Recombinant Rat Thimet oligopeptidase (Thop1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Thop1

Uniprot NO.

P24155

Alternative Names

Thop1; Thimet oligopeptidase; EC 3.4.24.15; Endo-oligopeptidase A; Endopeptidase 24.15; PZ-peptidase; Soluble metallo-endopeptidase

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-687

Target Protein Sequence

MKPPAACAGD VVDTVSPCST VNHLRWDLSA QQIRALTTQL IEQTKCVYDR VGAQDFEDVS YESTLKALAD VEVTYTVQRN ILDFPQHVSP NKDIRAASTE ADKKLSEFDV EMSMRQDVYQ RVVWLQEKIP KDSLKPEAAR YLERLIKLGR RNGLHLPQDT QEKIKNIKKR LSLLCIDFNK NLNEDTTFLP FTREELGGLP EDFLNSLEKT EDGKLKVTLK YPHYFPLLKK CHVPETRRLL EEAFNCRCKE ENCAILKELV SLRAQKSNLL GFRTHADYVL EMNMAKTSQT VATFLDELAR KLKPLGEQER AVILELKEAE CAKRGLPFDG RIHAWDMRYY MNQVEETRYR VDQNLLKEYF PMQVVTRGLL AIYQELLGLT FTLEEGAAAW HEDVRLYSVR DAASGEEIGK FYLDLYPREG KYGHAACFGL QPGCLRQDGS RQLAIAAMVA NFTKPTPDVP SLLQHDEVET YFHEFGHVMH QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEKEPLMR MSQHYRTGGE APEDLLEKLI KSRQANAGLF NLRQIVLAKV DQVLHTQTDV DPAEEYARLC QEILGVPATP GTNMPATFGH LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLSPKVGMD YRTSILRPGG SEDASTMLKQ FLGRDPKQDA FLLSKGLQVE GCEPPAC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation.

Gene References into Functions

Coincubation of Kiss1 and EP24.15 demonstrated multiple cleavages of the peptide in vitro. immunohistochemistry indicated coexpression of EP24.15 and Kiss1, implicating that the regulation of Kiss1 by EP24.15 could occur in vivo. PMID:26653570
we show that Ang-(1-7) is the main metabolite of angiotensin I in rat hippocampi, and, strikingly, that thimet oligopeptidase is the main enzyme involved in the generation of Ang-(1-7). PMID:24041943
TOP changes in the brain could play important role in the balance of neuropeptide level correlated with cocaine effects. PMID:22387539
Data report mapping of the positions of the 224 surface residues that differ in sequence between thimet oligopeptidase and neurolysin onto the surface of the neurolysin crystal structure. PMID:12192079
this is the first evidence of an in vivo role for EP 24.15 in brain function, specifically in regulating alterations in microvessel permeability induced by exogenous bradykinin PMID:12586639
Analysis of transcription factors binding to promoter consensus sequences of the thimet oligopeptidase gene. Gene expression is differentially regulated in neuroendocrine and spermatid cell lines. PMID:12911328
data suggest that calcium may be an important modulator of ep24.15 cell function PMID:15955058
PTZ-induced seizures of the rats activates NMDA receptors, then decrease thimet oligopeptidase enzyme activity and immunoreactivity in hippocampal neurons. PMID:15985312
together, these data demonstrate that EP24.15/16 modulate intracellular, peptidergic signaling cascades through B2R in a physiologically relevant nociceptive system. PMID:16515556
increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load PMID:18571100