Recombinant Rat Wilms tumor protein homolog (Wt1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Wt1

Uniprot NO.

P49952

Alternative Names

Wt1; Wt-1Wilms tumor protein homolog

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-448

Target Protein Sequence

MGSDVRDLNA LLPAVSSLGG GGGCGLPVSG ARQWAPVLDF APPGASAYGS LGGPAPPPAP PPPPPPPHSF IKQEPSWGGA EPHEEQCLSA FTLHFSGQFT GTAGACRYGP FGPPPPSQAS SGQARMFPNA PYLPSCLESQ PSIRNQGYST VTFDGAPSYG HTPSHHAAQF PNHSFKHEDP MGQQGSLGEQ QYSVPPPVYG CHTPTDSCTG SQALLLRTPY SSDNLYQMTS QLECMTWNQM NLGATLKGMA AGSSSSVKWT EGQSNHGTGY ESENHTTPIL CGAQYRIHTH GVFRGIQDVR RVSGVAPTLV RSASETSEKR PFMCAYPGCN KRYFKLSHLQ MHSRKHTGEK PYQCDFKDCE RRFSRSDQLK RHQRRHTGVK PFQCKTCQRK FSRSDHLKTH TRTHTGKTSE KPFSCRWHSC QKKFARSDEL VRHHNMHQRN MTKLHVAL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.

Gene References into Functions

this study shows that WT1 ameliorates podocyte injury via repression of EZH2/beta-catenin pathway in diabetic nephropathy PMID:28315733
Renal WT1 expression is decreased in spontaneously hypertensive rats before the development of hypertension. PMID:27009470
High WT1 protein is associated with reduced ability to maintain podocyte integrity, rendering intrauterine growth restriction rats more susceptible for renal disease. PMID:23229934
ATRA increases the number of glomerular epithelial transition cells in experimental proteinuric glomerular diseases. Thus, ATRA may provide a useful pharmacologic approach to decipher the PMID:23107969
report of inhibition of FSHRexpression byWT1in ovarian tissue, and inhibition probably occurs via direct interaction with the FSHR promoter PMID:22431408
The re-expression of WT1 and Pax2 in the tubular epithelial cells plays important roles in the promotion of epithelial-mesenchymal transition. PMID:21778682
Downregulation of diaphragmatic WT1 gene expression may impair diaphragmatic development in the nitrofen CDH model. PMID:21072664
hypoxia upregulation of Wt1 expression involves hypoxia-inducible factor-1. PMID:12738801
Vitamin A deficiency can increase development of nephroblastomas in rats, probably via a mechanism of elevated nephrogenic rests remnants and WT1 expression PMID:16338327
genome-wide screen implicates WT1 in the transcriptional regulation of the EGF-family of growth factors as well as the CX3CL1 chemokine during nephrogenesis. PMID:17430890
Upregulation of B7.1 with the downregulation of GLEPP1, Wilms' tumor gene (WT1), megalin, and vascular endothelial growth factor started early and persisted through the course of primary glomercular disease. PMID:17457373
Wt1 modulates renin gene transcription. PMID:18496514