Recombinant Rat PRKCA-binding protein (Pick1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

Pick1

Purity

>85% (SDS-PAGE)

Target Names

Pick1

Uniprot NO.

Q9EP80

Alternative Names

Pick1; Prkcabp; PRKCA-binding protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-416

Target Protein Sequence

MFADLDYDIE EDKLGIPTVP GKVTLQKDAQ NLIGISIGGG AQYCPCLYIV QVFDNTPAAL DGTVAAGDEI TGVNGRSIKG KTKVEVAKMI QEVKGEVTIH YNKLQADPKQ GMSLDIVLKK VKHRLVENMS SGTADALGLS RAILCNDGLV KRLEELERTA ELYKGMTEHT KNLLRAFYEL SQTHRAFGDV FSVIGVREPQ PAASEAFVKF ADAHRSIEKF GIRLLKTIKP MLTDLNTYLN KAIPDTRLTI KKYLDVKFEY LSYCLKVKEM DDEEYSCIAL GEPLYRVSTG NYEYRLILRC RQEARARFSQ MRKDVLEKME LLDQKHVQDI VFQLQRFVST MSKYYNDCYA VLRDADVFPI EVDLAHTTLA YGPNQGGFTD GEDEEEEEED GAAREVSKDA RGATGPTDKG GSWCDS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.

Gene References into Functions

Results suggest that the interacting proteins PICK1 and N-ethylmaleimide sensitive fusion protein act as regulators of presynaptic GluA2-containing AMPAR trafficking between the active zone and a vesicle pool that may provide the basis of presynaptic components of synaptic plasticity. PMID:28057533
PICK1 is a cargo-specific endocytic accessory protein required for efficient, activity-dependent AMPAR endocytosis. PMID:28855251
This study defines a novel mechanism whereby elevated [Ca(2+)] induced by NMDA receptor activation modulates Ago2 and miRNA activity via PICK1. Our work suggests a Ca(2+)-dependent process to regulate miRNA activity in neurons in response to the induction of long-term depression. PMID:28404816
syntabulin forms a complex with PICK1 and ASICs, regulates ASIC protein expression in neurons, and participates in ASIC-induced acidotoxicity. PMID:26868290
ICA1L forms BAR-domain complexes with PICK1 and is crucial for acrosome formation in spermiogenesis. PMID:26306493
PICK1 binds Rac1 and Cdc42, via distinct but overlapping binding sites PMID:25475687
PICK1 interacts with parkin and our data showed that PICK1 reduces PAELR expression levels in transiently transfected heterologous cells PMID:24749734
The Arp2/3 regulators PICK1, and N-WASP and WAVE2 function antagonistically to control the complexity of astrocyte branched morphology, and this mechanism underlies the morphological changes seen in astrocytes during their response to pathological insult. PMID:23843614
It is a binding partner of GHRHR and controls cell surface expression and signal activity of the receptor. PMID:23823934
Modulation of PICK1 regulates actin dynamics and synaptic function. PMID:23889934
a shift of PICK1 expression from motor neurones in healthy rats to activated astrocytes in animals with end-stage amyotrophic lateral sclerosis point to a potential implication of PICK1 in the progression of this disease PMID:22624977
The data of this study demonstrated that a PICK1-GRIP1-GluA2 complex is involved in the regulation of GluA2 trafficking after glycine stimulation and therefore suggest that this complex is involved in these processes during LTP expression. PMID:22915106
PICK1 is phosphorylated at Ser77 by PKCalpha preferentially when bound to membrane vesicles PMID:22129425
Via Arp2/3 inhibition, PICK1 has complementary yet distinct roles during LTD to regulate AMPA receptor trafficking and spine size, and therefore functions as a crucial factor in both structural and functional plasticity. PMID:21252856
Results indicate that PICK1 regulates trafficking and function of ASIC1a in a lipid binding-dependent manner. PMID:21176140
PICK1 may function in the regulation of synaptic transmission and/or plasticity. PMID:12209850
phosphorylation of p46 (PICK-1) by GTP and Ca(2+)/CaM may be important for the regulation of transporters and neurosecretion PMID:12526085
Surface expression of the netrin receptor UNC5H1 is regulated by Protein interacting with C kinase-1 in neurons. PMID:14672991
PICK1 has a role in ASIC2b regulation of ASIC3 PMID:14976185
These findings suggest that PICK1 acts to downregulate the GluR2 content of AMPARs at hippocampal CA1 synapses, thereby increasing synaptic strength at resting membrane potentials PMID:15190111
results define the structural basis for the unusual binding pattern of the PICK1 (protein interacting with C kinase 1) PDZ domain by substantiating the critical role of the alphaB1 position (Lysine(83)) PMID:15774468
Repetitive synaptic activity triggers loss of synaptic GluR2-lacking AMPARs by selectively disrupting their interaction with GRIP and that PICK drives activity-dependent delivery of GluR2-containing receptors PMID:15895086
Our results show that PICK1 localization in dendrites is subject to multiple forms of regulation that contribute to surface AMPAR expression, likely by modulating the numbers of AMPARs maintained in intracellular compartments. PMID:16406232
PICK1 synaptic targeting requires direct interaction between the PDZ domain and lipid membranes PMID:17914463
The switch from islet cell autoantigen 1 (ICA69)-PICK1 heteromeric complexes to PICK1-PICK1 homomeric complexes could be an important mechanism regulating synaptic targeting and surface expression of AMPA receptors. PMID:18032668
The trafficking of PICK1 from the cytoplasm to cell membrane is regulated by a single zinc2+-binding site. PMID:18429931
in the absence of PDZ ligand, the PICK1 BAR domain is inhibited through a PDZ domain-dependent and linker-dependent mechanism PMID:18466293
can suppress both the basal activity of the Arp2/3 complex as well as its activation by nucleation-promoting factors PMID:18492472
PICK1 is involved in the regulation of presynaptic AMPA receptor trafficking and in determining the size of the AMPA receptor pool that modulates presynaptic glutamate release. PMID:19071197

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Subcellular Location

Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Membrane; Lipid-anchor. Cell junction, synapse, postsynaptic density. Cell junction, synapse, synaptosome. Cytoplasm, cytoskeleton. Note=Also membrane-associated, present at excitatory synapses.

Tissue Specificity

Ubiquitous.

Database Links

UNIGENE: Rn.24750

KEGG: rno:84591

STRING: 10116.ENSRNOP00000016077

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