Recombinant Rat SPARC (Sparc)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Sparc

Uniprot NO.

P16975

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

18-301

Target Protein Sequence

APQ TEAAEEMVAE ETVVEETGLP VGANPVQVEM GEFEEGAEET VEEVVAENPC QNHHCKHGKV CELDESNTPM CVCQDPTSCP APIGEFEKVC SNDNKTFDSS CHFFATKCTL EGTKKGHKLH LDYIGPCKYI APCLDSELTE FPLRMRDWLK NVLVTLYERD EGNNLLTEKQ KLRVKKIHEN EKRLEAGDHP VELLARDFEK NYNMYIFPVH WQFGQLDQHP IDGYLSHTEL APLRAPLIPM EHCTTRFFET CDLDNDKYIA LEEWAGCFGI KEQDINKDLV I
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Gene References into Functions

Data suggest that reversion-inducing-cysteine-rich protein with kazal motifs (Reck) and SPARC Glycoprotein (Sparc) seem to play a role during ovarian dynamics: Reck as a possible inhibitor and Sparc as an inducer of matrix metalloproteinases (Mmps). PMID:30424792
this study shows that SPARC paucity alleviates superoxide-mediated oxidative stress, apoptosis, and autophagy in diabetogenic hepatocytes PMID:28499910
Our in vitro and ex vivo data show osteonectin expression during the calcification process and suggest its potential role as procalcifying factor PMID:27339669
opposite expression of SPARC in the diabetic liver and pancreas may be related to inflammation and immune cell infiltration, degrees of apoptosis and fibrosis, cytoprotective machinery, and cellular insulin levels PMID:26110898
SPARC triggers a cell-autonomous program of synapse elimination in cholinergic neurons that likely occurs when protein production peaks during normal development. PMID:26420865
SPARC is involved in obesity-induced adipose insulin resistance and may serve as a potential target in the treatment of obesity and obesity-related insulin resistance. PMID:23910024
These results provide insights into age-related SPARC resistance in skeletal muscle progenitor cells, which may lead to sarcopenia. PMID:24245505
The spatio-temporal expression of Decorin, Semaphorin6D, SPARC, Spry-1 and Tsukushi in developing prostate is described and observed similar mesenchymal expression patterns in rat and human. PMID:22880013
We show that nanomolar concentrations of SPARC favour a more efficient synapse formation and increase short term depression in single cell cholinergic microcultures. PMID:22306863
These results suggest that, although SPARC plays a role in regulating skeletal muscle progenitor cells (SMPC) function, SMPCs become refractory to the action of SPARC with age. PMID:22289652
increase in SPARC may be maladaptive and lead to a progressive reduction in podocyte number, thus fueling the future development of glomerulosclerosis PMID:16093428
Osteonectin (ON) and osteocalcin (OC) was used as osteoblast markersand assessed using an indirect immunoperoxidase. technique. PMID:16443258
SPARC can change the activation state of endogenous Schwann cells, resulting in the promotion of outgrowth in vitro and in vivo. PMID:17611274
transient post-ischemic hypothermia stabilizes brain vessels and reduces blood brane barrier disruption in part by preventing proteolytic degradation of regulatory basement mambrane constituents, SPARC and agrin PMID:19285050