces endothelial apoptosis, which seems associated with intracellular free cholesterol accumulation and subsequent ER stress PMID:23897420
Our results showed that CETP is a susceptibility gene for neovascular age-related macular degeneration and polypoidal choroidal vasculopathy (PCV) and that ABCG1 a putative gene for PCV. PMID:24393350
No significant associations were detected for the ABCB6 or ABCG1 gene PMID:24192121
ABCA1 and ABCG1 ubiquitination and degradation are regulated by cellular cholesterol PMID:24500716
MiR-128-2 inhibits the expression of ABCA1, ABCG1 and RXRalpha directly through a miR-128-2-binding site within their respective 3'untranslated regions. PMID:23990020
Two highly conserved residues, Asn and Phe, play an important role in ABCG1-dependent export of cellular cholesterol through the regulation of ABCG1 trafficking. PMID:24320932
LXRalpha plays a central role in neopterin-induced downregulation of ABCA1 and ABCG1 in THP-1 macrophage-derived foam cells. PMID:23564066
Our data suggest that MCP-1 impairs RCT activity in HepG2 cells by a PI3K/Akt-mediated posttranslational regulation of ABCA1, ABCG1, and SR-BI cell-surface expression. PMID:23402987
Evidence is presented for the first time suggesting that resveratrol is able to activate ABCG1 activity by an alternative mechanism that involves an indirect interaction PMID:23770099
Endothelial dysfunction induced by high glucose is associated with decreased ABCG1 expression. PMID:22365996
ABCG1 is an active lipid transporter and possesses different binding sites for cholesterol and sphingomyelin PMID:23172659
Genetic variations in ABCA1 and ABCG1 were not associated with increased risk of type 2 diabetes in the general population. PMID:23139370
These data indicate that ABCA1, ABCG1, and SR-BI are reduced in various populations under subclinically inflammatory conditions, which may potentially lead to impairing reverse cholesterol transport and developing atherosclerosis. PMID:22614118

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell membrane. Note=Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.

Protein Families

ABC transporter superfamily, ABCG family, Eye pigment precursor importer (TC 3.A.1.204) subfamily

Tissue Specificity

Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.

Database Links

HGNC: 73

UNIGENE: Hs.124649

KEGG: hsa:9619

STRING: 9606.ENSP00000354995

OMIM: 603076

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