Recombinant Xenopus laevis Protein Wnt-5a (wnt5a)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

wnt5a

Uniprot NO.

P31286

Species

Xenopus laevis (African clawed frog)

Source

Yeast

Expression Region

41-380

Target Protein Sequence

VVESSSWWSL GMNPVQMPEV YIIGAQPLCS QLSGLSQGQK KLCQLYQDHM QFIGDGAKTG IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA ISAAGVVNAV SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNLDYGY RFAKEFVDAR EREKIHQKGS YESSRIMMNL HNNEAGRRAV STLADVACKC HGVSGSCSLK TCWLQLADFR KVGDHLKEKY DSAGAMKLNT RGKLVQVNNK FNSPTMNDLV YIDPSPDYCV HNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV QTERCHCKFH WCCYVKCKKC TEVVDQFACK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. Plays a role in normal embryonic development.

Gene References into Functions

In midgastrula embryos, Wnt5a, Wnt11, and Wnt11b, but not Wnt3a, acted across many cell diameters to orient Prickle3/Vangl2 complexes away from their sources regardless of their positions relative to the body axis. PMID:27658614
Xenopus adult stem cells originate from the larval absorptive cells expressing Ror2, which require Wnt5a/Ror2 signaling for their dedifferentiation accompanied by changes in cell morphology. PMID:25211363
Data show that PAPC signaling via RhoA and Wnt5a/Ror2 activity are required to keep cells aligned in apical-basal orientation during invagination of the ear placode. PMID:21663658
Data show that Rspo3 binds syndecan 4 and that together they activate Wnt5a/PCP signaling. PMID:21397842
In an examination of signaling pathways in developing Xenopus lung, wnt5a was expressed in the mesenchyme layer of the entire lungs through stages 39-41. PMID:20682360
Wnt5a reduces cell surface levels and promotes ubiquitination and degradation of SDC4 in dorsal mesodermal cells from Xenopus gastrulae. PMID:20639201
Transcriptional regulation of XPAPC by XWnt-5A requires the receptor tyrosine kinase Ror2. PMID:17488628
Xwnt-5a plays an instructive role in larval tail regeneration via Wnt/JNK signaling PMID:18977433

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Subcellular Location

Secreted, extracellular space, extracellular matrix. Secreted.

Protein Families

Wnt family

Tissue Specificity

Found primarily in ectoderm with lower levels of expression in mesoderm. Detected in the head and tail with lower expression in the middle of the embryo. No expression was found in the notochord.

Database Links

UNIGENE: Xl.19997

KEGG: xla:378689

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