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Recombinant Saccharomyces cerevisiae Phosphoenolpyruvate carboxykinase [ATP] (PCK1), partial

Recombinant Saccharomyces cerevisiae Phosphoenolpyruvate carboxykinase [ATP] (PCK1), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

PCK1

Uniprot NO.

P10963

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Source

Yeast

Expression Region

1-549

Target Protein Sequence

MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN KTVISSSGAL IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT WSINRERAAD YLRTRDHIYI VDAFAGWDPK YRIKVRVVCA RAYHALFMTN MLIRPTEEEL AHFGEPDFTV WNAGQFPANL HTQDMSSKST IEINFKAMEM IILGTEYAGE MKKGIFTVMF YLMPVHHNVL TLHSSANQGI QNGDVTLFFG LSGTGKTTLS ADPHRLLIGD DEHCWSDHGV FNIEGGCYAK CINLSAEKEP EIFDAIKFGS VLENVIYDEK SHVVDYDDSS ITENTRCAYP IDYIPSAKIP CLADSHPKNI ILLTCDASGV LPPVSKLTPE QVMYHFISGY TSKMAGTEQG VTEPEPTFSS CFGQPFLALH PIRYATMLAT KMSQHKANAY LINTGWTGSS YVSGGKRCPL KYTRAILDSI HDGSLANETY ETLPIFNLQV PTKVNGVPAE LLNPAKNWSQ GESKYRGAVT NLANLFVQNF KIYQDRATPD VLAAGPQFE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Gene References into Functions

the electrostatic interaction between the guanidinium group of Arg70 and the carboxyl group of PEP is important for PEP binding by phosphoenolpyruvate (PEP) carboxykinase and for further steps in catalysis. PMID:15358051
neighboring Phe(416) contributes to provide a low polarity microenvironment suitable to keep the epsilon-NH(2) of Lys(213) in the unprotonated form PMID:16469427
Introduction of a hydroxyl group in the lateral chain of the residue (Phe216Tyr)produces a substantial loss in the enzyme affinity for Mn(2+). PMID:17195942
Arg457 is an important factor for nucleotide binding by S. cerevisiae PEP carboxykinase. PMID:18346928
Functional evaluation of serine 252 of Saccharomyces cerevisiae PCK1 is reported. PMID:18996167