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Recombinant Xenopus laevis Sonic hedgehog protein (shh)

Recombinant Xenopus laevis Sonic hedgehog protein (shh)

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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

shh

Uniprot NO.

Q92000

Species

Xenopus laevis (African clawed frog)

Source

Yeast

Expression Region

25-444

Target Protein Sequence

CGPGRG IGKRRHPKKL TPLAYKQFIP NVAEKTLGAS GRYEGKITRN SDCFKELTPN YNPDIMFKDE ESTGADRLMT QRCKDKLNAL AISVMNQWPG VKLRVTEGWD EDGHHLEESL HYEGRAVDIT TSDRDRSKYG MLGRLAVEAG FDWVYYESKA HIHCSVKAEN SVAAKSGGCF PAGARVMVEF GGTKAVKDLR PGDRVLSSDP QGNLLYSDFL MFIDQERDVK KLFYVIETSQ RKIRLTAAHL LFVAQTKVNG TRSFKSVFAS NIQPGDLIYT ADPKTMTLKA VKVEKVDLEE DTGAYAPLTA HGTVVIDQVL ASCYAVIEEH TWAHLAFAPL RFGMSLSSYI YPRDSSPPSG LQPHHQVDLQ SHHQVDLQSH HQVDLQSHHQ LEGIHWYSQL LYQIGTWLLD SNSLHPLGMA TKSS

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Description

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Target Background

Function

The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum.; The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO.

Gene References into Functions

Hh signaling is necessary and sufficient to initiate mouth formation, and Hh activation is required in a dose-dependent fashion to determine the size of the mouth PMID:25300580
Data indicate that sonic hedgehog is expressed exclusively in the notochord but not in the spinal cord of the regenerate. PMID:24941877
Dzip1-dependent stabilization of Spop/HIB is evolutionarily conserved and essential for proper regulation of Gli/Ci proteins in the Hh pathway. PMID:24072710
These results indicate that electrical activity and second-messenger signaling mediate Shh action in embryonic spinal neurons. PMID:21368195
Connective tissue-specific expression of BMP-4 mRNA is up-regulated by sonic hedgehog. PMID:17016847
In an examination of signaling pathways in developing Xenopus lung, shh but not ihh was expressed in the very anterior part of early lung epithelium. PMID:20682360
Data propose that Shh serves as a ventral optic tract repellent that helps to define the caudal boundary for retinal axons in the diencephalon, and that this signaling is also required for initial target recognition at the optic tectum. PMID:20931659
The forebrain of Xenopus revealed a largely conserved pattern of Shh expression among tetrapods. PMID:20540934
Hedgehog regulates superficial slow muscle fibres in Xenopus and tetrapod trunk myogenesis PMID:15201218
Results suggest that 7-dehydrocholesterol reductase and Sonic Hedgehog are co-expressed during midline development in Xenopus embryos PMID:16687448
activated Hedgehog signaling in the blastema and found that target genes of Shh were inducible in the mesenchyme of limb blastema PMID:19544583

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Subcellular Location

Endoplasmic reticulum membrane. Golgi apparatus membrane.; [Sonic hedgehog protein N-product]: Cell membrane; Lipid-anchor.

Protein Families

Hedgehog family

Tissue Specificity

Strongly expressed in notochord and neural floor plate during embryogenesis. In tadpole, high expression is observed in pancreas/stomach, moderate expression in tail, and low expression in intestine, brain, and hind limb.

Database Links

UNIGENE: Xl.866

KEGG: xla:398047

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