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Recombinant Rat Survival motor neuron protein (Smn1)

Recombinant Rat Survival motor neuron protein (Smn1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Smn1

Uniprot NO.

O35876

Alternative Names

Smn1; SmnSurvival motor neuron protein

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-289

Target Protein Sequence

MAMGSGGGAG SEQEDTVLFR RGTGQSDDSD IWDDTALIKA YDKAVASFKH ALKNGDMCET SDKPKGTARR KPAKKNKNQK KNATAPLKQW KAGDKCSAVW SEDGCVYPAT ITSVDLKRET CVVVYTGYGN KEEQNLSDLL SPTCEVANNT EQNTQENESQ VSTDDSEHSS RSLRSKAHSK SKAAPWTSFL PPPPPVPGAG LGPGKPGLRF SGPPPPPPPP PPFLPCWMPP FPSGPPIIPP PPPISPDCLD DTDALGSMLI SWYMSGYHTG YYMGFRQNKK EGKKCSHTN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).

Gene References into Functions

Report decreased number of motor neurons and a decrease of SMN1 protein expression in spinal cords of rats exposed to uranyl nitrate. PMID:27153795
Reduced SMN protein level alters miRNA expression and distribution in neurons. PMID:25055867
Involvement of SMN in formation of stress granules may play an important role in cell survival. PMID:21234798
RNAi-mediated degradation of endogenous Elk-1 resulted in elimination of the mcl-1 message PMID:21362474
The highest expression is associated with the cell clustering phase and expression of NMDA receptors PMID:12030329
Data show differential localization of the 18- and 23-kDa fibroblast growth factor 2 isoforms in the nuclei of rat Schwann cells, and that the 23-kDa isoform co-immunoprecipitates specifically with the survival of motor neuron protein. PMID:12397076
SMN interacts with non-methylated EWS and an EWS-derived RG-containing peptide. Symmetrical dimethylation of the EWS-derived RG-peptide increases the dissociation rate between SMN and the symmetrical dimethylated EWS RG-peptide. PMID:14597228
Data show that the rat survival motor neuron cDNA produces two isoforms with molecular weight of 32 and 35kDa, both localized in nuclear coiled bodies, but the 32kDa form is also cytoplasmic, whereas the 35kDa form is also microsomal. PMID:15465016
Forced expression of a-SMN isoform stimulates motor neuron axonogenesis in a time-dependent fashion and induces axonal-like growth in non-neuronal cells. PMID:17261814
selective overexpression of the SMN C-terminal domain promoted neurite outgrowth similar to full-length protein and could rescue the SMN knock-down effects PMID:17317728
Smn knockdown in PC12 cells alters the expression pattern of profilin II, resulting in an increase in the neuronal-specific profilin IIa isoform. PMID:17873296

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Subcellular Location

Nucleus, gem. Nucleus, Cajal body. Cytoplasm. Cytoplasmic granule. Perikaryon. Cell projection, neuron projection. Cell projection, axon. Cytoplasm, myofibril, sarcomere, Z line.

Protein Families

SMN family

Database Links

UNIGENE: Rn.1119

KEGG: rno:64301

STRING: 10116.ENSRNOP00000024456

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