Recombinant Human HLA-DMA protein (HLA-DMA), partial

The region for expressing recombinant Human HLA-DMA contains amino acids 27-233. This HLA-DMA protein is theoretically predicted to have a molecular weight of 27.4 kDa. This HLA-DMA recombinant protein is manufactured in e.coli. The N-terminal 6xHis tag was smoothly integrated into the coding gene of HLA-DMA, which enables a simple process of detecting and purifying the HLA-DMA recombinant protein in the following steps.

The human leukocyte antigen-DMA (HLA-DMA) protein is a non-classical MHC class II protein that is involved in the peptide-loading process onto MHC class II molecules. Specifically, HLA-DMA acts as a chaperone during the peptide-loading phase in the endosomal compartments of antigen-presenting cells (APCs). It associates with MHC class II molecules and catalyzes the exchange of peptides bound to the MHC class II binding groove. This peptide exchange mechanism allows for the loading of high-affinity peptides onto MHC class II molecules, optimizing the antigen presentation to CD4⁺ T cells. The HLA-DMA protein is crucial for the efficient presentation of antigens to CD4⁺ T cells, which, in turn, triggers appropriate immune responses against pathogens. Understanding the functions of HLA-DMA is essential for unraveling the complexities of antigen presentation and immune regulation.