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Recombinant Saccharomyces cerevisiae Nitrogen permease reactivator protein (NPR1), partial

Recombinant Saccharomyces cerevisiae Nitrogen permease reactivator protein (NPR1), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

NPR1

Uniprot NO.

P22211

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Source

Yeast

Expression Region

1-790

Target Protein Sequence

MSSLTRLLQE KRKNETSNSS PRTSADTLTT TPESQSLDLH SRNKSSSHIG SVSNSSSSDR NRANVPVPGS VTTVTQIYSE EDSSSTAGSS LDDRNQFSSS FLNANFAHTA SFYGTSAQSR DRFGSLINDQ GTAGLSSHGG SFAAQNRITS RLSTTSHTSG RAIPSLSSSI PYSVPNSNKD NNSSNSNSSS LSSSWLETYA GGMPNNISAI DSNVISSPKV DSVEPRFVIS KQKLQKASMD SNNANATQSR SISRSGSFSS QLGNFFFSKN SKESSNSNSA GMSFSANSNG PSPNIKNPNV TNGSTPIPKP IRARQSSIYS ASRQPTGSYT DNFYGSPSSV HDHLPPSQSV PRSQHSSIGD LKRFFKKSSN SNLSSNSNNV IPNGSPLSSG IAVPSHSHSS SHFAAGNNSY STSYNGNGDT IYSHSHGGSG IPFSKRYIKT GADLGAGAGG SVKLAQRISD NKIFAVKEFR TKFENESKRD YVKKITSEYC IGTTLNHPNI IETIEIVYEN DRILQVMEYC EYDLFAIVMS NKMSYEEICC CFKQILTGVQ YLHSIGLAHR DLKLDNCVIN EKGIVKLIDF GAAVVFSYPF SKNLVEASGI VGSDPYLAPE VCIFAKYDPR PVDIWSSAII FACMILKKFP WKIPKLRDNS FKLFCSGRDC DSLSSLVTRT PDPPSYDESH STEKKKPESS SNNVSDPNNV NIGPQRLLHS LPEETQHIVG RMIDLAPACR GNIEEIMEDP WIRSIDMCHL VEDGLSFKVV RGEDHHHTQV DQSEAHIAGL EKKKKKQNNQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Nutrient-regulated protein kinase that promotes the activity of at least 6 distinct transport systems for nitrogenous nutrients under conditions of nitrogen catabolite derepression. Under poor nitrogen growth conditions, required for post-Golgi sorting of the general amino acid permease GAP1 and the three known ammonia permeases, MEP1/2/3, to the plasma membrane. Contributes also to the stability and the retention of GAP1 at the plasma membrane. Inversely, promotes the degradation of tryptophan permease TAT2 under the same conditions. Activity is regulated by the TOR signaling pathway via phosphatase SIT4. Although thought to be involved in regulation of GLN3-dependent transcription by nitrogen catabolite repression, this seems to be an indirect effect from the reduced uptake of the nitrogen-repressing compound.

Gene References into Functions

Study shows that Npr1 inhibits the activity of Art1, an arrestin-like adaptor protein that promotes endocytosis by targeting the Rsp5 ubiquitin ligase to specific plasma membrane cargoes. PMID:22118465
the connection between Gln3 localization and Npr1 is indirect, arising from the influence of Npr1 on the ability of cells to utilize ammonia as a repressive nitrogen source. PMID:16864577
The growth impairment of cells lacking Npr1 on many nitrogen sources is shared by cells deprived of the three Mep proteins and is a consequence of deficient ammonium retrieval. PMID:17493133
Siw14 controls the intracellular localization of Gln3 in combination with Npr1, and one of the causes for the caffeine sensitivity of the Deltasiw14 disruptant was an accumulation of dephosphorylated Gln3 in the nucleus. PMID:18166280