Home
Product
Protein
Recombinant Proteins
Recombinant Schizosaccharomyces pombe DNA topoisomerase 1 (top1), partial

Recombinant Schizosaccharomyces pombe DNA topoisomerase 1 (top1), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

top1

Uniprot NO.

P07799

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Source

Yeast

Expression Region

1-814

Target Protein Sequence

MSSSDSDSVS LSIRRRQRRG SSKRISMKES DEESDSSENH PLSESLNKKS KSESDEDDIP IRKRRASSKK NMSNSSSKKR AKVMGNGGLK NGKKTAVVKE EEDFNEIAKP SPKHKRVSKA NGSKNGAKSA VKKEESDTDD SVPLRAVSTV SLTPYKSELP SGASTTQNRS PNDEEDEDED YKWWTSENID DTQKWTTLEH NGVIFAPPYE PLPKNVKLIY DGNPVNLPPE AEEVAGFYAA MLETDHAKNP VFQDNFFRDF LKVCDECNFN HNIKEFSKCD FTQMFHHFEQ KREEKKSMPK EQKKAIKQKK DEEEEKYKWC ILDGRKEKVG NFRIEPPGLF RGRGSHPKTG SLKRRVYPEQ ITINIGEGVP VPEPLPGHQW AEVKHDNTVT WLATWHENIN NNVKYVFLAA GSSLKGQSDL KKYEKSRKLK DYIDDIRKGY RKDLKSELTV ERQRGTAMYL IDVFALRAGN EKGEDEADTV GCCSLRYEHV TLKPPRTVVF DFLGKDSIRY YNEVEVDPQV FKNLKIFKRP PKKEGDLIFD RLSTNSLNKY LTSLMDGLSA KVFRTYNASY TMAEELKKMP KNLTLADKIL FYNRANRTVA ILCNHQRSVT KNHDVQMERF AERIKALQYQ RMRLRKMMLN LEPKLAKSKP ELLAKEEGIT DSWIVKHHET LYELEKEKIK KKFDRENEKL AAEDPKSVLP ESELEVRLKA ADELKKALDA ELKSKKVDPG RSSMEQLEKR LNKLNERINV MRTQMIDKDE NKTTALGTSK INYIDPRLTY SFSKREDVPI EKLFSKTIRD KFNWAADTPP DWKW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. TThe free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Gene References into Functions

SUMOylation and SUMO-dependent ubiquitylation by the Pli1-Slx8 axis contribute in different ways to maintain genome stability PMID:23936535
Topoisomerase I activity is directly required for efficient nucleosome disassembly at gene promoter regions. PMID:20526281

Hide All

Protein Families

Type IB topoisomerase family

Database Links

KEGG: spo:SPBC1703.14c

STRING: 4896.SPBC1703.14c.1

Promotions