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Recombinant Rat Hormone-sensitive lipase (Lipe), partial

Recombinant Rat Hormone-sensitive lipase (Lipe), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Lipe

Uniprot NO.

P15304

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

1-1068

Target Protein Sequence

MKPRRPISFT REITAMEPSS TSVSRPEWRP EAQQTLTDYP GSRELQEFGI PQKQSLPNEA TAQQGAEFQQ EQGVQQSTLL QKLLTPLAFP VPQQSFPSHK VHSDQQEATS QNGPGAGKVH TTQKELEHRD EHVGTAESGP AEPPPATEVE ATSIAQAVSG PDKKLPTQTD LVSQERAEQS DPTAQQTPLV QGVKSDQGSL IESGILARLQ KLAIQQPSQE WKTFLDCVTE SDMEKYLNSS SKSNPPEPSG GTVIPGTLPS KQKPDCGKMS GYGGKLPHGK KGILQKHKHY WDTASAFSHS MDLRTMTQSL VALAEDNMAF FSSQGPGETA RRLSNVFAGV REQALGLEPT LGQLLGVAHH FDLDTETPAN GYRSLVHTAR CCLAHLLHKS RYVASNRRSI FFRASHNLAE LEAYLAALTQ LRALAYYAQR LLTINRPGVL FFEGDEGLSA DFLQDYVTLH KGCFYGRCLG FQFTPAIRPF LQTLSIGLVS FGEHYKRNET GLSVTASSLF TGGRFAIDPE LRGAEFERII QNLDVHFWKA FWNITEIEVL SSLANMASTT VRVSRLLSLP PEAFEMPLTS DPKLTVTISP PLAHTGPGPV LARLISYDLR EGQDSKMLNS LAKSEGPRLE LRPRPQQAPR SRALVVHIHG GGFVAQTSKS HEPYLKNWAQ ELGVPIISID YSLAPEAPFP RALEECFFAY CWAVKHCELL GSTGERICLA GDSAGGNLCI TVSLRAAAYG VRVPDGIMAA YPVTTLQSSA SPSRLLSLMD PLLPLSVLSK CVSAYSGTET EDHFDSDQKA LGVMGLVQRD TSLFLRDLRL GASSWLNSFL ELSGRKPHKT PLPATETLRP TDSGRLTESM RRSVSEAALA QPEGLLGTDS LKKLTIKDLS FKGNSEPSDS PEMSQSMETL GPSTPSDVNF FLRSGNSQEE AETRDDISPM DGIPRVRAAF PDGFHPRRSS QGVLHMPLYS SPIVKNPFMS PLLAPDVMLK TLPPVHLVAC ALDPMLDDSV MFARRLKDLG QPVTLKVVED LPHGFLSLAA LCRETRQAAE LCVQRIRLIL TPPAAPLT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of DAGs. Preferentially hydrolyzes fatty acids at the sn-1 and sn-2 positions of TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

Gene References into Functions

Habitual exercise training acts as a physiological stimulator for constant activation of lipolytic enzymes, HSL and ATGL, in rat primary white adipocytes. PMID:26141235
The findings suggest that progesterone, acting in a progesterone receptor-dependent manner, down-regulates Lipe gene expression but exerts no effect on Pnpla2 gene expression in the inguinal white adipose tissue of females. PMID:25448749
These findings suggest that acute exercise enhances lipolysis up to at least 3h after exercise in a manner dependent on modification of HSL and its association with and alteration in scaffold protein. PMID:20708600
When compared with obesity-resistant rats, the gene expression of HSL in white adipose tissue was lower in obesity-prone rats. PMID:17712951
hormone-sensitive lipase is involved in mediating beta-cell lipotoxicity by providing ligands for peroxisome proliferator-activated receptors and other lipid-activated transcription factors, which in turn alter the expression of critical genes. PMID:12882923
interaction of HSL with StAR in cytosol increases the hydrolytic activity of HSL and that together HSL and steroidogeneic acute regulatory protein facilitate cholesterol movement from lipid droplets to mitochondria for steroidogenesis PMID:12925534
an 89-kDa isoform of hormone-sensitive lipase (HSL) and its molecular structure is described; the novel HSL isoform is expressed in the pancreatic beta-cell, adipose tissue, adrenal gland, and ovary PMID:14576146
hormone sensitive lipase is phosphorylated by 5' AMP-activated protein kinase at Ser(565) during muscle contractions, but this phosphorylation is not directly responsible for the contraction-induced activation of HSL PMID:15033481
Immunoreactive hormone-sensitive lipase (HSL) and HSL activity were found in lactating rats' milk PMID:15654127
The length of the regulatory module and specific sequences within the C-terminal portion of the regulatory module of hormone sensitive lipase (amino acids 644-683) are crucial for activity and appear to be responsible for determining lipase activity. PMID:15697220
Upregulated by rosiglitazone. PMID:15887043
HSL labeling in the nucleus of epithelial and germ cells suggests an as yet unknown function for this protein, probably related to oogenesis and cell proliferation. PMID:18824635
The retinyl ester hydrolase activity of rat HSL was found to be higher than that against the hitherto best known HSL substrate, i.e., diacylglycerols. PMID:19246492
PKA phosphorylation induces a conformational change that increases the exposed hydrophobic surface and thereby facilitates binding of HSL to the lipid substrate. PMID:19664063