Abbreviation
Recombinant Rat Alpp protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Unit Definition:One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate(pNPP), in 1 minute at 37°C, pH10.0.The specific activity is >8000 pmol/min/ug.
Alternative Names
Alkaline phosphatase; EC:3.1.3.1; Alpp
Species
Rattus norvegicus (Rat)
Expression Region
19-501aa
Target Protein Sequence
LVPVEEENPAFWNRKAAEALEAAKKLQPIQTSANNLIILMGDGMGVSTVTATRILKGQQQGHLGPETPLAMDRFPHMALSKTYNTDKQVPDSAGTGTAFLCGVKTNMKVIGLSAAARFNQCNTTWGNEVVSVMHRAKKAGKSVGVVTTTSVQHASPAGTYAHTVNRNWYSDAHMSTAALQEGCKDIAMQLISNTDIDVILGGGRKFMFPKGTPDQEYPTDPQQAGTRLDGRNLVQEWLAKHQGAQYVWNRTELIQASLNRSVTHLMGLFEPNDMKYNIDRDPTQDPSLAEMTEVAVHMLSRNPKGFYLFVEGGRIDHGHHEAIAYRALTEAVMFDSAVDKADKLTSEKDTMIIVTADHSHVFSFGGYTQREASIFGLAPFKAGDGKSFTSILYGNGPGYKLNNGIRADVTEEESSNPTYQQQAAVPLSSETHSGEDVAIFARGPQAHLVHGVQEQNYIAHVMAFAGCLEPYTDCGLAPPAGRS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Unit Definition:One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate(pNPP), in 1 minute at 37°C, pH10.0.The specific activity is >8000 pmol/min/ug.
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The purity of Alpp was greater than 95% as determined by SEC-HPLC
Description
Alkaline phosphatase plays a central role in phosphate metabolism and serves as a widely used reporter enzyme, yet obtaining catalytically competent recombinant material with defined kinetics remains challenging for quantitative enzymology. This rat Alpp variant, spanning residues 19–501 with a C-terminal 10×His tag positioned distal to the active site, demonstrates robust catalytic function with a specific activity exceeding 8000 pmol/min/μg against p-nitrophenylphosphate at pH 10.0 and 37°C, providing a quantitative benchmark for kinetic parameter analysis including Km, Vmax, and kcat/Km determinations. The greater than 95% purity meets the quality thresholds commonly required for accurate enzyme kinetics, inhibitor screening with IC50 determination, and substrate specificity profiling where trace contaminants would confound rate measurements. Endotoxin levels below 1.0 EU/μg support use in cell-based assays for drug candidate evaluation, while the validated activity and high purity align with standards expected in structural studies by crystallography or cryo-EM.
