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Recombinant Sulfolobus acidocaldarius DNA double-strand break repair Rad50 ATPase (rad50), partial

Recombinant Sulfolobus acidocaldarius DNA double-strand break repair Rad50 ATPase (rad50), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

rad50

Uniprot NO.

O33600

Alternative Names

rad50; Saci_0051; DNA double-strand break repair Rad50 ATPase

Species

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

Source

Yeast

Expression Region

1-886

Target Protein Sequence

MIIREIRLQN FLSHEDTTVK FEGSINVIIG NNGAGKSSII DGILFGLFKR TNRDIGKNEE LIKKGKKSGQ VSIKFEINGD TYLIDRNVGE TSRDTISLLK EGKIITLARQ STTVNNKIKE ILGFDHKILM STTIIGQGSV ESVFSDFPEV MKELLKINKL EMLRESNGPI HSLIKVLTDR IRSLQSIKDI LKREEAEIDR LKKEIEEIKV KLENIEREAK EKEDELNQYN TEFNRIKEIK VQYDILSGEL SVVNKKIEEI ALRLKDFEEK EKRYNKIETE VKELDENREK INTISSFKSI LVQIDSLKSQ INVVENDLKR KKEKLKRKKE LEEKEKQYEE IEKRKKELEE KEKQYEEIEK RLTYVLKNIE RQKNEIEKLN YVDTQDLENK IKDVSDRINQ IDNELKGLLD RRGDLNGRKE QTLKIYNNLN SIEDDRCPIC GRPLDSEHKA KIREEIKVQL LELNKQITAL QARINSLIKE REELEATRNK LQLELQKRSK EKGIYEAKLK ELQRLEEEKN KLQNEILSLL SYHQEFENIA EKEKELIDYH EEYLKNSDIL EEDIQEQEQR LNELNSKLSE LEKSYNDYKA KYQFLPADLK SLVSLEERIR RRISELEKLK IEYERLKEEI TRMKGLKEEY EKLKEEEDAL LNRISELGYS EKRYKQLEEI IDKLSKILSG IEADKGKIKG SLEEKIKNIE EKERNIEELR NKMNEESKLN LGISKLQKLR EVLDNKHLQS HIMNIVRNQI ENNVNEVIAK FDLSFSAVEI DFVGKSELYV YTASGQKIHI NALSGGERIS IALALRLAIA KALMNQFSTL ILDEPTVNLD EYRRKELIDV IRSAIEIVPQ IILVTHDQEL IQAGDYIIRV EKKGDTSKVE VSSYDR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.

Gene References into Functions

These results demonstrate the functional interaction between Mre11, Rad50 and the HerA helicase and suggest that each protein play different roles when acting on its own or in association with its partners. PMID:18294364

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Protein Families

SMC family, RAD50 subfamily

Database Links

KEGG: sai:Saci_0051

STRING: 330779.Saci_0051

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