Recombinant Rat Myelin basic protein (Mbp)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Mbp

Uniprot NO.

P02688

Alternative Names

MbpMyelin basic protein; MBP

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

2-195

Target Protein Sequence

ASQKRPSQR HGSKYLATAS TMDHARHGFL PRHRDTGILD SIGRFFSGDR GAPKRGSGKV PWLKQSRSPL PSHARSRPGL CHMYKDSHTR TTHYGSLPQK SQRTQDENPV VHFFKNIVTP RTPPPSQGKG RGLSLSRFSW GAEGQKPGFG YGGRASDYKS AHKGFKGAYD AQGTLSKIFK LGGRDSRSGS PMARR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Buffer

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I wanted to know what cell line was used to produce the MPB (ref number: CSB-MP365771RA).

Thanks for your inquiry,The cell line we usually use is HEK293T cell.Except that, we could express the protein in CHO cell. If you need specific cell line, please let me know, and mark it on the P.O when place the order.Thanks.

I entend to use CSB-EP365771RA and CSB-EP015513RA as antigens, to develop targetting molecules. Do you know if by beeing recombinant they would differ in conformation from the native proteins? And would there be any advantage in buying them from a mamalian cell (CSB-MP365771RA and CSB-MP015513RA)?

Recombinant proteins differ from native proteins. The target proteins prepared by various expression systems also exhibit certain differences.

Proteins expressed in E. coli expression systems undergo minimal post-translational modifications.
Proteins expressed in yeast systems can fold effectively, undergo post-translational modifications, and may exhibit glycosylation.
Proteins expressed in insect systems fold effectively and undergo extensive post-translational modifications.
Proteins expressed in mammalian systems are suitable for secretion, ensuring correct modifications and proper folding.

Target Background

Function(From Uniprot)

Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane.

Gene References into Functions

Angiostrongylus cantonensis-conditioned culture medium induced suppression of the Erk1/2-NF-kappaB signaling pathway leading to reduced MBP production in RSC96 Schwann cells. PMID:29742816
A role for the retrograde dynein/dynactin motor complex in anterograde mbp mRNA transport. PMID:29073112
The aberrant phase transition of MBP molecules from their cohesive to soluble and non-adhesive state is a mechanism triggering myelin breakdown in neuromyelitis optica. PMID:27346352
hindlimb unweighting treatment induced damage of L5 DRG neurons, which was correlated with decreased total MBP protein expression, increased dgen-MBP expression, and reduced GDNF protein and mRNA expression PMID:27230884
Bilateral common carotid artery occlusion led to loss of myelin basic protein expression. PMID:26670084
Study demonstrated that downregulation of tau severely impairs process outgrowth, oligodendrocyte differentiation, and sheet formation, and that MBP mRNA transport to the distal cellular extensions is disturbed PMID:25847153
Protein levels of MBP were significantly greater in orbital frontal cortex (OFC) but not dorsal striatum (dSTR) of females as compared to males, suggesting that myelination is increased in OFC but similar in dSTR of females as compared to males PMID:26002313
MBP cell membrane localization is altered in the presence of galactolipids. PMID:25003183
Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4. PMID:25512606
The expression of myelin basic protein was detected in accordance with the radially extending myelinated processes of the neurons in the cortex. PMID:25282817
Given the principle of the longest time window and the lowest therapeutic dose, the optimized therapeutic dose and time window should be injecting picroside II intraperitoneally with 10-20 mg/kg body weight at ischemia in cerebral ischemic injury. PMID:24524292
The e-MBP isoform is exclusively present in OLN-93 cells. PMID:24321769
MBP epitopes normally sheltered from immunosurveillance may induce MBP-specific T cell clones and a self-sustaining immune reaction, which may together contribute to transition of acute pain into chronic neuropathic pain state. PMID:22676642
Methylmercury-induced perturbation in Mbp gene expression occurred as a consequence of decreased oligodendrocyte cell population in absence of a significant impact on its post-transcriptional regulation. PMID:22835759
Morphological basis of spinal cord recovery from debilitating experimental neurodenerative disease is evidenced by robust myelin basic protein (MBP) expression and extensive remyelination. PMID:21996274
The impact of Hoxa2 over-expression on the proliferation and differentiation of CG4-SHoxa2 cells was investigated. PMID:21479584
MBP synthesis is regulated by hnRNP-K and integrin activation. PMID:21357748
Myelin basic protein expression decreases following demyelination of optic nerve and chiasm and then increases to control levels 14 days post-lesion. PMID:20637745
Sp1 phosphorylation can be regulated by PKC-ERK pathways. This phosphorylation is important for MBP transcription and oligodendrocyte differentiation PMID:20882567
Increasing the negative surface charge of the cell membrane reduces the amount of Fyn-SH3 domain binding to membrane-associated MBP. PMID:19178193
Phosphatidylinositol-3 kinase p85 enhances expression from the myelin basic protein promoter in oligodendrocytes independent of PI3K activity and dependent on the adapter functions of the p85 subunit's SH2 domains PMID:12390518
isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform. The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. PMID:14580679
Two regulatory elements in the promoter interact with Nkx2.2 in vitro PMID:15695521
Susceptibility or resistance to EAE is not regulated by the amount of the neuroantigens expressed in the thymus. Furthermore, the lesion distribution, but not the clinical course, of EAE is related to the neuroantigen expression in the CNS. PMID:16773652
Mbp is a binding partner and calmodulin adaptor for the BKCa channel. PMID:17610306
21.5 kDa molecule expressed during [dental] hard tissue formation PMID:18821983
study demonstrates co-localisation of MBP & myristoylated alanine-rich C kinase substrate (MARKS) in oligodendrocytes, & co-distribution of MBP, MARCKS & GAP-43 in lipid raft fractions recovered from myelin & brain homogenates PMID:19026719
Myelin basic protein-reactive T cells persist in an inactive state in the bone marrow of Lewis rats that have recovered from autoimmune encephalomyelitis. PMID:19211156