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Recombinant Xenopus laevis DNA-dependent protein kinase catalytic subunit (prkdc), partial

Recombinant Xenopus laevis DNA-dependent protein kinase catalytic subunit (prkdc), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Target Background

Product Details

Abbreviation

prkdc

Purity

>85% (SDS-PAGE)

Target Names

prkdc

Uniprot NO.

Q9DEI1

Alternative Names

prkdc; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; EC 2.7.11.1

Species

Xenopus laevis (African clawed frog)

Source

Yeast

Expression Region

1-4146

Target Protein Sequence

MLLAGAGGSS GDGQDGVASG PAESAAGILG RLHQLHGLLS LESGVGAEGA HSLLHNLAEE CLVSALGSSA LDLNTSLIFS KEFGLLAFVR KSLSSDEFKD CREEALKFLY TFLEKIGSNV QPYAMDIKTL CVIVYTKDRA AKCKIPSLEL LIKLLQLLKN SIIIEEFKIG EIFNKFYGEL ATKSKLSDTV LEKVYELLGI LGEVQPCEMT YNSEKLFKAF LGELKAQMNS STRNPKFPVI AGCLKGLSAL MINFTKTMEE DPRTSKEIFD YTVKAISPQV EMKRYAVPSA GLNLLALHAS QFSSYLMDDY QSLFEVISKW CGHTNGEMKK LAFAALDSFL KQIAHLVASD AETHKNKLHF FMEQFYEIIR KMDSSNKELS IAIRGYGLFA APCKAVNAKN VDLMYIELIQ RCKQMYLTEA DTEEDNVYQL PNFLQSVASV ILHMDSIPEV YTPILERLLV VQIDSFPQYS LKMQSSCCKA VLKVFLSLAG KGPVLWSLIS TVVHQGLIRV CSKPVVLAQD GKEGSEAETA AATGEVRAGK WKVPTYKDYL DLFRNLLRCD QLKDSIFSDE IFSTVNSPLQ SLNRLLYDEL MKSILKIIEK LDLSLQKQDT GQEDDGGINN LLINATSDPA GNLYATKPKD FTAFVNLVEF CSEILPKEHI EYFESWVYVF GYELVLQSTR LPLISGFYKL LSVVMKNAKK SRYFEGFTSK IYKKAPEDPE RLSCFALFAK FGKEVSSKIR QFKDELLASC LTFVLHLPHD IIMMDIKAYI PALQTAFKLG LSCPPLADVG LNALQYWSTN IPSDILKPYY KDIIPLLDGY LTNLSSTNES LSTLDMVRIS RSLHKGFNKQ LIQQLKRMKT LSVKEESSLT AVRNRVVRIL GSLGGQINRS LVTAASTEEM IKRHVSWDTE KRLRFDVPFK DLKPVIYLDM FLPHITELAL STSDRQTKVA ACELLHSIVA FMLGKATQMP DDKKTGSSPM YKIYKRTFPV LLRLACDVDK VTEQLYKPLV MSLIHWFTNN KKFESQDTVA LLEAILTGIV DPVDSTLRDF CGQCIQEFLR WSIKQTTPDQ QAKSPVNTTS LFKRLYSLAL HPNAFKRLGA ALAFNNIYRD FREETALVEN FVFEVLVIYM ESLALSHADE KSLGTTQQCS DAVDHLKRII IRKAASLNKA TKRRIPRGFP QGNTVCLFDI VLWLLEQCGR PQTECRHKAM QLFFEFVPLL PGNKPLTAWL DDQVEKEGII FLINRFEGAG HSDGMHTGIF NIPALHDLHE PFSMHAVLQW LDMLLAALDC YNTFIGMRFL KANTVLGKNA EKSSFLKAAF FFITSLSMEN IKAAEQCMGS KSSVFSPHEI EAYNYSKCTI IVRIMEFITM FIDICQQDSL KILENSVFNE PMWELIAITV CDPSSIGFNT ADVEVINNLP NICIKLMKAL GNTSYRSSLE VSLKKRVTLQ SIEELCSVDL YDPGARFNRV KLGSVLSACK QLYKAEFFNS IVPEQVGGQR FGSKLLSVVY KGIAPTNERK SLPSLDISSK RLAEGLLELA FMFGGQCEEL VSLLLNTVIL SVPLPGTSQR NIINFSHGGY FYTLFAETIN TELLNNLDTI VVELMKSSLE DPKMVSCVLN GMLDQSFRQR TIRKQQGVKL VNAVLENWRR LDSWWYKDSP SESKMAVLTL LAKVLQIDSS VCFDINHSAF AEVFKTYTSI LTDQKLGLNL KSQAIIILPF FTKLTGEKLT ELKNTLDQFV ASNFPMKSDE FPKGTLKFNN YVDCIKKFLD ALELSQSPML LQLMTEILCR DERHFMEELF QSSFKKVIKR SSCDTQVILL NTLHNMFKSE SLMLNGTLQS LIDRCLLTLL WNCSLDAMIS FFTNIISLAM DTLKSRFTKV PEAAFDSQIT KKWGYYKMLE VQYSRLSKDE IYSTVNNAYH VSSKPEGNEL TKALIKLCYD TFTENMCGET QLLEKRRQYH CAAYNCAISL ISCVFSELKF YQGFLFTEKK EKNLLIFENL IDLQRNYTFP IEVEVPMERK KKYFAIRKEA RDASSTESDE PSYLSSQSYM ADSSLIEEMS QFDFSTGVQS FSYSSQSKML SQSASRKKEQ ITEGKTFDDV MEFEMDELNQ HECMAAMTGL IKHMNRSEIT PKVDEDASPQ ELPSWMKFLH VKLGNTSTPL NIRLFIAKLI VNTEEVFRPY ARFWIGPILQ LIVSGNNGGT GIHYMVVETL VTILSWSSIA TPNGQAKEEI LANRLLEFLM KNVFHEKRAV FRHNLEIIKT VLECWKECLS IPYRLIYEGF