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Recombinant Ustilago maydis DNA topoisomerase 1 (TOP1), partial

Recombinant Ustilago maydis DNA topoisomerase 1 (TOP1), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

TOP1

Uniprot NO.

P41511

Species

Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)

Source

Yeast

Expression Region

1-1019

Target Protein Sequence

MNSIQVKNEP MLASFASTST NGKAKRSAKP SLFSGSEVSS DDDEEKPLAK RPKVEDSDSD APLTSTVSSQ NGVQKRSGSS NNDDNDDDSD SDSDAPLTAL VKKSNGSDDD EDDDDDDDEG DDDDEEDDDD DDDDDDKPLS KSSKSNRKPP KTMSITGSGE KKWDVLIHKG PRFPDPYQPL PKDVKLKYDG RPVDLPWQTE EIAMFYAVKL ETQHAQNAIF NRNFFDDFKT DLKKYPPRDG TQIKSFDKLD FRDMYNYWRS LKDAELERKK ALAPSARKRE IEERKAEETK WKICLVDGVE QRVGNVNVEP PGLFLGRGAH PKAGKVKRRI SPGDITINHS ADHPAPQPPA GMGDWAEVVE KKDVTWLAYW KENINGQYKY VFLDATSNFK TNSDREKFEK ARKLDTVVKQ IRRDVNKNLK SKVRHERQIA TIVWLIDNFS LRAGNEKGED EAETYGVCSL RCEHAQIKMP DTIHLEFLGK DSMKFEEDLK ITNPDVFKNI AMFLKSNGMK DKSGNYVRKK PSDPIFCAPE SGSGKMQPLQ PNSVNQFLSK YMKGLSAKVF RTYNASVTFQ GLLEQTEEWL KSRPNAAERE INQTNLRLAY NEANRQVAIL CNHQKTVNPM LLNRNLERTQ DKIFQIRYEI MKEQQKILTF HKVSELKKEF KVKEHPFMKQ FDKIMQKQDL DAEKVKQYEE QMISDKKSKL ESTFKRQQSE LQYQLEQKGL TGDDGTPKKG KKAKNVEEEV RSSIKGFKDK KQVDEELKAL NETAKRLEKE RKTNKSQATS CNFVSSAKKI LSKYEMIKKQ EAELVNKNNT SDVALSTSKL NYIDPRITLA WLKEWDDRLS DLGQGKAAPK KKVKKEEEEN DIKPKKKDAK GAASKKRAAK TGLANSTGDS EKMELGLQVM NISQFFANAL QKKFKWAASG DDGRDISAKW VFVKDAQSKM RKLDSAERKG QKGGSMAAMT DAADSKEAQP KVNCVLKKQT SADRKMSKPI KAVDKTEESD DDLSSDSSDD EDKPLAAVV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Protein Families

Type IB topoisomerase family

Database Links

KEGG: uma:UMAG_05101

STRING: 5270.UM05101P0

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