Recombinant Rat Otoferlin (Otof), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Target Background

Product Details

Abbreviation

Otof

Purity

>85% (SDS-PAGE)

Target Names

Otof

Uniprot NO.

Q9ERC5

Species

Rattus norvegicus (Rat)

Source

Yeast

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes.

Gene References into Functions

otoferlin, through GAD65, modulates GABAergic activity. PMID:25701657
a direct role for otoferlin in exocytosis and modulation of calcium-dependent membrane fusion PMID:24478316
otoferlin-AP-2 interaction drives Ca(2+)- and stimulus-dependent compensating clathrin-mediated endocytosis in mature inner hair PMID:23719817
Otof CA domain differs structurally and functionally from other C(2) domains PMID:21216247
Otoferlin mRNA and protein was detected in mature outer hair cells of low-frequency processing cochlear turns and in auditory nerve fibres. In outer, inner and vestibular hair cells PMID:17229086
Using a rodent cochlear cDNA library Rab8b is identified as an interacting partner of otoferlin. PMID:18772196

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Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Cell junction, synapse, presynaptic cell membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein.

Protein Families

Ferlin family

Tissue Specificity

Isoform 1 is expressed in the cochlea and brain. Expressed in cerebellum (Purkinje cells), hippocampus (granule cells of the dentate gyrus and in pyramidal cells of the CA1-CA3 region) and cortex (stellate and pyramidal cells). Expressed in hair cells of

Database Links

UNIGENE: Rn.148993

STRING: 10116.ENSRNOP00000046997

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