Recombinant Xenopus laevis Macrophage migration inhibitory factor (mif)

Product Details

Abbreviation
mif
Purity
>85% (SDS-PAGE)
Target Names
mif
Uniprot NO.
Alternative Names
mifMacrophage migration inhibitory factor; MIF; EC 5.3.2.1; L-dopachrome isomerase; L-dopachrome tautomerase; EC 5.3.3.12; Phenylpyruvate tautomerase
Species
Xenopus laevis (African clawed frog)
Source
Yeast
Expression Region
2-115
Target Protein Sequence
PVFTIRTNV CRDSVPDTLL SDLTKQLAKA TGKPAEYIAI HIVPDQIMSF GDSTDPCAVC SLCSIGKIGG PQNKSYTKLL CDILTKQLNI PANRVYINYY DLNAANVGWN GSTFA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function(From Uniprot)
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. Required for normal neural development during embryogenesis.
Gene References into Functions
  1. MIF was found to be essential for axis formation and neural development of Xenopus embryos. PMID:15024012

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Subcellular Location
Secreted. Cytoplasm.
Protein Families
MIF family
Tissue Specificity
Ubiquitous. Detected throughout embryogenesis. Detected in adult spleen, liver, kidney, heart, brain, intestine, testis and lung.
Database Links

UNIGENE: Xl.30778

KEGG: xla:399041

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