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Recombinant Saccharomyces cerevisiae Suppressor of kinetochore protein 1 (SKP1)

Recombinant Saccharomyces cerevisiae Suppressor of kinetochore protein 1 (SKP1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SKP1

Uniprot NO.

P52286

Alternative Names

SKP1; CBF3D; YDR328C; D9798.14; Suppressor of kinetochore protein 1; Centromere DNA-binding protein complex CBF3 subunit D; E3 ubiquitin ligase complex SCF subunit SKP1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Source

Yeast

Expression Region

2-194

Target Protein Sequence

VTSNVVLVS GEGERFTVDK KIAERSLLLK NYLNDMHDSN LQNNSDSESD SDSETNHKSK DNNNGDDDDE DDDEIVMPVP NVRSSVLQKV IEWAEHHRDS NFPDEDDDDS RKSAPVDSWD REFLKVDQEM LYEIILAANY LNIKPLLDAG CKVVAEMIRG RSPEEIRRTF NIVNDFTPEE EAAIRRENEW AEDR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Essential component of the E3 ubiquitin ligase complex SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins like phosphorylated SIC1. Participates in the attachment of chromosomes to the spindle. Acts as a regulatory component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromeres and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly. SKP1/CBF3D could retrieve cyclins or cyclin-CDK-like proteins into the kinetochore thus providing cell cycle-regulated kinetochore activity. Involved in the regulation of methionine biosynthesis genes. Facilitates association of CDC53 with CDC4 and of ROY1 with YPT52.

Gene References into Functions

findings revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1; this repression appeared to be mediated through the ROK1 uORF1 PMID:26310304
Data show that the amino terminus of Sgt1p interacts with CBF3 subunits Skp1p and Ctf13p. PMID:15456868
Sgt1p acts as a "client adaptor" recruiting specific clients to Hsp82p co-chaperone complexes PMID:16945921
Skp1p does not appear to be involved in V-ATPase assembly but instead in the cycling of the complex off membranes PMID:17041187
findings suggest a novel function of Skp1 in mitotic exit and cytokinesis, independent of its function as a part of the SCF complex; interaction of Skp1 and Bfa1 may contribute to the function of Skp1 in the mitotic exit PMID:17205042
Saf1p is being a bona fide Skp1-Cullin-F-box subunit PMID:17517885