Recombinant Rat Protein DJ-1 (Park7), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Park7

Uniprot NO.

O88767

Alternative Names

Park7; Cap1; Protein/nucleic acid deglycase DJ-1; EC 3.1.2.-; EC 3.5.1.-; EC 3.5.1.124; Contraception-associated protein 1; Protein CAP1; Fertility protein SP22; Maillard deglycase; Parkinson disease protein 7 homolog; Parkinsonism-associated deglycase; Protein DJ-1; DJ-1

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage. Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Also displays an apparent glyoxalase activity that in fact reflects its deglycase activity. Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function. It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells. In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity. In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis.

Gene References into Functions

DJ-1 is necessary for Resveratrol-mediated cardioprotective effects. PMID:29287203
DJ1 -/- rats cross a tapered balance beam with significantly decreased speed of traversal PMID:29109055
The work revealed that preserving mitochondrial complex I activity and subsequently inhibiting mitochondrial ROS generation could be a novel mechanism by which DJ-1 mediates the cardioprotection of DHP against H/R-induced oxidative stress damage. PMID:29431188
chaperone-mediated autophagy protects mitochondrial function by degrading nonfunctional PARK7 and maintaining its homeostasis, and dysregulation of this pathway may contribute to the neuronal stress and death in Parkinson disease pathogenesis PMID:27171370
Low DJ-1 expression is associated with pulmonary arterial hypertension. PMID:29069575
Results suggest that overexpression of DJ-1 improves mitochondrial function, at least in part, through a mechanism involving Akt phosphorylation on threonine 308. PMID:26913805
DJ-1 activates autophagy to alleviate cardiac hypertrophy. PMID:28941803
The present study identified DJ-1 as an essential mediator responsible for the beneficial effects of the late phase of ischemic preconditioning in vivo. In addition ischemic preconditioning exerts delayed cardioprotective effects by a hitherto unrecognized underlying mechanism; the attenuation of ischemia/reperfusion -induced oxidative stress via the upregulation of DJ-1. PMID:28035392
Data show that high glucose (HG) for 48 h and then exposed to hypoxia for 4 h and reoxygenation for 2 h significantly decreased cell viability in NRK-52E proximal tubular epithelial cells accompanied by a decrease in DJ-1-PARK7 Protein (DJ1) expression. PMID:27430285
Cardioprotection in a cellular model of hypoxic preconditioning is abolished with knockdown of DJ-1. PMID:25915512
Data indicate that females showed greater susceptibility towards developing streptozotocin-induced diabetes because of lower insulin secretion and higher blood glucose levels. PMID:25726699
DJ-1 plays an important role in caffeic acid-mediated protection against ketoprofen-induced oxidative damage in the GI mucosa. PMID:25037998
phenotypic characterization of Pink1, DJ-1 and Parkin recessive gene knockout Parkinson's disease model; results show inactivation of Pink1 or DJ-1 genes produces progressive neurodegeneration and behavioral deficits, suggesting these recessive genes may be essential for the survival of substantia nigra dopaminergic neurons PMID:24969022
distinctive changes in the expression of dopamine presynaptic markers and receptors in the striatum may reflect different compensatory regulation of dopamine system in DJ-1 versus Pink1 knockout rat models of familial parkinson's disease PMID:24157858
Downregulation of cardiac DJ-1 may be responsible for the compromised diabetic heart responsiveness to Ischemia postconditioning cardioprotection. PMID:24303086
Results show DJ-1 protein associated with lipid rafts. This novel function of DJ-1 in lipid rafts, may contribute the pathogenesis of Parkinson's Disease. PMID:23847046
The results of this study suggested that DJ-1 is closely associated with the differentiation of neural stem cells. PMID:22613231
Electrophilic p-quinone formed from 6-hydroxydopamine induces DJ-1 oxidation by decreasing intracellular glutathione. PMID:22132160
DJ-1 induces Akt/PRAS40/TORC1-mediated hypertrophy in response to high glucose. PMID:21426932
The DJ-1 acts with PINK1 in a common pathway to regulate NMDAr-mediated neuronal death. PMID:20698836
DJ-1 protein is predominantly expressed by neurons but can also be detected in astrocytes. PMID:16047164
SP22 was localized to specific cell types within the anterior pituitary gland. PMID:16246899
DJ-1 mRNA expression was restricted mainly to neurons in all brain regions analyzed. PMID:17599367
expression of SP22 in the rat ovary is most prevalent in the corpora lutea and increased during pregnancy PMID:17609507
DJ-1 protects through alleviation of oxidative stress PMID:18003894

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Subcellular Location

Cell membrane; Lipid-anchor. Cytoplasm. Membrane raft. Nucleus. Mitochondrion. Endoplasmic reticulum.

Protein Families

Peptidase C56 family

Tissue Specificity

Ubiquitous. Detected on epididymal sperm. Highly expressed in testis and prostate. Detected at lower levels in heart, lung, brain, liver, kidney, seminal vesicle, caput and corpus epididymis.

Database Links

UNIGENE: Rn.30105

KEGG: rno:117287

STRING: 10116.ENSRNOP00000024711

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