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Recombinant Rat SH3 and multiple ankyrin repeat domains protein 3 (Shank3), partial

Recombinant Rat SH3 and multiple ankyrin repeat domains protein 3 (Shank3), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

Shank3

Purity

>85% (SDS-PAGE)

Target Names

Shank3

Uniprot NO.

Q9JLU4

Alternative Names

Shank3; Prosap2; SH3 and multiple ankyrin repeat domains protein 3; Shank3; Proline-rich synapse-associated protein 2; ProSAP2; SPANK-2

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-1815

Target Protein Sequence

MQLNRAAVAA AAAPAEPPEP LSPALAPAPA PPGPLPRSAV GGTLAGGQGG PGRRAESPCA PLSAGNSQGP GASTGMDGPG ASAVVVRVGI PDLQQTKCLR LDPTAPVWAA KQRVLCALNH SLQDALNYGL FQPPSRGRAG KFLDEERLLQ DYPPNLDTPL PYLEFRYKRR VYAQNLIDDK QFAKLHTKAN LKKFMDYVQL HSTDKVARLL DKGLDPNFHD PDSGECPLSL AAQLDNATDL LKVLRNGGAH LDFRTRDGLT AVHCATRQRN AGALTTLLDL GASPDYKDSR GLTPLYHSAL GGGDALCCEL LLHDHAQLGT TDENGWQEIH QACRFGHVQH LEHLLFYGAN MGAQNASGNT ALHICALYNQ ESCARVLLFR GANKDVRNYN SQTAFQVAII AGNFELAEVI KTHKDSDVVP FRETPSYAKR RRLAGPSGLA SPRPLQRSAS DINLKGDQPA ASPGPTLRSL PHQLLLQRLQ EEKDRDRDGE QENDISGPSA GRGGHSKISP SGPGGSGPAP GPGPASPAPP APPPRGPKRK LYSAVPGRKF IAVKAHSPQG EGEIPLHRGE AVKVLSIGEG GFWEGTVKGR TGWFPADCVE EVQMRQYDTR HETREDRTKR LFRHYTVGSY DSLTSHSDYV IDDKVAILQK RDHEGFGFVL RGAKAETPIE EFTPTPAFPA LQYLESVDVE GVAWKAGLRT GDFLIEVNGV NVVKVGHKQV VGLIRQGGNR LVMKVVSVTR KPEEDSARRR APPPPKRAPS TTLTLRSKSM TAELEELASI RRRKGEKLDE ILAVAAEPTL RPDIADADSR AATVKQRPTS RRITPAEISS LFERQGLPGP EKLPGSLRKG IPRTKSVGED EKLASLLEGR FPRSTSMQDT VREGRGIPPP PQTAPPPPPA PYYFDSGPPP TFSPPPPPPG RAYDTVRSSF KPGLEARLGA GAAGLYDSGT PLGPLPYPER QKRARSMIIL QDSAPEVGDV PRPAPAATPP ERPKRRPRPS GPDSPYANLG AFSASLFAPS KPQRRKSPLV KQLQVEDAQE RAALAVGSPG PVGGSFAREP SPTHRGPRPG GLDYSSGEGL GLTFGGPSPG PVKERRLEER RRSTVFLSVG AIEGSPPSAD LPSLQPSRSI DERLLGTGAT TGRDLLLPSP VSALKPLVGG PSLGPSGSTF IHPLTGKPLD PSSPLALALA ARERALASQT PSRSPTPVHS PDADRPGPLF VDVQTRDSER GPLASPAFSP RSPAWIPVPA RREAEKPTRE ERKSPEDKKS MILSVLDTSL QRPAGLIVVH ATSNGQEPNR LGAEEERPGT PELAPTPMQA AAVAEPMPSP RAQPPGSIPA DPGPGQGSSE EEPELVFAVN LPPAQLSSSD EETREELARI GLVPPPEEFA NGILLATPPP GPGPLPTTVP SPASGKPSSE LPPAPESAAD SGVEEADTRS SSDPHLETTS TISTVSSMST LSSESGELTD THTSFADGHT FLLEKPPVPP KPKLKSPLGK GPVTFRDPLL KQSSDSELMA QQHHATSTGL TSAAGPARPR YLFQRRSKLW GDPVESRGLP GPEDDKPTVI SELSSRLQQL NKDTRSLGEE PVGGLGSLLD PAKKSPIAAA RCAVVPSAGW LFSSLGELST ISAQRSPGGP GGGASYSVRP SGRYPVARRA PSPVKPASLE RVEGLGAGVG GAGRPFGLTP PTILKSSSLS IPHEPKEVRF VVRSVSARSR SPSPSPLPSP SPGSGPSAGP RRPFQQKPLQ LWSKFDVGDW LESIHLGEHR DRFEDHEIEG AHLPALTKED FVELGVTRVG HRMNIERALR QLDGS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.

