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Recombinant Saccharomyces cerevisiae DDK kinase regulatory subunit DBF4 (DBF4), partial

Recombinant Saccharomyces cerevisiae DDK kinase regulatory subunit DBF4 (DBF4), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

DBF4

Uniprot NO.

P32325

Alternative Names

DBF4; DNA52; YDR052C; D4205; YD9609.07CDDK kinase regulatory subunit DBF4; Dumbbell forming protein 4

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Source

Yeast

Expression Region

1-704

Target Protein Sequence

MVSPTKMIIR SPLKETDTNL KHNNGIAAST TAAGHLNVFS NDNNCNNNNT TESFPKKRSL ERLELQQQQH LHEKKRARIE RARSIEGAVQ VSKGTGLKNV EPRVTPKELL EWQTNWKKIM KRDSRIYFDI TDDVEMNTYN KSKMDKRRDL LKRGFLTLGA QITQFFDTTV TIVITRRSVE NIYLLKDTDI LSRAKKNYMK VWSYEKAARF LKNLDVDLDH LSKTKSASLA APTLSNLLHN EKLYGPTDRD PRTKRDDIHY FKYPHVYLYD LWQTWAPIIT LEWKPQELTN LDELPYPILK IGSFGRCPFI GDRNYDESSY KRVVKRYSRD KANKKYALQL RALFQYHADT LLNTSSVNDQ TKNLIFIPHT CNDSTKSFKK WMQEKAKNFE KTELKKTDDS AVQDVRNEHA DQTDEKNSIL LNETETKEPP LKEEKENKQS IAEESNKYPQ RKELAATPKL NHPVLATFAR QETEEVPDDL CTLKTKSRQA FEIKASGAHQ SNDVATSFGN GLGPTRASVM SKNMKSLSRL MVDRKLGVKQ TNGNNKNYTA TIATTAETSK ENRHRLDFNA LKKDEAPSKE TGKDSAVHLE TNRKPQNFPK VATKSVSADS KVHNDIKITT TESPTASKKS TSTNVTLHFN AQTAQTAQPV KKETVKNSGY CENCRVKYES LEQHIVSEKH LSFAENDLNF EAIDSLIENL RFQI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Regulatory subunit of the CDC7-DBF4 kinase, also called DBF4-dependent kinase (DDK), which is involved in cell cycle regulation of premitotic and premeiotic chromosome replication and in chromosome segregation. DDK plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. DBF4 recruits the catalytic subunit CDC7 to MCM2 and to origins of replication. DDK has also postreplicative functions in meiosis. DDK phosphorylates the meiosis-specific double-strand break protein MER2 for initiation of meiotic recombination. Interacts with CDC5 during meiosis to promote double-strand breaks and monopolar spindle orientation. Inhibits CDC5 activity during mitosis through direct binding to its PBD.

Gene References into Functions

Dbf4 interacts directly with Sld3 and promotes the recruitment of downstream limiting factors. PMID:29330352
The scaffold protein Rtt107, which binds the Mus81-Mms4 complex, interacts with Cdc7 and thereby targets Cdc7-Dbf4 and Cdc5 to the complex enabling full Mus81 activation. PMID:28096179
The Rad53-Dbf4 interaction is phosphorylation-independent and involves a novel non-canonical interface on the FHA1 domain. PMID:24285546
Dbf4 phosphorylation requires physical interaction of Dbf4 with Rad53. PMID:23564203
Dbf4 has unique fold necessary for interaction with Rad53 kinase. PMID:22130670
dbf4(7A) is a bona fide intra-S phase checkpoint bypass allele that contributes to abrogating the Rad53 block of origin firing in response to genotoxic stress. PMID:21098477
Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction PMID:21036905
Data document the role of DBF4 as a key player in nitrogen-containing bisphosphonate-induced cytotoxicity, thus explaining the effects on the cell-cycle. PMID:19744312
CDC7 and DBF4 encode the essential Cdc7-Dbf4 protein kinase required for DNA replication in eukaryotes from yeast to human. PMID:15342501
alteration of the M motif may facilitate the role of Dbf4 as a checkpoint target PMID:16107698
identified an expanded and conserved approximately 100-residue N-terminal region of Dbf4p that includes motif N but is capable of encoding a single BRCT-like domain PMID:16547092
Budding yeast Mcm4 is phosphorylated in vivo during S phase in a manner dependent on the presence of five CDK phosphoacceptor residues within the N-terminal domain of Mcm4. PMID:18321994
A complete native data set of the N-terminus of Dbf4 (motif N)was collected at 100 K from crystals that diffracted X-rays to 2.75 A resolution. PMID:19724125