Recombinant Xenopus laevis Noggin (nog)

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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

nog

Uniprot NO.

P49011

Species

Xenopus laevis (African clawed frog)

Source

Yeast

Expression Region

20-222

Target Protein Sequence

R IDQGGCQHYL HIRPAPSENL PLVDLIEHPD PIYDPKEKDL NETLLRTLMV GHFDPNFMAT ILPEERLGVE DLGELDLLLR QKPSGAMPAE IKGLEFYEGL QSKKHRLSKK LRRKLQMWLW SQTFCPVLYT WNDLGTRFWP RYVKVGSCYS KRSCSVPEGM VCKAAKSMHL TILRWRCQRR VQQKCAWITI QYPVISECKC SC

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Description

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Target Background

Function

Patterns the embryo by interrupting bone morphogenetic proteins (BMP) signaling. Binds BMP-4 and BMP-2 with high affinity. Can abolish BMP-4 activity by blocking binding to cognate cell-surface receptors. Capable of inducing dorsal development in embryos. Causes dorsal mesodermal differentiation of animal cap ectoderm when coexpressed with xWNT-8 and nuclear, sequence-specific DNA-binding protein xBRA. None of these molecules causes dorsal mesoderm formation when expressed alone.

Gene References into Functions

noggin 1, differently from noggin 2 and noggin 4, is expressed during all phases of Xenopus laevis retinal development. Gain-of-function experiments by electroporation in the optic vesicle show that overexpression of noggin 1 significantly decreases the number of bipolar cells in the inner nuclear layer of the retina, without significantly affecting the generation of the other retinal cell types. PMID:27389985
a new role for Noggin1 in determining specific anterior neural structures by the modulation of TGFbeta and SHH signaling. PMID:25913744
The data provide a quantitative basis for modeling the process of Noggin1 diffusion in embryonic tissues, considering its interaction with heparan sulfate proteoglycans. PMID:26525852
This allow one to compare the expression levels of Noggin1 and Noggin 2 constructs, to purify them on the affine immunosorbent and to show the activity of Noggin proteins by analyzing their ability to bind BMP4 factor PMID:23964527
Both Noggin proteins could induce a secondary head, including the forebrain. During normal development, Noggin1 mRNA was translated with low efficiency, providing sufficient Noggin1 only for antagonizing Bone morphogenetic protein. PMID:22071106
A 2066 bp noggin 5' flanking sequence which recapitulates the roof-plate expression of endogenous gene in transgenic frog tadpoles has been identified; this roof-plate enhancer has been mapped to a sequence as short as 79 bp. PMID:14625440
Noggin specifically blocks chondrogenic differentiation, rather than osteogenic differentiation, in mesodermal stem cell line C1 and skeletal cells. PMID:15044373
Chordin, Noggin, beta-Catenin, and Cerberus have roles in neural induction in Xenopus PMID:15138495
X-epilectin expression is down-regulated by Noggin and tBR and that this effect is inhibited by BMP4 over-expression, suggesting X-epilectin expression is mediated by the BMP signalling pathway PMID:15602698
maternal mRNA encoding noggin is enriched in animal tiers and at low concentrations in the C-tier, suggesting that the neural fates of C-tier blastomeres may be responsive to early signaling from their neighboring cells PMID:17096409