Recombinant Mouse Talin-2 (Tln2), partial

Code: CSB-EP741137MO

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Size:

20ug100ug1mg

US$256

Quantity:

Express system: E.coli

Species: Mus musculus (Mouse)

Tag Info: C-terminal 6xHis-tagged

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Target Background

Product Details

Abbreviation

Recombinant Mouse Tln2 protein, partial

Purity

≥ 85% as determined by SDS-PAGE.

Activity

Not Test

Target Names

Tln2

Uniprot NO.

Q71LX4

Research Area

Cell Biology

Alternative Names

Tln2

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-406aa

Mol. Weight

54.0 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

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Target Background

Function(From Uniprot)

As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity.

Gene References into Functions

Loss of all Tln forms from the heart-muscle cell leads to myocyte instability and a dilated cardiomyopathy. PMID:28698364
Binding of vinculin to the R1-R3 region of the talin rod is important for focal adhesion stability. PMID:26634421
Data indicate that talin mechanics are isoform specific so that expression of either talin-1 or talin-2 modulates extracellular rigidity sensing. PMID:26523364
Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload. PMID:23266827
Tln2(cd/cd) mice showed no major difference in body mass or the weight of the major organs compared to wild-type, although they displayed a mildly dystrophic phenotype. PMID:22925892
Talin1 was concentrated in peripheral focal adhesions while talin2 was observed in both focal and fibrillar adhesions, and knock-down of talin2 compromised fibronectin fibrillogenesis PMID:22306379
Fxr1 knockout hearts exhibit an up-regulation of desmoplakin and talin2 proteins, which is accompanied by severe disruption of desmosome as well as costamere architecture and composition in the heart PMID:21659647
Talin2 may serve as the link between integrins and the sarcomeric cytoskeletonin stable adhesion complexes in mature striated muscle. PMID:17183545
Depletion of talin2 in talin1-null cells did not affect the initiation of matrix-activated spreading or Src family kinase activation, but abolished the extracellular matrix-integrin-cytoskeleton linkage, sustained cell spreading and adhesion. PMID:19160486
demonstrate that testis and kidney express truncated talin 2 isoforms that lack the N-terminal half of the protein, and provide evidence for the developmentally regulated expression of the short testis-specific talin 2 isoform in elongating spermatids PMID:19220457
Talin 1 and 2 are crucial for skeletal muscle development, where they regulate myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions. PMID:19793892
Data show that clustered alpha(5)beta(1) integrins, while less stable links to alpha(v)beta(3) integrins initiate mechanotransduction, resulting in reinforcement of integrin-cytoskeleton linkages through talin-dependent bonds. PMID:19805288

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Subcellular Location

Cytoplasm. Cell junction, focal adhesion. Cell junction, synapse. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton.

Database Links

UNIGENE: Mm.33645

KEGG: mmu:70549

STRING: 10090.ENSMUSP00000035272

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