Gene References into Functions

Loss of scaffold proteins via knockdown of Shank1 or Shank2, but not Shank3, led to a reduction of the number but not the unitary response of AMPAR-containing synapses. Only when both Shank1 and Shank2 were knocked down, were both the number and the unitary response of active synapses reduced. This reduction was accompanied by a decrease in NMDAR-mediated synaptic response. PMID:29250591
SHANK3 expression was increased in the neocortex of temporal lobe epilepsy patients and rats. PMID:27592227
The SH3 domain from Shank3 was studied at sub-atomic resolution. The Shank family has lost the canonical peptide-binding site of the SH3 domain. The Shank SH3 domain may have lost or altered canonical SH3 domain functions. PMID:28647360
The data of this study support the concept that Shank3 is a crucial regulator of zinc-dependent signaling at excitatory synapses. PMID:27581454
NMDA-induced accumulation of Shank3 at the PSD was transient. PMID:27144302
SHANK1 and SHANK3 act as integrin activation inhibitors by sequestering active Rap1 and R-Ras via the SPN domain and thus limiting their bioavailability at the plasma membrane. PMID:28263956
In primary hippocampal cultures, Shank3 was always expressed. In stage 2 neurons, it was in soma, neuritis, and growth cones. In stage 3, it was also in the tau-positive neurites. shRNA-mediated Shank 3 knockdown upregulates GluN1 in axon terminals. PMID:26725465
Article reports a newly identified protein-protein interaction between the postsynaptic density-protein ProSAP2/Shank3 and the voltage-gated potassium channel subunit Kvbeta2 PMID:24211303
Shank3 protein has a role in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses PMID:21795692
PLCbeta1b associates with a Shank3 complex at the cardiac sarcolemma via its splice-variant-specific C-terminal tail PMID:21148417
Concerted action of ProSAP/Shank and Zn(2+) is essential for the structural integrity of the postsynaptic density and an important element in synapse formation, maturation and structural plasticity. PMID:21217644
Scaffolding proteins ProSAP1/2 were found within the postsynaptic specializations of synapses within the PNS, indicating a similar molecular assembly of central and peripheral postsynapses. PMID:20800661
results suggest that Shank recruits beta PIX and p21-associated kinase to spines for the regulation of postsynaptic structure PMID:12626503
The proline-rich region present in C termini of CaV1.3a subunit binds to Shank Src homology 3 domain. PMID:15689539
findings show that the sterile alpha motif domain of rat Shank3/ProSAP2, a master scaffolding protein located deep within the postsynaptic density, can form large sheets composed of helical fibers stacked side by side PMID:16439662
A novel binding partner for the PDZ domain of SPAR and Shank3 proteins, ProSAPiP1, is reported and characterized. PMID:16522626
Data show that Prosap2 is present in the olfactory cilia. PMID:18596612
a novel interaction partner of ProSAP2/Shank3, named ProSAP interacting protein 2 (ProSAPiP2) that does not show any close homology to other known proteins, was characterized PMID:19481056

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Subcellular Location

Cytoplasm. Cell junction, synapse, postsynaptic density. Cell projection, dendritic spine. Note=In neuronal cells, extends into the region subjacent to the PSD.

Protein Families

SHANK family

Tissue Specificity

Widely expressed in brain (at protein level).

Database Links

UNIGENE: Rn.42876

KEGG: rno:59312

STRING: 10116.ENSRNOP00000041083

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