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Recombinant Rat Serine/threonine-protein kinase pim-1 (Pim1)

Recombinant Rat Serine/threonine-protein kinase pim-1 (Pim1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Pim1

Uniprot NO.

P26794

Alternative Names

Pim1; Pim-1; Serine/threonine-protein kinase pim-1; EC 2.7.11.1

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-313

Target Protein Sequence

MLLSKINSLA HLRAAPCNDL HANKLAPGKE KEPLESQYQV GPLLGSGGFG SVYSGIRVAD NLPVAIKHVE KDRISDWGEL PNGTRVPMEV VLLKKVSSGF SGVIRLLDWF ERPDSFVLIL ERPEPVQDLF DFITERGALQ EELARSFFWQ VLEAVRHCHN CGVLHRDIKD ENILIDLNRG ELKLIDFGSG ALLKDTVYTD FDGTRVYSPP EWIRYHRYHG RSAAVWSLGI LLYDMVCGDI PFEHDEEIVK GQVYFRQRVS SECQHLIRWC LSLRPSDRPS FEEIQNHPWM QDVLLPQATA EIHLHSLSPS PSK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, another proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promotes cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B, induces 14-3-3 protein binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Also phosphorylates and activates the ATP-binding cassette transporter ABCG2, allowing resistance to drugs through their excretion from cells. Promotes brown adipocyte differentiation.

Gene References into Functions

Pim1 overexpression prevented NRVM apoptosis via upregulation of autophagy after hypoxia and oxidative stress, partly via activation of the AMPK/mTOR/ATG5 autophagy pathway. PMID:30257237
Pim-1 is a novel kinase that phosphorylates cTnI primarily at Ser23/24 and Ser150 in cardiomyocytes, which in turn may modulate myofilament function under a variety of physiological and pathophysiological conditions. PMID:29544221
Hypoxic preconditioning could increase short-term survival of bone marrow mesenchymal stem cells via upregulation of Pim-1, and miR-206 was one of the critical regulators in this process. PMID:27103465
Increased gene and protein expression of Pim-1 and pSTAT3 in the pulmonary vasculature of nitrofen-induced congenital diaphragmatic hernia (CDH) lungs suggest that pSTAT3 and Pim-1 are mediators of pulmonary hypertension in nitrofen-induced CDH. PMID:25812446
Pim kinases prevent premature cardiac aging and maintain a healthy pool of functional mitochondria leading to efficient cellular energetics. PMID:24916111
Nur77 is a novel negative-feedback regulator of pulmonary artery smooth muscle cell proliferation through inhibition of the signal transducer and activator of transcription 3/Pim-1/nuclear factor of activated T cells axis PMID:24047441
RAGE activation accounts for many features of Vascular remodeling diseases including VSMC proliferation and resistance to apoptosis by the activation of STAT3/Pim1/NFAT axis. PMID:21680901
Pim-1 is expressed in mesenchymal stem cells and plays an important role in the laminar shear stress-induced proliferation of mesenchymal stem cells PMID:21147132
the DE and NF-1 half-site mediate prolactin responsiveness of the pim-1 promoter PMID:12668877
Pim-1 expression was observed in the neointima of balloon-injured rat carotid arteries and in human thoracic aortas and coronary arteries showing intimal thickening suggesting that Pim-1 plays a role in vascular smooth muscle proliferation PMID:15471855
Nucleostemin expression in cardiomyocytes is induced by fibroblast growth factor-2 and accumulates in response to Pim-1 kinase activity. PMID:18519946
support a pivotal role for Pim-1 in modulation of hypertrophy by impacting responses on molecular, cellular, and organ levels PMID:18784362