SGTDPNTKDN SVGIQLLGLA LANNFSPLDP KCGIDPERYF QSLANNLGLT RFKEVYIAAA EVIGLVLRYI VQNEKRTEAP VFDYVVKELK RHQTNNKEDK FIMCLNKVVK NFPPFADRFM TIVLFLLPKL HGVLKTQCLE IIMHRAEDIP DLFIELKNKD FCQIMNNRDD ERQRVCLDII YKILSKLTPA ELHEFLIPVT AFSSHSFPVC RERMYDIFMW IYDNYRDHES QNDSKSVEVF NMAKEGLLQG LVDENTELQL IVRNFWSDET RLPSNTTERM LAILSSLYSP KIEKHYLSLA TNLLLEMTSK SPDYIRKMFE HPLSECKFQD YTVDSSWRFR SSVLTPMFVE TQLSQSMQRS RAQGTIEADE PIGGQLRATQ QHYQFTPTQN IGGRSSFNWL TGSSMDTLAD YSVESPESLP SALLFVNKRN ENVRRVPLKP LGPNFGMRRL GLPGDVTDSK TKSMEDRSDI LRLRRRFLKD REKLSLIYAR KGTAEQKREK AIKIEQKMKQ DAQITLYRNY RQGELPDIQI SYSNLIAPLQ ALAQRDPTMA KLLFSSLFSG ILTDTASDIS VTDKLLKQFN SFLSNSLSYF PPFIACVQDM CYQHDELLHL NPANISTSCL ASLQQPLGIL LLEKGLLHMK VPDEPPAKKM RKEKEKAEIP PDIVRWIELA KLYRSIGDYD VLRGIFSGKI GAKSITQCAL NAEAKSDYAK AAKLYDEALT ETFSDGDPTD AEKDFWELAS LECYNHLTEW KPLEYCSTVN IDTGKPPDLN KMWSDPFYQE TYLPYMIRSK LKMLLGGNND QTLLTFVDEA MKVEQRKVLM ETFYSQELSL LYILQDDFDR AKYYINNGIQ VFMQNYSSID CLLYQSRLTK LQSVQALTET QDFISFIRKP GNVSSSSLRK LFQGWMKRYP DSKMDPMNIW DDIISNRCFF LDKIQDVAVG HPQLVDESME VDDLADGNEA MEVDRQEDIA VMINKCRFTM KMKMVDSARK QNNFSVAMKL LKDLHRESKT NEDWSVKWIH SYSRYSHSRS RDLTCSEQIL TALKTIPLLE ESKTEYLTKN TKACRYQNML LGDTYRIMAD AVCKEPDCLY KIEDGKAGKV KDLSESPENV VGGLYRKSLH YFTNAVRKAT EEEQSHSTDQ IDVRGIIKAY MTLVDFCDSH LRKVEEESAV MDRADYQNFP EIMVEKMIKA LKLNSSEARL KFPRMLQIIE QYPSETLDLM ARENCTVPCW QFIGWISQMM AMLDKKESIA VQHIIEEIAE NYPQALVYPF MVSGESYNFE DTVVGHKNRE YVNRIKSKLD KDNVAQDFIR ALEQLSNPPM IFQDWWEDVS NELSKPNVNK NKIKELYKEM YTNLGNPKDH FMGAFRRRFC EKYTKDFDKA FGPEGSKLLN IKCDGFNKTV GPLITKMKEQ QKEPGNLKEY SPWMSEFKPE FLRNELEIPG QYSGRSKPMP EYHVKISGFD ERVSVMASIR KPKRIIVRGN DEREYPFLVK GGEDLRQDQR IEQLFEIMNI ILSQDAACSQ RHMQLKTYQV IPMTTRIGLI EWLENTCTLK EFILNTMTED EAKIYNSKTT NGPLYHYNAW LDKKEKVGDA RQHVTSYTRC DRTNTVASFR EREALVPKDL LRRAFVKMST TPEAFLSLRS HFARSHALLC VSHWIVGIGD RHLSNFMINM ETGGMIGIDF GHAFGTATQF LPVPELMPFR LTRQIVNLML PMKDSGLFDS VMVHSLRAYR SDPGLLVTTM DVFIKEPSLD WKNLELKQMK KKGEWKKAVD VTSHNWHPQQ KIHCAKRKLD GANPCEITCE ELRLGHESAP EYKDFIAVAR GDKKHNRRTN EPPDGLTEET QVQCLIDQAT DPNILGRVWK GWEPWI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). Recruited by XRCC5 and XRCC6 to DNA ends and is required to (1) protect and align broken ends of DNA, thereby preventing their degradation, (2) and sequester the DSB for repair by NHEJ. Act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX, thereby regulating DNA damage response mechanism.

Gene References into Functions

Ku80 is susceptible to proteolytic degradation when not dimerized with Ku70; dimer induces DNA-PK activity; evidence that interacting domain(s) of Xenopus DNA-PKcs is conserved so as to interact with human Ku, reconstituting a functional holoenzyme PMID:20047046
These results suggest that potential mechanisms occurring outside of the nucleus might activate DNA-PK, and therefore, could reveal novel functions of this enzyme. PMID:17973081

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Subcellular Location

Nucleus. Nucleus, nucleolus.

Protein Families

PI3/PI4-kinase family

Database Links

UNIGENE: Xl.391

KEGG: xla:373602

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