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Recombinant Saccharomyces cerevisiae E3 SUMO-protein ligase MMS21 (MMS21)

Recombinant Saccharomyces cerevisiae E3 SUMO-protein ligase MMS21 (MMS21)

Code
MSDS	MSDS
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

MMS21

Uniprot NO.

P38632

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Source

Yeast

Expression Region

1-267

Target Protein Sequence

MALNDNPIPK SVPLHPKSGK YFHNLHARDL SNIYQQCYKQ IDETINQLVD STSPSTIGIE EQVADITSTY KLLSTYESES NSFDEHIKDL KKNFKQSSDA CPQIDLSTWD KYRTGELTAP KLSELYLNMP TPEPATMVNN TDTLKILKVL PYIWNDPTCV IPDLQNPADE DDLQIEGGKI ELTCPITCKP YEAPLISRKC NHVFDRDGIQ NYLQGYTTRD CPQAACSQVV SMRDFVRDPI MELRCKIAKM KESQEQDKRS SQAIDVL

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Description

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Target Background

Function

Acts as an E3 ligase mediating SUMO/Smt3 attachment to SMC5 and YKU70. Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.

Gene References into Functions

Mms21 prevents the accumulation of spontaneous DNA lesions that cause diverse gross chromosomal rearrangements. PMID:29505562
Data indicate that Snf1 Is the target of SUMO (E3) ligase Mms21. PMID:24108357
Deletion of E3 ligase domain of Mms21 led to decreased binding of Sir2p, Sir3p and Sir4 at subtelomeric chromatins and increased H3K4 tri-methylation at telomere-distal euchromatin regions. PMID:23911609
Small ubiquitin-related modifier ligase activity of Mms21 is required for maintenance of chromosome integrity during the unperturbed mitotic cell division cycle in Saccharomyces cerevisiae PMID:21324902
Mms21 requires the Siz/PIAS (protein inhibitor of activated signal transducer and activator of transcription) RING domain to function as a SUMO ligase. PMID:15738391
Molecular cloning proved pso10-1 to be a mutant allele of gene MMS21 that encodes a SUMO ligase involved in the sumoylation of several DNA repair proteins. PMID:18437386