Abbreviation
Recombinant Rat Il1rap protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat Il1rap protein at 2 μg/mL can bind Anti-Il1RAP recombinant antibody (CSB-RA878844MA2HU). The EC50 is 3.180-4.082 ng/mL.
Alternative Names
Interleukin-1 receptor accessory protein; EC:3.2.2.6; IL-1 receptor accessory protein; IL-1RAcP; Il1rap
Species
Rattus norvegicus (Rat)
Expression Region
21-367aa
Target Protein Sequence
SERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKYNYSTAHSSGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMRLPVHRLYIEQGIHNITCPNVDGYFPSSVKPSVTWYKGCTEIVNFHNVQPKGMNLSFFIPLVSNNGNYTCVVTYLENGRLFHLTRTMTVKVVGSPKDAVPPHIYSPNDRVVYEKEPGEELVIPCKVYFSFIMDSHNEIWWTIDGKKPDDVPVDITIIESVSYSSTEDETRTQILSIKKVTPEDLKRNYVCHARNAEGEAEQAAKVKQKVIPPRYTVELACGFGAT
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat Il1rap protein at 2 μg/ml can bind Anti-Il1RAP recombinant antibody (CSB-RA878844MA2HU). The EC50 is 3.180-4.082 ng/mL.
Description
IL-1 receptor accessory protein forms an essential co-receptor complex with IL-1R1 to transduce pro-inflammatory signals, making it a critical target for dissecting cytokine signaling mechanisms and screening anti-inflammatory therapeutics. This mammalian-expressed construct spanning residues 21–367 captures the extracellular domain responsible for receptor assembly and ligand recognition, with native post-translational modifications that preserve conformational epitopes required for antibody interaction studies. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 3.180–4.082 ng/mL, confirming that the protein maintains its native structure and supports applications including therapeutic antibody epitope mapping, competitive inhibition screening of IL-1 pathway antagonists, and affinity characterization by surface plasmon resonance or biolayer interferometry. The combination of >95% purity and <1.0 EU/μg endotoxin satisfies the quality criteria typical for quantitative binding assays and cell-based functional studies where low endotoxin interference is essential.
