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Recombinant Human Cis-aconitate decarboxylase(ACOD1)

Recombinant Human Cis-aconitate decarboxylase(ACOD1)

Code: CSB-EP011825HUc7

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Size:

20ug100ug1mg

US$388

Quantity:

Express system: E.coli

Species: Homo sapiens (Human)

Tag Info: C-terminal 6xHis-tagged

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Product Details
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Target Background

Product Details

Abbreviation

Recombinant Human ACOD1 protein

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ACOD1

Uniprot NO.

A6NK06

Research Area

Others

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-481aa

Mol. Weight

59.5 kDa

Protein Length

Full Length

Tag Info

C-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

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Target Background

Function(From Uniprot)

Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages. Involved in antiviral response following infection by flavivirus in neurons: ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes. Plays a role in the embryo implantation.

Gene References into Functions

In conclusion, we found that IRG1 promoted MHC I level in macrophages membrane via ROS-STAT1/3-TAP1 pathway. Besides, IRG1 could activate the pentose phosphate pathway to promote production of ROS depending on NADPH oxidases. PMID:28477473
findings suggest that polymorphisms in the immunoresponsive gene 1 (IRG1) gene are associated with the immune response to hepatitis B vaccination; observed that IRG1 inhibited the HBV life cycle and that IRG1 rs17385627 allele 'A' was more effective than rs17385627 allele 'T' at eliminating HBV in HepG2.2.15 cell PMID:28004399
Respiratory syncytial virus infection induced IRG1 expression in human A549 cells and in the lung tissues of respiratory syncytial virus -infected mice and respiratory syncytial virus infection or IRG1 overexpression promoted reactive oxygen species production. PMID:27252532
IRF1 is a transcriptional regulator of IRG1 in human macrophages. PMID:26872335
We identified IRF1 as a transcriptional regulator of IRG1 expression in both human and mouse macrophages under inflammatory conditions. PMID:26872335
IRG1/CAD associates with mitochondria in human macrophages under inflammatory conditions such as LPS and/or IFNg. PMID:26872335
Taken together, the results show that IRG1 is expressed by human monocytes, macrophages and dendritic cells and that it is rapidly induced in both human and murine macrophages under inflammatory conditions such as LPS, TNFa and IFNg. PMID:26872335
Findings revealed that IRG1 is a candidate oncogene that is amplified in glioma and is involved in novel mechanisms that influence glioma pathogenesis. PMID:25216059
inducible IRG1 promotes endotoxin tolerance by increasing A20 expression through ROS, indicating a new molecular mechanism regulating hypoinflammation of sepsis and endotoxin tolerance. PMID:23609450
IRG1 links cellular metabolism with immune defense by catalyzing itaconic acid production PMID:23610393
Irg1 gene encodes an enzyme synthesizing itaconic acid from the TCA cycle intermediate cisaconitate. Furthermore, our data also show that IRG1 mediated itaconic acid production contributes to the antimicrobial activity of macrophages. PMID:23610393
Results indicated that the IRG1 gene is differentially expressed in human fetal PBMCs and LPS-stimulated adult PBMCs. PMID:21424586