Recombinant Rat Non-muscle caldesmon (Cald1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

Cald1

Purity

>85% (SDS-PAGE)

Target Names

Cald1

Uniprot NO.

Q62736

Alternative Names

Cald1Non-muscle caldesmon; CDM; L-caldesmon

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-531

Target Protein Sequence

MLSRSGSQGR RCLATLSQIA YQRNDDDEEE AARERRRRAR QERLRQKQEE ESLGQVTDQV EAHVQNSAPD EESKPATANA QVEGDEEAAL LERLARREER RQKRLQEALE RQKEFDPTIT DGSLSVPSRR MQNNSAENET AEGEEKGESR SGRYEMEETE VVITSYQKNS YQDAEDKKKE EKEEEEEEEK LKGGNLGENQ IKDEKIKKDK EPKEEVKNFL DRKKGFTEVK AQNGEFMTHK LKQTENAFSP SRSGGRASGD KEAEGAPQVE AGKRLEELRR RRGETESEEF EKLKQKQQEA ALELEELKKK REERRKVLEE EEQRRKQEEA DRKAREEEEK RRLKEEIERR RAEAAEKRQK MPEDGLSEDK KPFKCFTPKG SSLKIEERAE FLNKSVQKSG VKSTHQAAVV SKIDSRLEQY TNAIEGTKAS KPMKPAASDL PVPAEGVRNI KSMWEKGSVF SSPSASGTPN KETAGLKVGV SSRINEWLTK SPDGNKSPAP KPSDLRPGDV SGKRNLWEKQ SVDKVTSPTK V
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration.

Gene References into Functions

distribution of the actin-binding protein caldesmon in spleen and lymph nodes of mice and rats PMID:19875849
CaD stabilizes the F-actin dynamics in spines, thereby enlarging the spine heads, whereas CaD knockdown decreases the spine-head size via destabilization of the F-actin dynamics. PMID:23077044
caldesmon is an important regulator of axon development. PMID:22158623
Overexpression of l-CaD suppressed cell growth and survival in VSMCs and inhibited neointimal formation after angioplasty, partly by regulating cytoskeletal tension-FAK-ERK axis. ) PMID:16888241
Caldesmon binds and stabilizes actin filaments, as well as regulates actin-myosin interaction in a calcium (Ca2+)/calmodulin (CaM)- and/or phosphorylation-dependent manner. (Review) PMID:21350330
Stretch activates myometrium via ERK, caldesmon and focal adhesion signaling PMID:19834610
ERK1/2-mediated phosphorylation of myometrial caldesmon during pregnancy and labor PMID:12388473
caldesmon was most markedly expressed in bladder smooth muscle after partial outlet obstruction PMID:12757606
The smaller C-terminal region contains the minimum regulatory domain responsible for the inhibition of actomyosin ATPase, and for the binding to actin, calmodulin and TM. PMID:16428310
Data suggest that caldesmon may play a role in the regulation of the dynamics of podosome assembly and that Ca2+/calmodulin may be part of a regulatory mechanism in podosome formation. PMID:16595550
in Rous sarcoma virus-transformed cells, changes in the balance between PAK1/2-regulated caldesmon and the Arp2/3 complex govern the formation of podosomes PMID:17224451
Caldesmon suppresses cancer cell invasion by regulating podosome/invadopodium formation. PMID:17631293
This study reports a novel molecular mechanism (caldesmon), as well as a structural basis (podosomes/rosettes), to show how p53 can act as an anti-motility/invasion/metastasis agent. PMID:19349302

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Subcellular Location

Cytoplasm, cytoskeleton. Cytoplasm, myofibril. Cytoplasm, cytoskeleton, stress fiber.

Protein Families

Caldesmon family

Tissue Specificity

High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Database Links

UNIGENE: Rn.204926

KEGG: rno:25687

STRING: 10116.ENSRNOP00000043767

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