Home
Product
Protein
Recombinant Proteins
Recombinant Xenopus laevis Serine/threonine-protein kinase PLK1 (plk1)

Recombinant Xenopus laevis Serine/threonine-protein kinase PLK1 (plk1)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

plk1

Uniprot NO.

P70032

Alternative Names

plk1; plx1; Serine/threonine-protein kinase PLK1; EC 2.7.11.21; Plx1; Polo-like kinase 1; PLK-1

Species

Xenopus laevis (African clawed frog)

Source

Yeast

Expression Region

1-598

Target Protein Sequence

MAQVAGKKLT VAPEAAKPPG IPGSSSAVKE IPEILVDPRT RRRYLRGRFL GKGGFAKCYE ITDLESREVF AGKIVPKTML LKPHQKDKMT MEIAIQRSLD HRHVVGFHGF FEDNDFVYVV LELCRRRSLL ELHKRRKAVT EPEARYYLKQ TISGCQYLHS NRVIHRDLKL GNLFLNDEME VKIGDFGLAT KVEYDGERKK TLCGTPNYIA PEVLGKKGHS FEVDIWSIGC IMYTLLVGKP PFETSCLKET YMRIKKNEYS IPKHINPVAA ALIQKMLRSD PTSRPTIDDL LNDEFFTSGY IPSRLPTTCL TVPPRFSIAP STIDQSLRKP LTAINKGQDS PLVEKQVAPA KEEEMQQPEF TEPADCYLSE MLQQLTCLNA VKPSERALIR QEEAEDPASI PIFWISKWVD YSDKYGLGYQ LCDNSVGVLF NDSTRLIMYN DGDSLQYIER NNTESYLNVR SYPTTLTKKI TLLKYFRNYM SEHLLKAGAN TTPREGDELA RLPFLRTWFR TRSAIILHLS NGTVQINFFQ DHTKIILCPL MAAVSYIDEK REFRTYKLSL IQEFGCCKEL ASRLRYARTM VEKLQSSKSA VAHVKASA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates cdc25, pkmyt1/myt1, stag2/sa2, tpx2. Plays multiple essential roles during mitosis. Phosphorylates the N-terminal domain of cdc25, which leads to cyclin b-cdc2 activation and mitotic entry. Also required for organization of bipolar spindles, and for exit from mitosis. Involved in kinetochore functions and sister chromatid cohesion by phosphorylating stag2/sa2.

Gene References into Functions

Novel roles for Plk and GSK3 regulation of ADAM13 function in cranial neural crest cell migration. PMID:25298404
Plx1-mediated degradation of Bora in interphase generates oscillations in Plx1 activity and is essential for development. PMID:24675888
Coordinated interplays between Plx1 and Gwl are required for reactivation of these kinases from the G(2)/M DNA damage checkpoint and efficient checkpoint recovery. PMID:21708943
the penultimate CRS serine (Ser 101) of cyclin b1 is a Plx substrate PMID:12612056
Results suggest that polo-like kinase (Plx1) could be the missing regulator that prevents maturation-promoting factor autoamplification in stage IV oocytes. PMID:14985258
Plx1 cooperates with CaMKII to regulate cyclosome regulators, and is necessary for release of cytostatic factor metaphase arrest and sufficient when overexpressed. PMID:16040245
Data show that Plx1 couples tension signals to cellular responses through phosphorylation of the 3F3/2 epitope and targeting structural and checkpoint proteins to kinetochores. PMID:16129782
Polo-like kinase Plx1 is an essential factor for calcium ion-induced meiotic exit during cytostatic factor arrest PMID:16407128
Polo-like kinase (Plx1) phosphorylates xTRF1 in vitro and the mitotic xTRF1-chromatin association was significantly impaired when Plx1 was immunodepleted from the extracts (Plx1). PMID:16424898
Cdk1 phosphorylation of BubR1 controls spindle checkpoint arrest and plk-mediated formation of the 3F3/2 epitope. PMID:17998400
Plx1 promotes activation of Aurora A, most likely through TPX2. PMID:19556869