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Recombinant Saccharomyces cerevisiae Autophagy protein 5 (ATG5)

Recombinant Saccharomyces cerevisiae Autophagy protein 5 (ATG5)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

ATG5

Purity

>85% (SDS-PAGE)

Target Names

ATG5

Uniprot NO.

Q12380

Alternative Names

ATG5; APG5; YPL149W; P2601Autophagy protein 5

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Source

Yeast

Expression Region

1-294

Target Protein Sequence

MNDIKQLLWN GELNVLVSID PSFLMKGSPR EIAVLRIRVP RETYLVNYMP LIWNKIKSFL SFDPLTDSEK YFWFEHNKTP IPWNYPVGVL FDCLAGKSAT FTTSFENQVK DVLTFLRIHL VMGDSLPPTI IPIASSKTQA EKFWFHQWKQ VCFILNGSSK AIMSLSVNEA RKFWGSVITR NFQDFIEISN KISSSRPRHI PLIIQTSRTS GTFRISQPTI SMTGVNPTLK DIEGDILDVK EGINGNDVMV ICQGIEIPWH MLLYDLYSKL RSFDGFLYIT LVPIKGGDKA SSEL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy). Also required for mitophagy, which eliminates defective or superfluous mitochondria in order to fulfill cellular energy requirements and prevent excess ROS production. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 and ATG8 association to the vesicle membranes. ATG12-ATG5 rearranges the ATG3 catalytic center and enhances its E2 activity. Plays a role in the regulation of filamentous growth and chronological longevity.

Subcellular Location

Preautophagosomal structure membrane; Peripheral membrane protein. Note=Localizes to the isolation membrane (IM), a membrane sac which is generated from the pre-autophagosomal structure (PAS). Ultimately, the IM expands to become a mature autophagosome. Localizes also to a dot at the junction between the IM and the vacuolar membrane, termed the vacuole-IM contact site (VICS). Correct localization to the PAS requires ATG21.

Protein Families

ATG5 family

Database Links

KEGG: sce:YPL149W

STRING: 4932.YPL149W

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