Recombinant Xenopus laevis Nodal homolog (nodal)

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MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

nodal

Purity

>85% (SDS-PAGE)

Target Names

nodal

Uniprot NO.

Q91619

Alternative Names

nodal; nr1; Nodal homolog; Nodal-related protein 1; Xnr-1; Xnr1; nr-1

Species

Xenopus laevis (African clawed frog)

Source

Yeast

Expression Region

282-406

Target Protein Sequence

NRNENHHLS IGSIPSRHVE NGKPLCRRVD MIVDFEDIGW SSWIVYPKKY NAYRCEGACP IPLNETFKPT NHAYMKSVVK LYQPERVECP LCVPVKMSPL SMLYYEGDEV VLRHHQEMIV EECGCS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Cooperation and regulatory loops of multiple nodals are essential for mesendoderm patterning in early embryos. Essential for mesoderm formation and axial patterning during embryonic development. Activates the activin-like signaling pathway to induce dorsal and ventral mesoderm in animal cap ectoderm. In addition, also dorsalizes ventral marginal zone (VMZ) tissues during gastrulation. Acts in a downstream signaling cascade via cripto and cer1 to mediate cardiogenesis in embryonic mesoderm. Directs the orientation of the left-right axis by driving the left-specific gene cascade in the left lateral plate mesoderm.

Gene References into Functions

FoxH1 and Grg4 occupy the Xnr1 enhancer, and Grg4 occupancy is dependent on the FoxH1 EH1 motif. Grg4 occupancy at the Xnr1 enhancer significantly decreases with Nodal activation or Smad2 overexpression, while FoxH1 occupancy is unaffected. These results suggest that Nodal-activated Smad2 physically displaces Grg4 from FoxH1, an essential feature of the transcriptional switch mechanism. PMID:27085753
Data suggest that Coco acts as a critical target of flow, suggesting that symmetry is broken by flow-mediated left-asymmetric release of Nodal repression at the midline. PMID:20381352
Unilateral expression of Xnr-1 in the left lateral plate mesoderm directs the orientation of the left-right axis by driving the left-specific gene cascade. PMID:16281170
asymmetric expression of Nodal-related 1 (Xnr1) begins in the posterior left lateral plate mesoderm shortly after the initiation of bilateral perinotochordal expression in the posterior tailbud PMID:16959238
Xnr1 and derriere are coexpressed in the posterior paraxial mesoderm at neurula stage, and with Coco define a posttranscriptionally regulated signaling center in the chain leading to an increased TGF-beta signal on the left side of the embryo. PMID:17239842

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Subcellular Location

Secreted.

Protein Families

TGF-beta family

Tissue Specificity

In the first phase of expression, localized to the vegetal region of the blastula. During gastrulation (stage 10.5), this expression disappears and instead becomes localized to the dorsal marginal zone, with enrichment in the organizer. During the second

Database Links

UNIGENE: Xl.1037

KEGG: xla:378543

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